ISfinder Mirror Site

ISWpi16

Family IS982
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Wolbachia pipientis	Wolbachia pipientis Wolbachia endosymbiont of Culex quinquefasciatus Pel

DNA section

IS Length : 981 bp

Ends

IR Length : 18

IRL : ACCTCAGTTATGGATTAGAAAATAGATAAAAGTATAACTAAGAAGAGGGA
IRR : ACCTCAGTTATGGATTAGCCGATAAAGAAAGGCATAGAAATAGAAGGCTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TAACTAATAT	AGATTA	GGTGATTTGG	6
CAGAAAACCT	TTACCA	ACTCTCACTA	6
TTATATTTGC	AGCGTA	AGGATTTTTT	6
AAACTTTCTA	TATAA	GATCTCTTCC	5
AGTCCAACAG	TTGAA	GTGCCAGAAG	5
CTTTTGTTAC	TAGTA	ATAACGAGGA	5
TTTCATATCC	TTGTA	TAGCTCTTCT	5
ATTGCTCACC	TTCTA	GGCTATCTGA	5
AAATATGCAG	TAGAA	GAGGAGCTGT	5
ACTTTTTTAT	TTGTG	GGTAAAAGTG	5
TAGGCAACAC	TAAAA	AAGTTCTCTT	5
ATCATGACTC	TCTAA	GGTGAACTGT	5
TCACAACCAT	TATGA	GTTGTTACAA	5
TGTTTGCTGC	TAAAA	GTAGTCATTT	5
GACAGAAAAC	TATAA	ATAACCCAAA	5
ATACTGCTTC	TTCTA	GTATATCAAC	5
GTATCGCCGC	TATGA	GCAAACAAGA	5
AATTTTATCC	TTTAG	GAGGGTTATG	5
ATTTTTTACC	TTTTA	GAAAACCTTT	5
AGACAGATAG	TAAAA	ATAGTGCTTA	5
GCTAACACAC	TACAG	AGAGTCGAAT	5
TTTCTAACTC	TTCGA	GGATGGAGCT	5
ATAACACCAT	CATAA	GGTGAACCAG	5
AAATCTTCTC	TTTAA	GTGAAATTAG	5
TAAAACTCAC	TACTG	GGGGATTCTT	5
GAATAAACTC	CACTA	CTGTATTTTG	5
TATTCTGTAC	ACATA	GTACATTCCC	5
ATGTGTGTAC	TAACA	ATACACACAT	5
CACATTCTTT	TAAAA	GAAGACAATT	5
GACATAGTAT	TACTA	ATGTTATAAA	5
TACTGCGCTA	TTA	CAGGGCCGGA	3
TAGGCCTTCA	TA	TAAAGAAAGA	2
TCACCACTGA	TA	AAAAGATGTT	2
AAGGCAGTTA	TA	TTAGACTTAT	2
ATTACTCTTC	CA	TTAGCCCCGA	2
CACACTAAAA		TGAAAAGACA	0
TTCCTTCAAA		TAGTAATACT	0
AGAACTTCTG		TTCTTGTACC	0
TACCTTTTTG		TAATGATAAT	0
AGCTCTCTCA		TTTGAGTACC	0
TTTTCTTACA		TATTAGACTT	0
AACACTATGA		TTTGAGGACT	0
CTCCTACCTA		TCATAATGTC	0
TCCGCTAGTA		CTCTAGGAAT	0

DNA sequence

ACCTCAGTTATGGATTAGAAAATAGATAAAAGTATAACTAAGAAGAGGGAAACAGGGTAAACTCGAGTATTTTAGTAATAATAAGAGGTTACCCATGAAG
AAAGATATTACAGAACTGTACTGTTGCGTCGAGGATTTTTGTCGTGCGGTAGATGATAATTTTGCAAATAGGTTCTTATCAAACGGCAAAAAACCAACCA
GAGTACCAGAAATAGCGCACTCAGAAATTCTAACCATAATCCTGTTATACCATAAATCACCATGTAAAAACTTCAAGGCTTTTTATCTTTGTTATCTTCA
GTTATTCTATAGATCAGAGTTTTCAAAGCTGCCTTCATATCACAGATTTATTGCCTTAAAGCCGCGAGTTTTGTGGTATTTAGCATTACTTTTGCAATGG
TTCTGTGAACAAGCGAAAATGACCGGCACTTCCTACATCGACACCTCTACGATTGCCGTTTGCCACAAAAAAAGGATCTCTAGGAATAAAGTTTTTAATC
ATCTAGCAGAAATTAGTAAGAGTACGTATGGTTGGTTTTTTGGCTTCAAGTTGCACTTAGTGATTAACGAAATTGGTGAAATTCAAGCACTTACCCTAAC
CAAAGGTAACGTTGATGACAGAAAACCTTTACCAACTCTCACTAAAAGACTGACCGGACTTTTGTTTGGAGATAAGGGCTATATAAAGAAAGAGCTTTTT
GAGAAACTCTTCGATAGAGGCCTAAAGTTAGTCACTAAAGTAAAAAAAGGTATGAAAAACGCACTAATTTCACTTAAAGAGAAGATTTTATTAAGGAAAA
GGTCGATTATTGAGACTGTTTTTGATTCCTTGAAAAACAAATTTGAGATTGAGCATACAAGACATAGATCGCCAACAAATTTCCTAATTCATGTTTTTTC
TGTTCTGATTTCCTACTCCATGCAGTCCAAAAAGCCTTCTATTTCTATGCCTTTCTTTATCGGCTAATCCATAACTGAGGT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
873 bp	290 aa	95	967	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKDITELYCCVEDFCRAVDDNFANRFLSNGKKPTRVPEIAHSEILTIILLYHKSPCKNFKAFYLCYLQLFYRSEFSKLPSYHRFIALKPRVLWYLALLL
QWFCEQAKMTGTSYIDTSTIAVCHKKRISRNKVFNHLAEISKSTYGWFFGFKLHLVINEIGEIQALTLTKGNVDDRKPLPTLTKRLTGLLFGDKGYIKKE
LFEKLFDRGLKLVTKVKKGMKNALISLKEKILLRKRSIIETVFDSLKNKFEIEHTRHRSPTNFLIHVFSVLISYSMQSKKPSISMPFFIG

Blast result :

Comments

Putative boundaries identified by aligning multiple copies with flanking sequences and identification of inverted repeats.
ISWpi16 is 67% aa similar to ISPasp1.

References

1] Cerveau N., and Cordaux R. (2010) Direct submission (manuscript in preparation)