ISAzs32
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013856 | ND | Azospirillum sp. | Azospirillum sp. B510 plasmid pAB510b |
DNA section
IS Length : 1601 bp
Ends
IR Length : 9/10
IRL : gatggggTGATTGGGGTCACGCCGAGAAGGTGGCTGCTCCAGACGAGATG
IRR : ----ctcTGATTGGGTTTGTGGGGTCAAGGCGGGATGTGCCTTTTCTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAACTCCTTCTCGGACA | AGGGCTCTCAGAAGGCG | 0 | |
| AATAATCTTTCAGGACA | AAGACTCTAAGCCATCC | 0 |
DNA sequence
GATGGGGTGATTGGGGTCACGCCGAGAAGGTGGCTGCTCCAGACGAGATGAGGGAACGTGGTCCGTGTGCAACGGCCACAAGCATCACGGAGGAGCCGCC
ATGGAGTATTATGCCGGCCTGGACGTTTCGCTGGAAGAGACTGCGGTGTGCATCATCGATGCGCAGGGTGCGGTGGTGAAGGAGGCCAAGGTTGCCAGCG
AGCCGAAAGCGTTGATCGGCTTCTTTGAGGGGGTCGGCCTGTCGATGGGTCGGATCGGTCTGGAAGCGTGTTCGCTGACGGCTTGGCTTCACGATCGGAT
GGCGGCGGCGGGTCTGCCCGTCGTCTGCATGGAAACGCGCCAAGCCAAGGCGGCGATGGGGGCGATGCCGATCAAGACGGATCGCAACGATGCCCGCGGG
CTGGCGCAGATCGTGCGGACCGGCTGGTTTCGACCGGTGCACGTCAAGAGCGCGCAATGCCGGTCCTGGCGGGCGTTGCTGAGTGCCCGGCGCCTGGTGC
TGAACAAGTTGCGCGACATCGAAAACGGTGTGCGGGCGCTGCTGCGCGAGGTCGGGATCAAGCTCGGAACACCCGGACGCAAGCAATTCGCCGCCCGGGT
TCGCGAATTGGCGGCTTCCGACGCCGTCTTGGCGGCCTTGGCCGAACCGCTGCTGACGATCATCGAAACCATGCTGGGCGAACTGGCACGCCTGACCCGG
CAGGTTCTGACGATCGTACGCGGCGAAACGGTCTGCCGCCGCCTGATGACGGTGCCCGGCGTCGGCCCGCTGACTGCCTTGGCCTTCCGCGCCACGGTGG
ACCGGCCCGAGCGCTTCCGCCACTCGCGCGATGTCGGCGCCCATCTCGGCTTGACGCCGCGGCGCTACCAGTCAGGGGAAACCGATATCCAGGGAAGGAT
CAGCCGATGTGGCGACGAGCTGGCGCGAACCGCCCTGTATGAGGCGGCCCACGCGCTGCTCGTCCGCTCGCGGAAATGGTCGAGCCTGCGGGCCTGGGGC
ATGAAGGTCGCGAAGGCTCGCGGCATGGCCCGGGCACGCGTTGCCGTCGCCCGCAAGCTCGCGGTCATCCTGCATCGGATGTGGCAGGACGGCAGCGAGT
TCCGCTGGGGTAAGGAGCCCGTCGCCGCATGACGATGTAGAAGCCCACCAACCGAATTCGTCGGCAGCGACGAACCGGATCGTTCCCGTGGGGACGATGG
GCGAGGCGATCTCGTTGAGAGTCCGGAACCGCCGGGGGCAACTCCGGCAGGTCGCCAGACAGATTGAGACGCCTCGCCTTCCGGACCCCATGATGCGGCG
GCCTCCGCGCCGACCGCGAAGAGAAGCGCGTGACCCAGGGGAACGGTCATTCGTCCAGCCCGACCGCCGGCCAGTTCAGCACCGTGCTCGGGACCGTCAA
GGACGCGGTACCCGCGCCGCTGACGCGGTGACCTGCGGCCATCCTTGACCGTCCCGGCGCGCGGCGCTCTGACGATCCCAGGTCGGGACGGAGGGATGGT
CTGGATCAGTCGAACAAAGGGATAAGCACCCCCATACCCGCCGAAATCAGTAGGGAGCAGAAAAGGCACATCCCGCCTTGACCCCACAAACCCAATCAGA
G
ATGGAGTATTATGCCGGCCTGGACGTTTCGCTGGAAGAGACTGCGGTGTGCATCATCGATGCGCAGGGTGCGGTGGTGAAGGAGGCCAAGGTTGCCAGCG
AGCCGAAAGCGTTGATCGGCTTCTTTGAGGGGGTCGGCCTGTCGATGGGTCGGATCGGTCTGGAAGCGTGTTCGCTGACGGCTTGGCTTCACGATCGGAT
GGCGGCGGCGGGTCTGCCCGTCGTCTGCATGGAAACGCGCCAAGCCAAGGCGGCGATGGGGGCGATGCCGATCAAGACGGATCGCAACGATGCCCGCGGG
