ISfinder Mirror Site

ISPa38

Family Tn3
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) TnPa38

Accession number	Transposition	Origin	Host
	ND	Pseudomonas aeruginosa	Pseudomonas aeruginosa DK2

DNA section

IS Length : 6455 bp

Ends

IR Length : 39

IRL : GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAGCGTGCGTGGAGG
IRR : GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAGCCGGTTGCAGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGTGTTGGTA		TACCTGAAAG	0

DNA sequence

GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAGCGTGCGTGGAGGCTGGCACCCAGCGGTACAGCGTCGGAATGGACACGCCGAGGTTCTTGGCC
ACGTCCTTGGGCGGCACCCCGCTGGCCAGCAGCTTCTTGGCCGACTCGATCTTGCTGTCGGTCATCTTCGGCTTGCGGCCGCCTTTGCGGCCGAGCTGCT
TGGCGACTTCCAGCCCGGCGCGGGTGCGCTCGACGGTCAGCTCGCGCTCCATTTCGGCAAGGCTCGCCATGACGTGGAAGAAGAACCGCCCGGATGGTGT
GCCGGTGTCGATGGAGTCGGTGAGGCTCCTGAACTGGACACCGTGCTTGTGCAGATCGCCGACCAGATCGACCAGTTGCTTGACCGACCGGCCCAGCCGG
TCGAGCTTCCAGACGACCAAAGTATCGCCTTCGCGCAGCATTTCGAGCGTCTTGGCCAAGCCAGGCCGGTCTGCCCGCGTGCCACTCACCTTGTCCTCGA
AGACCTTTTTACATCCGGCCTTGCTCAAGGCTTCGCGTTGCAGCTCCAGGTTCTGATCCTGCGTCGAGACGCGCGCATAGCCAATCAACATGGCTTGTCT
CGCGCCCGGTCGATGCGTTCCTGCATCGCCTGCATCACTTCTTCGTGGGTGTACGATCTGGCGTTGGCGTCGGTGGCTTGCCGCAGGGCTTCCTCAACCT
CGCGCGTCATCCGCTCGTAATCCTCCGGCCACAGCGCTTGCTCCATGCGCAGCGACGCCTGACCCAGCAGGTGCAGCAGGCTGAACAGCCGCATGTCGTT
GGTGGCGACGATGCGCTCCCGAATCTGCTCCTGAATAGCTTCGCTAGCCCGATGCGCTTGTGGTGTGGCAGTCCCCTCGTAGTCAGCGGGGAATTCCGCT
TCCTCGGCAATCCTTGCCCAGGCGCTGGCCAGCGCCTCGATGGTTGACGTGCTCATTTCCGCCGCTCCTGTGCTCTTTGGCGAATCAACCGGCCAAGGGG
CCTCGACGCGAAAACCAGCAGCATCACGCCTATCGGATACCAGGTCGAGAAGACCACCAAGGCATCGAAGGCTGGCCGTCCGGTGTTGGTAGGCAGTACG
GCGGTCACAGCCATCAACCCGCCGACCGTCCAGATGATGCGCCACACGTAGGGCATCACGCGGGGCACGTAGCCGTCATCGAAAGCGGTCTGGTAGTAGC
