ISPve1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
HQ384165 Y Paracoccus versutus
Paracoccus bengalensis DSM 17099
Paracoccus versutus UW1
DNA section
IS Length : 2503 bp

Ends


IR Length : 17/28

IRL : TGAGAATGGCGGTGCGAAATTAGTCCACGTTAGCGGCGGCATGGTGCTGC
IRR : TGTCAACGGCGGCGTAAAAACCTGCCACTGGGCGGCGCAAAAGTCGGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CGTGTTGACTATTTTACCTCTGGCGGTG8

DNA sequence

TGAGAATGGCGGTGCGAAATTAGTCCACGTTAGCGGCGGCATGGTGCTGCTGCGGGCGGCGTAAAAGTCGTCCACCTTTTCCCTTTCTGCGGCTGCAGGG
AGGATGGGAGGATCTACAGCGTGGAACTCTATCTCAAGGTTCGTCAGGCCTGTGCTGCTGGTATGAGCCAGCGTCAGGCGGCGACGACATTCAACATATC
GCGCGACACGGTCGCAAAGATGATGACGTTCTCGGTTCCTCCGGGCTACCGGCGGACGGCCGAGGTCAGGCGCCCGAAGCTCGATCCGTTCATCCCGATC
ATCGAGGGTTGGCTGGAGGCGGACCGGACCATGCCGCGCAAGCAGCGGCATACGGCGAAGCGTGTGTTTGACCGTCTGCGCGAAGAATGCGGTTTCACCG
GCGGCTACACGATCATCAAGGATTATATCCGGGAACGTGAGCGGCGGGGCCAGGAGGTGTTCGTGCCGCTGGCGCATCCCCCAGGACACGCGCAGGCCGA
CTTCGGCGAGGCAATGGTTCGGATCGGAGGTGTCGAACAGAAGGCGCATTTCTTCGTGCTGGACTTGCCGCATAGCGACGCCTGCTACGTCCGTGCCTAT
CCGGCGGCGGTTGCCGAGGCCTGGGTGGACGGTCATGTCCATGCCTTTGCCTTTTTGGGGGCGGTTCCGCAGTCGATCGTCTACGACAATGACCGCTGCC
TGGTGGCGAAGATCCTGCCGGATGGCACCCGCAAGCGAGCCGCCCTGTTCAGCGGGTTTCTGTCGCATTACCTGATCCGGGATCGCTATGGCCGCCCTGG
CAAGGGGAACGACAAAGGCAATGTCGAAGGCCTGGTGGGCTATTGCCGCCGCAACTTCATGGTGCCGATGCCTGAGTTCGCCAGTTGGGAAGAATTCAAC
CTCTGGCTGGAGGAGCGCTGCAAGCGGCGTCAGCACGATGTCCTTCGCGGCGAGAGCGAAACGATCGGCGAGCGACTGCAGCGCGATCTCGCCGCGATGC
GCCCGCTCCCGGCGGCGCCCTTTGATGCCTGCGATCAGGCGACCGGCCGCGTGTCGTCGCAATCGCTGGTGCGCTACAAGACCAACGATTACTCGGTGCC
CGTCGCCTACGGCCATCAGGATGTCTGGATCCGGGGCTATATCCATGAGGTGGTGATCGGCTGCCGGGGCGAAGTGATCGCCCGCCATCCCCGGTGCTGG
GATCGCGACGAGATCGTCTTCGACCCGCTCCATTACCTTCCGCTGATCGAGCAGAAGATCAATGCGTTGGATCAGGCGGCACCATTGCAGGACTGGGACC
TGCCAGAGGAGTTCGCCACCCTGCGCCGGCTGATGGAGGCACGGATGAACCGGCACGGCCGACGCGAATATGTCCAGGTCCTGCGGCTTCTGGAAACCTT
CGATCTGGCCGATCTGCATGCGGCCGTGAGGCAGGCGCTGCAGATGGGGGCGATCGGCTTCGATGCGGTGAAGCATCTCTTGTTGTGCCGGGTGGAGCGC
CGGCCACCCCGGCTGGATCTGGACTGCTATCCCTACCTGCCCAGGGCAACGGTCGAGAAGACCGAGGCCCGCTCCTACATGCGGCTGCTGTCTGTCAATG
CGGAGGGCGTGGCATGAGCGACGCCCCGGAAATCCTGCTTGCCCATCACCTCAAGGCGCTGAAACTGCCGACCTTCCTGCGAGAACACCAGAAACTGGCC
