ISCbo2
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_015425 | ND | Clostridium botulinum | Clostridium botulinum BKT015925 Clostridium botulinum BKT015925 plasmid p3BKT015925 Clostridium botulinum BKT015925 plasmid p1BKT015925 Clostridium botulinum BKT015925 plasmid p2BKT015925 |
DNA section
IS Length : 2198 bp
Ends
IR Length : 31/48
IRL : TATAAGTGCTATTATGGGAATGCAAGAAAGTGATCATTTAAGGATGTAAG
IRR : TATAAACGTCAAGATAATAATGAAATTTTCCGATCATTTAAGGGTGTAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTGCTTTCT | TTGTA | ATTTATTTGA | 5 |
| TTTACTGAGG | ACCATAGAAG | 0 | |
| GTAATCTTTC | ATTCC | ATATATCTGC | 5 |
| CCGAAAGTTA | TTGCAG | TATATGTGGC | 6 |
| TTCTACTTCC | ACAAGGCGACCTCTATATTGAGTTGTCTGCTTTCTTTGTA | ATTTATTTGA | 40 |
| TAATGTTACG | TCCGTT | TATAATTAAA | 6 |
| CACACCACCG | TA | TATGCTAAAA | 2 |
| TATACCTACT | ACATT | TGCTTTGTTT | 5 |
| CAGCATGGTA | AATG | TTATATATCT | 4 |
| ATCAGTATAA | ACCTCCC | ATTAAATTAC | 7 |
| AAATCTCATA | AAACT | AGCCTTTTTA | 5 |
| AGACCTATAC | ATAACTGGCG | 0 |
DNA sequence
TATAAGTGCTATTATGGGAATGCAAGAAAGTGATCATTTAAGGATGTAAGAATTAGATTTATGTATAATACTATAATTACCAACATTTAAACTGGAGGTA
ATTATGATTATACAAATGAATATTAATTCTGAAATACAAATAGATAAGTTAGAGGATCTTCATAAATTAAATTTAATTATGGAGGAAAATAATTTGAAAG
TAAATAAAAGTCAAATAGCTAGAGAACTTGGCGTTGATCCACGCACCGTAGGTAAATATTTAAATGGATATGTAAAACCTACTACTAGAAATCGTAAATC
TAAAATAGACCATTTTGAACCTATTATCAAAAAACTTCTTGGTAAAGATTCTATTCAAATATTTTATTATAAACGGATTCTGTGGCAGTATCTTAGAGAT
AACTATGGATTAGATTGTGCTCAATCTTCTTTTAGAAGGTATATCTCCAATCATCCAGAATTTAGTCACTATTTTAATAGCAGAAGAAAAGGACATCTAT
CGAAGGCTGCGCCTATGAGGTATGAAACAGGTAAAGGTAAACAAGCACAACTAGATTGGAAAGAAAACATAGAATTTGTTTTAAATACCGGAGAAATTAT
TAGTGTTAATGTCTTTGTATTAATACTTTCATATTCGAGGTTTAGAGTGTACAAGTTATCTCTCGATAAAACACAAGAAGTGTTATTTTCTTTTCTTGAT
GAATCATTTCAAGCTTTTGGAGGAGTTCCGGAGGAACTATTAACGGACAATATGAAAACTGTTATGGATTTACCTAGAACTAATTATTCTAAAGGAAAAG
TAAATAATAAATTTCAACAGTTTGCAAAAGATTACGGCTTTAAAGTTCACCCTTGCGTAGCAGGTAGACCTAACACCAAAGCCAAAGTAGAAGCTCCTAT
GAAATTATTAGATGAAATAAGAGCATATAATGGAACATTAAACTATGAGCAATTACACAAACTTGTTTCAGATTTAAACAATAGAATTAATAGTAAATGT
CATACATCAACAGGTAAAATACCAATATTACATTTACAGAAAGAAAAAGATTTCTTATCAAAACTGCCTAAAGATCAGATAAGAAATCTTTACAAAATAA
CCACCACATCTGTTAAAGTAAATAGTCAAAGCATGATTTCATACAAATCAAACCAATATTCTGTCCCACCAGAATATATAGGTAAACGACTAAAACTTCA
AGTATATGATCATCAATTACATGTGTATTATAGCACAAAATTAGTTACTATTCATGATATACAAAATCAAAAATTAAATTATCATGCAGAACATTATGCT
GAGATTAGTGCTTTAACATTTAATAAAAGCTCATATGAAATGATGAAGAAAGCTAGGACAAAATTTAAATTTAATAGGAGAGGTATATAAAAATGAATAG