GGCGCAGGCCGCGCTCGGCAAGCGCCTGGCGCTGCCGCTCCGACAGCGCATCCGTCCGGCCATACCAGCGCCGGAATGGTGGACAGCGCAGCAGCAAAGG
GGTGAAGAGGATCATCACCGCCACGATCGCGACACCCCACGCCATGACGGCGCCGGCATCGGGCCGCTTGGCGTTCTCGATCACGGGCGCGAAGACGCCC
GGAGCACCGATCATCCAGAACAGCGCAATGTGCTGATGGAAGGAGAGCTTCATTTGCTCATCCACTTGCGCAGCAGGCGCTTTTTCAGGGAGGCGCGTTC
TTGCGGGTTGCGTCCCTTGTGATTGGCGATGCGATCCGCCGTGGCGGCCTGGCGCTTGACGGGCCAGTAGGAGCGGCCATCCTTGCCCATCGACCAGACG
CTGCTGGCCTGGTTTTCCAGCAGGGGCAGATGGGCGCTCAGGGCTTCGGGCGACGCGCTGGTCAGCGCCGTGCGCTCACGGGTGCGCCAGCGCTGATGCC
AGAGCTTCTTGTCCTCGCGCTCGCTGCCGCAGGTCGTGTGCCCGACGATGGGTGTTTTGCGGCGGCTGCGGCTCATAACGTAGTGGTCTCCAAGAACATT
CAGGACTGATCCCAGGCGTCGATGGTCAAGACCTGATCGGCAGGACAGGCGGCTACGCGGTCGGGAACTGGATGGTGTTCAGTCTCATGCCGACCCCATT
CGATTGATCGCGTCGCTTGCGGCACGCTGCGACGCAAGGAGGTTTGCGACCTGGTTTAGCAGCCGTGTCCGCTGCATGTCTGTTACCTATCTCCCCGACC
GCTCATTGGACCAACTCCAGAGATTGGGCTCTATCGCTCAGCCAATACGAAACCGGCATTGGCTGATGCCACAACCGAGACGAACACAAATGGCTCGGTA
CCTGTATTTCGCGCCCCGTGCACTTGGCCCGGTCTTGCCACGGCTATCTCACCTTCCCTGAGGGCACGAACAATCCCATTGCCCTGAAAGTAATCAGCCA
TTCCCGACAAAACAGTCCACGTGTCTTGGCCGTGAGGATGAATGTGAGCCGCAATTTCCTGCCCGGGATGGACATGCCAAACCACGATAATTGAGTCTCG
GGTTTCAAGCACAACGGAACGAATAGGCTCGCCTTCGGACGGCTGAACATACTCGGCTACAGAAAATATTCTCGATTCAACAGTCATCGGAGATCCCTCT
TTCACAGTGCCCCCAGACAGGCTGGATATGAGTTCTAACTCGAATTTGAACGGGAGGCTGGTCGTGCGGTCGTGCAGTTGCCGATGAGCGTGTCGGCTGC
TGCCTCCTTGCCCACCAGCGAACTCACGTCCGTTGCGGCCATCCAGAAGGTCTTGCCCGAGCGCGGCTCATAGGTCGCGGGATCGAACACGCCAGAGGGG
CCGAACATCGGCGGCTTGATTGGTCATGGCTCCATGCCTTTGTCGTTACGGTCTTCATCGTCGGCATCCTGACGCACGGCGGGCAATTGCTGGAGCAACG
CCGGCAGCCATTCCTGTACCGTCTCCCACACCACATCGAGGTTGATGTCGAAATAGCCGTGAGCCATGCGATTACGCATATTGCGCATGCTGCGCCACGG
CACGTCGGCATGCGCCTGGGTGAACTCGACGTAGCCATCCATCACCTTTGTGGCCGCCTCGCCGATGACGATCAGGCTCATGATGACGGCCTGCTGGGTG
CGCTTGTCGGCCAAGAAGTCGTCCTTGGCCATCCCTTCCACGAAGCTGCGCGCATCGGTTGCGGCCTGCTGAATGTGGTCGAGGTAATCGGGCAGGCGGT