CTGGCGCAGATCGTGCGGACCGGCTGGTTTCGACCGGTGCACGTCAAGAGCGCGCAATGCCGGTCCTGGCGGGCGTTGCTGAGTGCCCGGCGCCTGGTGC
TGAACAAGTTGCGCGACATCGAAAACGGTGTGCGGGCGCTGCTGCGCGAGGTCGGGATCAAGCTCGGAACACCCGGACGCAAGCAATTCGCCGCCCGGGT
TCGCGAATTGGCGGCTTCCGACGCCGTCTTGGCGGCCTTGGCCGAACCGCTGCTGACGATCATCGAAACCATGCTGGGCGAACTGGCACGCCTGACCCGG
CAGGTTCTGACGATCGTACGCGGCGAAACGGTCTGCCGCCGCCTGATGACGGTGCCCGGCGTCGGCCCGCTGACTGCCTTGGCCTTCCGCGCCACGGTGG
ACCGGCCCGAGCGCTTCCGCCACTCGCGCGATGTCGGCGCCCATCTCGGCTTGACGCCGCGGCGCTACCAGTCAGGGGAAACCGATATCCAGGGAAGGAT
CAGCCGATGTGGCGACGAGCTGGCGCGAACCGCCCTGTATGAGGCGGCCCACGCGCTGCTCGTCCGCTCGCGGAAATGGTCGAGCCTGCGGGCCTGGGGC
ATGAAGGTCGCGAAGGCTCGCGGCATGGCCCGGGCACGCGTTGCCGTCGCCCGCAAGCTCGCGGTCATCCTGCATCGGATGTGGCAGGACGGCAGCGAGT
TCCGCTGGGGTAAGGAGCCCGTCGCCGCATGACGATGTAGAAGCCCACCAACCGAATTCGTCGGCAGCGACGAACCGGATCGTTCCCGTGGGGACGATGG
GCGAGGCGATCTCGTTGAGAGTCCGGAACCGCCGGGGGCAACTCCGGCAGGTCGCCAGACAGATTGAGACGCCTCGCCTTCCGGACCCCATGATGCGGCG
GCCTCCGCGCCGACCGCGAAGAGAAGCGCGTGACCCAGGGGAACGGTCATTCGTCCAGCCCGACCGCCGGCCAGTTCAGCACCGTGCTCGGGACCGTCAA
GGACGCGGTACCCGCGCCGCTGACGCGGTGACCTGCGGCCATCCTTGACCGTCCCGGCGCGCGGCGCTCTGACGATCCCAGGTCGGGACGGAGGGATGGT
CTGGATCAGTCGAACAAAGGGATAAGCACCCCCATACCCGCCGAAATCAGTAGGGAGCAGAAAAGGCACATCCCGCCTTGACCCCACAAACCCAATCAGA
G
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 101 | 1132 | + | No |
Chemistry : DEDD
ORF sequence :
MEYYAGLDVSLEETAVCIIDAQGAVVKEAKVASEPKALIGFFEGVGLSMGRIGLEACSLTAWLHDRMAAAGLPVVCMETRQAKAAMGAMPIKTDRNDARG
LAQIVRTGWFRPVHVKSAQCRSWRALLSARRLVLNKLRDIENGVRALLREVGIKLGTPGRKQFAARVRELAASDAVLAALAEPLLTIIETMLGELARLTR
QVLTIVRGETVCRRLMTVPGVGPLTALAFRATVDRPERFRHSRDVGAHLGLTPRRYQSGETDIQGRISRCGDELARTALYEAAHALLVRSRKWSSLRAWG
MKVAKARGMARARVAVARKLAVILHRMWQDGSEFRWGKEPVAA
LAQIVRTGWFRPVHVKSAQCRSWRALLSARRLVLNKLRDIENGVRALLREVGIKLGTPGRKQFAARVRELAASDAVLAALAEPLLTIIETMLGELARLTR
QVLTIVRGETVCRRLMTVPGVGPLTALAFRATVDRPERFRHSRDVGAHLGLTPRRYQSGETDIQGRISRCGDELARTALYEAAHALLVRSRKWSSLRAWG
MKVAKARGMARARVAVARKLAVILHRMWQDGSEFRWGKEPVAA
Blast result :
Comments
ISAzs32 is 84% aa similar to ISMno22.
References
1] ISfinder annotation (2010).
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.
2] Kaneko,T., Minamisawa,K., Isawa,T., Nakatsukasa,H., Mitsui,H., Kawaharada,Y., Nakamura,Y., Watanabe,A., Kawashima,K., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Sato,S. (2010) DNA Res. 17 (1), 37-50.