ISfinder Mirror Site

ISDsp5

Family IS3
Group IS407

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC-013552	ND	Dehalococcoides sp.	Dehalococcoides sp. GT Dehalococcoides sp. VS

DNA section

IS Length : 1210 bp

Ends

IR Length : 12/16

IRL : TGACCTGACCCCCTAAAACGAGTCCAGTTTTAATGTTAGACTGTGACCGA
IRR : TGACCTGCCCCCATTAGTTGTACCACTTGTTAAGTTAGTACAGCGCTCAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCTTATCAAAAACGTTTAC		GTGCCAGTCAGCATTTGCCTT	0
CTGGTTTTTA	TTGAT	TAATATCAAA	5
AAACGTTTAC		GTGCCAGTCA	0
TAGCTAGCAC		GTACCCCCTG	0
GTGCCAATAT		CTCGAAATAG	0
GACTATCTCA	AT	TCTCAATGTA	2
ATATATCCTG	GCAT	TGTTTAAGGA	4
GGGCTAAAGT		ACATCACAAG	0

DNA sequence

TGACCTGACCCCCTAAAACGAGTCCAGTTTTAATGTTAGACTGTGACCGAAAAGAAAGGGGACTAGATGCCTAGAAAAGCTTTTACCGCGGAACAGATCA
TCAACAAGCTTCGTGAAGCCGAGCTGATTCTGAATCAGGGCACAACGGTGCCTGAAGCCTGCCGAAAGATTGGAGTTACCGATCAGACCTACTATCGATG
GCGCAAGGAATATGGCGGCTTAAACGTTGACCAGGCCAAGCGCCTTAAGGACCTGGAAAAAGAAAACGTCCAGCTAAAGCGCCTTGTGGCCGATCTTTCA
CTCGATAAGGCCATATTGAAGGAGGCGTCCCGGGGAAACTCCTAAGCCCGTCCCAAAGGCGACAAGTGATAGCCAAAGTGCGTATCCGCCTTGGAATCTC
TGAAAGACGGGCTTGTCAGGTGCTGGGTCAAGCCAGATCAACACAGCGCCGTAAAGAATCGATTACCGACGACGAAGCGAAGCTTACGCTACGGATAATT
GAACTGGCCAGCAAATACGGACGATACGGGTATCGGAGAATCACGGCGATTTTGCAAATAGCTGGTTGGAGAGTGAATCACAAAAGAGTCGAACGGATCT
GGAGAAAGGAAGGGCTGAAGGTGCCCCAGAAGCAATCCAAGCGCGGTAGACTATGGCTAAATGATGGTTCCTGCATCAGGTTGCGCCCCGAGCATAAGGA
TCATGTTTGGAGCTACGATTTCGTGGCCGAGAGGACGATAAATGGCCGGGCGTTCCGGATATTGAATATCATTGATGAGTACACTCGCGAGTGCCTGGCG
ATGAAGATTGGCCGGCGGATCACCTCCCAGGAGGTCATTGATAAGCTGTTCGAGCTATTCCTCTTGAGAGGCATCCCGAAAAACATCAGGTCGGACAACG
GCCCGGAATTCACGGCCAAGCAAATCAGGAACTGGCTGAATAGGATCGGTGTAAATACTTTGTTCATTGAGCCGGGAAGCCCATGGGAGAACGGTTACAT
AGAATCATTCAACGGGAAACTGAGAGACGAACTGCTCAACCGGGAAATATTTTTTACCCTTACCGAGGCAAAAGTATTGATTGAAGAATGGAGGAAAGAC
TACAATCAAATACGCCCGCACAGCGCATTGGGTTACAGACCACCGGCTCCAGAGGCCGTAATGAGCGCTGTACTAACTTAACAAGTGGTACAACTAATGG
GGGCAGGTCA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
279 bp	92 aa	67	345	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
873 bp	290 aa	309	1181	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1115 bp	371 aa	67	1181	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

Blast result :

Comments

ISDsp5 is 95% aa similar to ISDsp2. The third ORf is a putative ORFAB transposase reconstructed in silico by possible -1 fraemshift.

References

1] ISfinder annotation (2011)
2] Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D., Goodwin,L., Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Kuske,C.R., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Ritalahti,K.M., Fletcher,K.E., Sung,Y., Wagner,D., Konstantinidis,K.T., Loeffler,F.E. and Woyke,T. (2010) Direct submission GenBank.