TCTCGCTCATATCGGTTGCGCCTCCGCGAGCACCTTGGCCCGGAACTTCGGCGGCAGGTCGCCGGGAGTCAGCAGATCGACGTCAACGCCGAGCAGCGAT
TTCAGTTCTTCTTCCAAATCGCCCAAGTCCAACAACGTGGCACCGGGCAGCGCATCGACCAACAGGTCGAGGTCGCTGCCATCCCGGTCGGTGCCATGCA
GCACCGAGCCGAAGACGCGCGGGTTCGCGGCGCGAAAGCGGCCTACCGCTTCACGCACTGCGCTTCGCTTCATGTCAAGCACAACAGACGGTCGCATGGG
CATCCTTTCTTATCGAAACTCGTTGAGATGATATGCAATCAAGAATAGAATTTCAAGAACTATTTTCGAGAATCGCAATGCCTTGATTCCCGTGCCGCGC
CGGTTGCCTTGGCGGGCTTGTCACAAACCTACGTTTTCAAGAAGAAGGAAACCGCATGCCCCGCCGTTCGATCCTCTCCGCCGCCGAGCGCGAAAGCCTG
CTGGCGTTGCCGGACACCAAGGATGAGTTGATCCGTCACTACACGTTCAGCGAAAGCGACCTCTCCATCATCCGGCAGCGGCGCGGCCCGGCCAATCGGC
TGGGCTTCGCGGTGCAGCTCTGCTACCTGCGCTTTCCCGGCGTCATCCTTGGCGCTGATGAGCCACCGTTCCCGCCATTGCTGAGACTGGTCGCCAACCA
GCTCAAGGTCGGCATCGAAAGCTGGGACGAGTACGGGCAGCGTGAGCAGACCCGACGCGAGCACCTGGTCGAGCTGCAAACGGTGTTCGGCTTCCAGCCG
TTCACGATTGGCCACTACCGGCAGGCTGTCCAGTTGCTGACCGAGCTGGCCATGCAAACCGACAAGGGCATCGTGCTGGCCAGAGCCTTGATCGAGCACC
TGCGGCGGCAGTCGGTCATTGTGCCCGCCCTCAACGCCGTCGAGCGGGCGAGCGCCGAAGCGATTACCCGCGCCAACCGGCGTCTCTACGACGCCTTGGC
TGAGCCGCTGACGGACGTGCATCGCCGTCGCCTCGACGATCTGCTCAAGCGCCGCGACAACGGCAAGACGACGTGGCTGGCCTGGCTGCGGCAATCCCCG
GTCAAACCGAACTCGCGGCACATGCTGGAACACATCGAACGCCTCAAGGCGTGGCAGGCGCTCGACCTGCCCTCCGGCATCGAGCGGCTGGTTCACCAGA
ACCGGCTGCTCAAGATCGCCCGCGAGGGCGGCCAGATGACGCCCGCCGACCTGGCGAAGTTCGAGCCGCAGCGGCGTTACGCGACCCTGGTGGCGCTCGC
CATCGAGGGCATGGCCACCGTCACCGACGAAATCATCGACCTGCATGACCGCATCCTGGGCAAGCTGTTCAATGCCGCCAAGAACAAGCATCAGCAGCAG
TTCCAGGCATCCGGCAAGGCGATCAATGCCAAGGTGCGGCTGTTCGGGCGCATCGGCCAGGCGCTGATCGAGGCCAAGCAAGCGGGCCGCGATCCGTTCG
CTGCCATCGAGGCCGTCATGTCCTGGGATGCTTTCGCCGAGAGCGTCACCGAAGCGCAGCGGCTCGCGCAACCCGAGGACTTCGATTTCCTGCACCGCAT
CGGCGAGAGCTACGCCACGCTGCGCCGCTACGCGCCGGAATTTCTCGACGTGCTCAAGTTGCGGGCCGCGCCCGCCGCCAAGGACGTACTCGACGCCATC