TGCATATACACAACTTATAAAAAATCTAGAGTATTTAAAATTTAAACAAATGATTAATCATTTAGACGAAGTCATTGATTTTTCTACTAAAAATAATTTA
TCCTTTGTTGATGCTCTTATTAAGCTTACAGCTTATGAAATAGATTTTAAAGAAGCAAATATGATTAAATCTATGGTAAAAGTAGGCGCTTTTCCTCATA
AAAAAGAGGTTAAAGACTTTGATTTCAGCTTTCAACCTAGCATTAATAAAGATCAGATATTAGATTTTTTAACATTACGCTTTCTAAATACACAAGAAAA
TATAGTTTTCCTAGGTCCTAGTGGAGTAGGAAAAACGCACCTTGCTACATCTATAGGAATTGCGGCAGCAAAACGTAGATATAGTACATACTTTATTAAA
TGTCATGATTTATTACAGCAATTAAAACGTGCAAATTTAGAGAATCGATTAGATTCTAGACTTAAACATTTTAGTAAGTACAAGCTCCTAATAATAGATG
AATTAGGCTATCTACCGATAAATAAAGAAGACTCTAAGTTATTCTTCCAACTCATTGACATGCGATATGAGAAAAAAAGTACAATTTTAACAACTAATAT
AAATTTCAATGCTTGGGATGATATTTTTTATGATCCTATCATCGCAAATGCTATATTAGATAGAGTTTTGCACCATGCTCATGTTGTACCTATTAATGGA
AAGTCTTATCGCTTAAAAGATCACTTTAAAGATGACGATGAGTAAAAAACTACACCCTTAAATGATCGGAAAATTTCATTATTATCTTGACGTTTATA
ATTATGATTATACAAATGAATATTAATTCTGAAATACAAATAGATAAGTTAGAGGATCTTCATAAATTAAATTTAATTATGGAGGAAAATAATTTGAAAG
TAAATAAAAGTCAAATAGCTAGAGAACTTGGCGTTGATCCACGCACCGTAGGTAAATATTTAAATGGATATGTAAAACCTACTACTAGAAATCGTAAATC
TAAAATAGACCATTTTGAACCTATTATCAAAAAACTTCTTGGTAAAGATTCTATTCAAATATTTTATTATAAACGGATTCTGTGGCAGTATCTTAGAGAT
AACTATGGATTAGATTGTGCTCAATCTTCTTTTAGAAGGTATATCTCCAATCATCCAGAATTTAGTCACTATTTTAATAGCAGAAGAAAAGGACATCTAT
CGAAGGCTGCGCCTATGAGGTATGAAACAGGTAAAGGTAAACAAGCACAACTAGATTGGAAAGAAAACATAGAATTTGTTTTAAATACCGGAGAAATTAT
TAGTGTTAATGTCTTTGTATTAATACTTTCATATTCGAGGTTTAGAGTGTACAAGTTATCTCTCGATAAAACACAAGAAGTGTTATTTTCTTTTCTTGAT
GAATCATTTCAAGCTTTTGGAGGAGTTCCGGAGGAACTATTAACGGACAATATGAAAACTGTTATGGATTTACCTAGAACTAATTATTCTAAAGGAAAAG
TAAATAATAAATTTCAACAGTTTGCAAAAGATTACGGCTTTAAAGTTCACCCTTGCGTAGCAGGTAGACCTAACACCAAAGCCAAAGTAGAAGCTCCTAT
GAAATTATTAGATGAAATAAGAGCATATAATGGAACATTAAACTATGAGCAATTACACAAACTTGTTTCAGATTTAAACAATAGAATTAATAGTAAATGT
CATACATCAACAGGTAAAATACCAATATTACATTTACAGAAAGAAAAAGATTTCTTATCAAAACTGCCTAAAGATCAGATAAGAAATCTTTACAAAATAA
CCACCACATCTGTTAAAGTAAATAGTCAAAGCATGATTTCATACAAATCAAACCAATATTCTGTCCCACCAGAATATATAGGTAAACGACTAAAACTTCA
AGTATATGATCATCAATTACATGTGTATTATAGCACAAAATTAGTTACTATTCATGATATACAAAATCAAAAATTAAATTATCATGCAGAACATTATGCT
GAGATTAGTGCTTTAACATTTAATAAAAGCTCATATGAAATGATGAAGAAAGCTAGGACAAAATTTAAATTTAATAGGAGAGGTATATAAAAATGAATAG
TGCATATACACAACTTATAAAAAATCTAGAGTATTTAAAATTTAAACAAATGATTAATCATTTAGACGAAGTCATTGATTTTTCTACTAAAAATAATTTA
TCCTTTGTTGATGCTCTTATTAAGCTTACAGCTTATGAAATAGATTTTAAAGAAGCAAATATGATTAAATCTATGGTAAAAGTAGGCGCTTTTCCTCATA
AAAAAGAGGTTAAAGACTTTGATTTCAGCTTTCAACCTAGCATTAATAAAGATCAGATATTAGATTTTTTAACATTACGCTTTCTAAATACACAAGAAAA
TATAGTTTTCCTAGGTCCTAGTGGAGTAGGAAAAACGCACCTTGCTACATCTATAGGAATTGCGGCAGCAAAACGTAGATATAGTACATACTTTATTAAA
