ISWpi10

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Wolbachia pipientis
Wolbachia pipientis
Wolbachia endosymbiont of Culex quinquefasciatus Pel
DNA section
IS Length : 1100 bp

Ends


IR Length : 15/20

IRL : TACCAATTCCCACTATGTAAAGAACAGATAGACAATAATGGGAAAGAAGT
IRR : TACCAAATTCCATTACTAATCGAACAAATAAGAAACATTACAAACTCGTT

Insertion site


Left flankDirect repeatRight flankDR Length
AATTTAAACGTAAGTATTCCAAA3
AGGGCTATACTGAGAGGTTGTCC3
GGACAACCTCTTACAATATCATC3
GGACAACCTCTTAGTTAATTTGT3
AAAAACGTCGTTATGAGAGGTTGTCC0
TATTTCATCGTCAGAGGTTGTCC3
GGACAACCTCTTACATAACCATA3
GGACAACCTCTTAGAGGTTGTCC3

DNA sequence

TACCAATTCCCACTATGTAAAGAACAGATAGACAATAATGGGAAAGAAGTGATACTAAAGTAGATAAAATAGAGAGGTATAAATGGCATTAAGGTCAAAA
CTGTTAGACGAAAAAGTTGTAAATTTGGCGAAAGAAATGTTAAAAAAGGTCAGAAATAACGCATATGTTTCAAAAAAGTTACAAGCGGTGATAGCAGGAA
AAGAAAGTAGTATAAGCGCTGTGGCAAGAATATGTAAAATTTCAAGGACTGCTTTGACTGAATGGATAAAGCATCTAAAATTTGGTAGAGTAGAAAAATT
ATTTTCCCCTTCTCAGCGGCGAAGAAAAAGCAAATTAAACAAAAATCAACGTGAGCAAATTGAAATATGGGTAGAAAGAAATCCAAATATTACTATTAAG
GAAGTGCAAATAAAAATCTCAGAGGAATTTGGCCTAAACATTAGCAAATCAACAGTGCATCGTGAGATACAAAGGATGAAGTTTTCTTACATAACACCGA
GGCCAATTCACCATAAACAAGATAAAAACAAGCAAGAAGAGTTTAAAAAATACTTCAATAAAATAGTCAATTCCCACCCTGAAAAGGAGGTATTTTTTTG
ATGAATCACGATTTGGAACTCATTCAAAAATCGGACATGGATGGTTTAAAAAAGGGGTCAGAACGCAGGTTAAAATGAAAATTGGTAGACAAAATTTCTA
TATCTACAGTGCGGTAAATCCAAGAAGTGGTAAGAAAATTAGCCTACTTGCTCCATATGTAAACACTGATTGTATGAATATATTTCTGGAGCAGATGTCG
AAAGATTTAGGCACGAAAAAAGCCTTTCTTGTAATGGATTGTGCAGGCTGGCATAGATCAAAAAGTTTGAAAATTCAGAAAAACATTACCATTATATACT
TGCCTCCTTATTCACCAGAACTGAATCCTGTTGAGAGGTTGTGGCAATATATCAAACACAATACTTTACGCAACAGAGTCTACGATACCATAGGCTTACT
TGAAGATGTTCTGTGTAATTTTATTGTCAGTATTTCCAGTGCTACTATTAAACGAGTTTGTAATGTTTCTTATTTGTTCGATTAGTAATGGAATTTGGTA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
519 bp172 aa83601+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MALRSKLLDEKVVNLAKEMLKKVRNNAYVSKKLQAVIAGKESSISAVARICKISRTALTEWIKHLKFGRVEKLFSPSQRRRKSKLNKNQREQIEIWVERN
PNITIKEVQIKISEEFGLNISKSTVHREIQRMKFSYITPRPIHHKQDKNKQEEFKKYFNKIVNSHPEKEVFF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
519 bp172 aa5671085+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SIPTLKRRYFFDESRFGTHSKIGHGWFKKGVRTQVKMKIGRQNFYIYSAVNPRSGKKISLLAPYVNTDCMNIFLEQMSKDLGTKKAFLVMDCAGWHRSKS
LKIQKNITIIYLPPYSPELNPVERLWQYIKHNTLRNRVYDTIGLLEDVLCNFIVSISSATIKRVCNVSYLFD

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1003 bp333 aa831085+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MALRSKLLDEKVVNLAKEMLKKVRNNAYVSKKLQAVIAGKESSISAVARICKISRTALTEWIKHLKFGRVEKLFSPSQRRRKSKLNKNQREQIEIWVERN
PNITIKEVQIKISEEFGLNISKSTVHREIQRMKFSYITPRPIHHKQDKNKQEEFKKYFNKIVNSHPEKEVFFDESRFGTHSKIGHGWFKKGVRTQVKMKI
GRQNFYIYSAVNPRSGKKISLLAPYVNTDCMNIFLEQMSKDLGTKKAFLVMDCAGWHRSKSLKIQKNITIIYLPPYSPELNPVERLWQYIKHNTLRNRVY
DTIGLLEDVLCNFIVSISSATIKRVCNVSYLFD

 

Blast result :
Comments
ISWpi10 transposase gene consists of two overlapping open reading frames presumably translated as a single protein via putative translational frameshifting.
Putative boundaries identified by aligning multiple copies with flanking sequences and identification of inverted repeats.
ISWpi10 is 52% aa similar to ISGdi5.
References
1] Cerveau N., and Cordaux R.(2010) Direct submission (manuscript in preparation)