GAGGTGCTGCGCAGCATGAACAGCGACAACGCCCGCAAGGTGCCCACCGACGCGCCGACCGAGTTCATCAAGCCGCGCTGGCAGAAGCTGGTGATGACCG
ACACCGGCATCGACCGGCGCTACTACGAACTGTGCGCGCTGTCGGAGCTGAAGAACGCGCTGCGCTCCGGCGACATCTGGGTGCAAGGCTCGCGCCAGTT
CAAGGACTTCGAGGACTACCTGGTGCCGCCCGCGAAATTCGCCAGCCTCAAGCAGGCCAGCGAATTGCCGCTGGCCGTGGCCACCGATTGCGACCAGTAC
CTGCATGACCGGCTGACGCTGCTGGAAACGCAGCTCCCCACCGTCAACCGCATGGCGCTGGCCAACGAGCTGCCGGACGCCATCATCACGGAGTCGGGCC
TGAAGATCACGCCGCTCGATGCGGCGGTGCCCGATACCGCACAGGCCCTGATCGACCAGACGGCGATGATCCTACCGCACGTCAAGATCACCGAATTGCT
GCTGGAGGTAGACGAATGGACGGGCTTCACCCGGCACTTCGCCCACCTGAAGTCAGGCGACCTGGCCAAGGACAAAAACCTGTTGCTGACCACGATCCTC
GCCGACGCGATCAACCTGGGCCTGACCAAGATGGCGGAATCGTGCCCCGGCACGACCTACGCCAAGCTGGCCTGGCTGCAAGCCTGGCACATCCGCGACG
AAACCTACGGGGCGGCACTGGCCGAGCTGGTCAACGCGCAGTTCCGACATCCCTTCGCCGAGCATTGGGGCGACGGCACCACGTCATCGTCGGACGGCCA
GAACTTCCGCACCGGCAGCAAGGCCGAGAGCACCGGCCACATCAATCCGAAATACGGCAGCAGCCCAGGGCGGACGTTCTACACCCATATCTCCGACCAG
TACGCGCCGTTCCACACCAAGGTCGTGAACGTCGGCGTGCGCGACTCGACCTACGTGCTCGACGGCCTGCTGTATCACGAATCCGACCTGCGGATCGAGG
AGCACTACACCGACACGGCAGGGTTCACGGACCACGTCTTCGCGTTGATGCACCTGCTGGGCTTCCGCTTCGCCCCGCGCATTCGTGACCTGGGCGACAC
CAAGCTCTACATCCCGAAGGGCGATGCCACCTACGAGGCGTTGAAACCGATGATCGGCGGCACGCTCAACATCAAGCACGTCCGCGCCCATTGGGATGAA
ATCCTGCGGATGGCCACCTCGATCAAGCAGGGCACGGCGACGGCCTCGCTGATGCTCAGGAAGCTTGGCAGCTACCCGCGCCAGAACGGCCTGGCCGTCG
CCCTGCGTGAGCTGGGACGCATCGAGCGCACACTGTTCATCCTGGACTGGCTGCAAAGCGTCGAGCTGCGCCGCCGCGTGCATGCCGGGCTGAACAAAGG
CGAGGCGCGCAACGCGCTGGCCCGCGCCGTGTTCTTCAACCGCCTGGGGGAAATCCGCGACCGCAGCTTCGAGCAGCAGCGCTACCGGGCCAGCGGCCTC
AACCTGGTGACGGCGGCCATCGTGCTATGGAACACGGTCTATCTGGAGCGGGCCGCGAACGCCTTGCGTGGCCACGGTCAAGCCGTCGATGACGGCCTGT
TGCAGTACCTGTCGCCGCTCGGCTGGGAGCACATCAACCTGACCGGCGATTACCTCTGGCGCAGCAGCGCCAAGATCGGCGCGGGCAAGTTCAGGCCGCT
ACGGCCGCTGCAACCGGCTTAGCGTGCTTTATTTTCCGTTTTCTGAGACGACCCC