TGTCATGATTTATTACAGCAATTAAAACGTGCAAATTTAGAGAATCGATTAGATTCTAGACTTAAACATTTTAGTAAGTACAAGCTCCTAATAATAGATG
AATTAGGCTATCTACCGATAAATAAAGAAGACTCTAAGTTATTCTTCCAACTCATTGACATGCGATATGAGAAAAAAAGTACAATTTTAACAACTAATAT
AAATTTCAATGCTTGGGATGATATTTTTTATGATCCTATCATCGCAAATGCTATATTAGATAGAGTTTTGCACCATGCTCATGTTGTACCTATTAATGGA
AAGTCTTATCGCTTAAAAGATCACTTTAAAGATGACGATGAGTAAAAAACTACACCCTTAAATGATCGGAAAATTTCATTATTATCTTGACGTTTATA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1287 bp | 428 aa | 104 | 1390 | + | No |
Chemistry : DDE
ORF sequence :
MIIQMNINSEIQIDKLEDLHKLNLIMEENNLKVNKSQIARELGVDPRTVGKYLNGYVKPTTRNRKSKIDHFEPIIKKLLGKDSIQIFYYKRILWQYLRDN
YGLDCAQSSFRRYISNHPEFSHYFNSRRKGHLSKAAPMRYETGKGKQAQLDWKENIEFVLNTGEIISVNVFVLILSYSRFRVYKLSLDKTQEVLFSFLDE
SFQAFGGVPEELLTDNMKTVMDLPRTNYSKGKVNNKFQQFAKDYGFKVHPCVAGRPNTKAKVEAPMKLLDEIRAYNGTLNYEQLHKLVSDLNNRINSKCH
TSTGKIPILHLQKEKDFLSKLPKDQIRNLYKITTTSVKVNSQSMISYKSNQYSVPPEYIGKRLKLQVYDHQLHVYYSTKLVTIHDIQNQKLNYHAEHYAE
ISALTFNKSSYEMMKKARTKFKFNRRGI
YGLDCAQSSFRRYISNHPEFSHYFNSRRKGHLSKAAPMRYETGKGKQAQLDWKENIEFVLNTGEIISVNVFVLILSYSRFRVYKLSLDKTQEVLFSFLDE
SFQAFGGVPEELLTDNMKTVMDLPRTNYSKGKVNNKFQQFAKDYGFKVHPCVAGRPNTKAKVEAPMKLLDEIRAYNGTLNYEQLHKLVSDLNNRINSKCH
TSTGKIPILHLQKEKDFLSKLPKDQIRNLYKITTTSVKVNSQSMISYKSNQYSVPPEYIGKRLKLQVYDHQLHVYYSTKLVTIHDIQNQKLNYHAEHYAE
ISALTFNKSSYEMMKKARTKFKFNRRGI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 753 bp | 250 aa | 1393 | 2145 | + | No |
AG : IS21 helper
ORF sequence :
MNSAYTQLIKNLEYLKFKQMINHLDEVIDFSTKNNLSFVDALIKLTAYEIDFKEANMIKSMVKVGAFPHKKEVKDFDFSFQPSINKDQILDFLTLRFLNT
QENIVFLGPSGVGKTHLATSIGIAAAKRRYSTYFIKCHDLLQQLKRANLENRLDSRLKHFSKYKLLIIDELGYLPINKEDSKLFFQLIDMRYEKKSTILT
TNINFNAWDDIFYDPIIANAILDRVLHHAHVVPINGKSYRLKDHFKDDDE
QENIVFLGPSGVGKTHLATSIGIAAAKRRYSTYFIKCHDLLQQLKRANLENRLDSRLKHFSKYKLLIIDELGYLPINKEDSKLFFQLIDMRYEKKSTILT
TNINFNAWDDIFYDPIIANAILDRVLHHAHVVPINGKSYRLKDHFKDDDE
Blast result :
Comments
ISCbo2 is 70% (ORFA : the transposase ) and 86% (ORFB : the helper of transposition) aa similar to IS232A.
Atypical ends defined by empty site. The ISCbo2 sequence is inserted into a RNA-directed DNA polymerase gene.
Atypical ends defined by empty site. The ISCbo2 sequence is inserted into a RNA-directed DNA polymerase gene.
References
1] Hanna Skarin (2011) Direct submission
2] ISfinder annotation (2011)
3] Skarin,H., Hafstrom,T., Westerberg,J. and Segerman,B.(2011) BMC Genomics 12 (1), 185
2] ISfinder annotation (2011)
3] Skarin,H., Hafstrom,T., Westerberg,J. and Segerman,B.(2011) BMC Genomics 12 (1), 185