CGGCAATGTGCGGCGGAAGGCGTGGACCATGTCCGATATCTCGCCCGGCTCGTCGAACTGGAACTGATCGACCGGGAACGGCGGATGGTCGAGCGGCGCA
TCAAGGCCGCCAGGTTCCCGGCCGTCAAAAGCCTGGACAGCTTCGACTTCACGGTCATCCCGAAGCTCAACAAGATGCAGGTGCTGGAACTGGCCCGGTG
CGAATGGATCGAGCGGCGCGAGAACATTATTGCTCTCGGCCCCAGTGGCACGGGCAAGACGCACATCTCGCTCGGCCTTGGTCTGGCCGCCTGCCAGAAG
GGCCTGTCCGTCGGCTTCACCACCGCCGCGGCCCTGGTCAGCGAGATGATGGAGGCCCGCGACGAGCGCCGCCTGCTGCGCTTCCAGAAGCAGATGGCAG
GATACAAGCTGCTCATCATCGATGAACTCGGCTTCGTGCCGCTGTCGAAGACCGGCGCCGAGCTGCTGTTCGAACTGATCTCGCAACGCTACGAGCGCGG
ATCGATCCTGATCACCAGCAATCTGCCTTTCGACGAATGGACCGAAACTTTCGGCTCCGAACGCCTCACCGGCGCGCTTCTCGACCGGCTGACCCACCAC
GTCAACATCCTCGAGATGAATGGCGAAAGCTACCGCCTCGCCAACAGTCGCGCCAGAAAGGCCGGCTGATCCCCCCTCCTCGAAAACCGCAACGCGCGGC
TGAGACCCCCGCTCGGGCTACGCCCTCGCAGCGGTCTCAGCCGCGCACAACGGTGGCCGACTTTTGCGCCGCCCAGTGGCAGGTTTTTACGCCGCCGTTG
ACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1455 bp484 aa1631617+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQRQAATTFNISRDTVAKMMTFSVPPGYRRTAEVRRPKLDPFIPIIEGWLEADRTMPRKQRHTAKRVFDRLREECGFTGGYTIIKDYIRERERRGQEVF
VPLAHPPGHAQADFGEAMVRIGGVEQKAHFFVLDLPHSDACYVRAYPAAVAEAWVDGHVHAFAFLGAVPQSIVYDNDRCLVAKILPDGTRKRAALFSGFL
SHYLIRDRYGRPGKGNDKGNVEGLVGYCRRNFMVPMPEFASWEEFNLWLEERCKRRQHDVLRGESETIGERLQRDLAAMRPLPAAPFDACDQATGRVSSQ
SLVRYKTNDYSVPVAYGHQDVWIRGYIHEVVIGCRGEVIARHPRCWDRDEIVFDPLHYLPLIEQKINALDQAAPLQDWDLPEEFATLRRLMEARMNRHGR
REYVQVLRLLETFDLADLHAAVRQALQMGAIGFDAVKHLLLCRVERRPPRLDLDCYPYLPRATVEKTEARSYMRLLSVNAEGVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa16142369+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSDAPEILLAHHLKALKLPTFLREHQKLARQCAAEGVDHVRYLARLVELELIDRERRMVERRIKAARFPAVKSLDSFDFTVIPKLNKMQVLELARCEWIE
RRENIIALGPSGTGKTHISLGLGLAACQKGLSVGFTTAAALVSEMMEARDERRLLRFQKQMAGYKLLIIDELGFVPLSKTGAELLFELISQRYERGSILI
TSNLPFDEWTETFGSERLTGALLDRLTHHVNILEMNGESYRLANSRARKAG

 

Blast result :
Comments
ISPve1 has been identified by its transposition into entrapment vector pMMB2.
ISPve1 is 89% (ORFA : the transposase) and 97% (ORFB : the helper of transposition) aa similar to ISSsp5.
References
1] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277