Protein section

ORF number : 8

ORF 1

Length		Begin	End	Strand	Fusion ORF
558 bp	185 aa	591	34	-	No

ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MLIGYARVSTQDQNLELQREALSKAGCKKVFEDKVSGTRADRPGLAKTLEMLREGDTLVVWKLDRLGRSVKQLVDLVGDLHKHGVQFRSLTDSIDTGTPS
GRFFFHVMASLAEMERELTVERTRAGLEVAKQLGRKGGRKPKMTDSKIESAKKLLASGVPPKDVAKNLGVSIPTLYRWVPASTHA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
372 bp	123 aa	956	585	-	No

ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MSTSTIEALASAWARIAEEAEFPADYEGTATPQAHRASEAIQEQIRERIVATNDMRLFSLLHLLGQASLRMEQALWPEDYERMTREVEEALRQATDANAR
SYTHEEVMQAMQERIDRARDKPC

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
501 bp	166 aa	1453	953	-	No

ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MKLSFHQHIALFWMIGAPGVFAPVIENAKRPDAGAVMAWGVAIVAVMILFTPLLLRCPPFRRWYGRTDALSERQRQALAERGLRRYYQTAFDDGYVPRVM
PYVWRIIWTVGGLMAVTAVLPTNTGRPAFDALVVFSTWYPIGVMLLVFASRPLGRLIRQRAQERRK

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
327 bp	108 aa	1776	1450	-	No

ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MSRSRRKTPIVGHTTCGSEREDKKLWHQRWRTRERTALTSASPEALSAHLPLLENQASSVWSMGKDGRSYWPVKRQAATADRIANHKGRNPQERASLKKR
LLRKWMSK

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
375 bp	124 aa	2405	2031	-	No

ORF function : Passenger Gene
Annotation : cupin 2 conserved barrel domain proteinDescription :

ORF sequence :

MKEGSPMTVESRIFSVAEYVQPSEGEPIRSVVLETRDSIIVVWHVHPGQEIAAHIHPHGQDTWTVLSGMADYFQGNGIVRALREGEIAVARPGQVHGARN
TGTEPFVFVSVVASANAGFVLAER

Blast result :

ORF 6

Length		Begin	End	Strand	Fusion ORF
387 bp	128 aa	3010	2624	-	No

ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MSENRLPDYLDHIQQAATDARSFVEGMAKDDFLADKRTQQAVIMSLIVIGEAATKVMDGYVEFTQAHADVPWRSMRNMRNRMAHGYFDINLDVVWETVQE
WLPALLQQLPAVRQDADDEDRNDKGMEP

Blast result :

ORF 7

Length		Begin	End	Strand	Fusion ORF
291 bp	96 aa	3297	3007	-	No

ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MRPSVVLDMKRSAVREAVGRFRAANPRVFGSVLHGTDRDGSDLDLLVDALPGATLLDLGDLEEELKSLLGVDVDLLTPGDLPPKFRAKVLAEAQPI

Blast result :

ORF 8

Length		Begin	End	Strand	Fusion ORF
2967 bp	988 aa	3456	6422	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRRSILSAAERESLLALPDTKDELIRHYTFSESDLSIIRQRRGPANRLGFAVQLCYLRFPGVILGADEPPFPPLLRLVANQLKVGIESWDEYGQREQTR
REHLVELQTVFGFQPFTIGHYRQAVQLLTELAMQTDKGIVLARALIEHLRRQSVIVPALNAVERASAEAITRANRRLYDALAEPLTDVHRRRLDDLLKRR
DNGKTTWLAWLRQSPVKPNSRHMLEHIERLKAWQALDLPSGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALAIEGMATVTDEIIDLHDRI
LGKLFNAAKNKHQQQFQASGKAINAKVRLFGRIGQALIEAKQAGRDPFAAIEAVMSWDAFAESVTEAQRLAQPEDFDFLHRIGESYATLRRYAPEFLDVL
KLRAAPAAKDVLDAIEVLRSMNSDNARKVPTDAPTEFIKPRWQKLVMTDTGIDRRYYELCALSELKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQ
ASELPLAVATDCDQYLHDRLTLLETQLPTVNRMALANELPDAIITESGLKITPLDAAVPDTAQALIDQTAMILPHVKITELLLEVDEWTGFTRHFAHLKS
GDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHIRDETYGAALAELVNAQFRHPFAEHWGDGTTSSSDGQNFRTGSKAESTGHINPKY
GSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDATYEALKPMI
GGTLNIKHVRAHWDEILRMATSIKQGTATASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAANALRGHGQAVDDGLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA

Blast result :

Comments

The transposase (ORF8) is 90% aa similar to ISSod9.
The others ORF :
ORF1 : DNA-invertase,
ORF2/3/4/6/7 : hypothetical protein,
ORF5 : cupin 2 conserved barrel domain protein

References

1] ISfinder annotation (2011)
2] Martin Holm Rau (2011) Direct submission to ISfinder.