ISWpi11

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Wolbachia pipientis
Wolbachia pipientis
Wolbachia endosymbiont of Culex quinquefasciatus Pel
DNA section
IS Length : 945 bp

Ends


IR Length : 22/23

IRL : GCTCTTTTCATAATAGTCCAAACGAGGTAAAATAAGTTTTTATAGTAGTG
IRR : GCTCTTTTCATAATAGTCCGAAAAGCTTATAGAAAAGCAGAATCAACAGC

Insertion site


Left flankDirect repeatRight flankDR Length
TTTAATATTATATAACTGCTTAAA4
TGTCTTCTATTATACTTGCTGATC4
AAAGCGTTAGTATAATTATCACGT4
AGACAATAGTTATACTTTCAACCA4

DNA sequence

GCTCTTTTCATAATAGTCCAAACGAGGTAAAATAAGTTTTTATAGTAGTGAAGGTAAGTATGCCAGCAGCATATAGTTATGATTTAAGAAAAAAAGCAAT
GGAAGTACTAGAAGAAGGGGAAAGGAGAGAAACAGTGGCAGCAAGATTTAAAATTGGAAAAACTACATTATGGGAGTGGCAGCAAAGAAAAAAAGAAACA
GGAGATTTTCAGTCAAAAAAACTTGGAAATGTAGGTTATAATCACAAAATTACCGACAGGAATAAATTTACTGAATTTGCCAAAAAACATGGTGGAAAAA
CTCTATCGGAAATGGCTAAGCTTTGGGGCAATGTTAGTATCACAACGATCCATCGAGCTCTTAAAAAAATTGGACTCACACGCAAAAAAAGACATATGGA
TACAAAGAAAGGAACGAAGAAAAACGCGCTGAATTTTTGAAAATTATAGCAACAAAAGAACCTCAAAACTTAGTATATATAGATGAGTCTGGCATTGATA
ACACTGAGGACTACCCTTATGGATATTGCCAGAAGGGACAGCGCTTTTATGCTCTAAAATCTGGTAAAAAAATTCAACGAATCAGTATGATTGCAGCCTT
AAACGGCAGAAAAATAGCTGTTCCGTTAACATTTGAAGGCCACTGTAATATGGATATCTTTAATAAATGGTTTGAGCAGTTCCTGACACCGATTTTGGAA
CCTGGACAAACTGTTATCCTTGATAATGCTACTTTTCATAAGTCTAATAAGATTACTGAACTTGCTAAAGGAGTTGGTGCAGAAATTTTATATCTACCGC
CTTATTCTCCCGATTTTAACAAAATTGAGCATCATTGGTTTGCTATAAAAAACAGAGCTAGGAAAAACATCCCTCTATTCACATCTTTTCGTCATGCTGT
TGATTCTGCTTTTCTATAAGCTTTTCGGACTATTATGAAAAGAGC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
381 bp126 aa60440+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPAAYSYDLRKKAMEVLEEGERRETVAARFKIGKTTLWEWQQRKKETGDFQSKKLGNVGYNHKITDRNKFTEFAKKHGGKTLSEMAKLWGNVSITTIHRA
LKKIGLTRKKRHMDTKKGTKKNALNF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
555 bp184 aa365919+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KNWTHTQKKTYGYKERNEEKRAEFLKIIATKEPQNLVYIDESGIDNTEDYPYGYCQKGQRFYALKSGKKIQRISMIAALNGRKIAVPLTFEGHCNMDIFN
KWFEQFLTPILEPGQTVILDNATFHKSNKITELAKGVGAEILYLPPYSPDFNKIEHHWFAIKNRARKNIPLFTSFRHAVDSAFL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
860 bp286 aa60919+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPAAYSYDLRKKAMEVLEEGERRETVAARFKIGKTTLWEWQQRKKETGDFQSKKLGNVGYNHKITDRNKFTEFAKKHGGKTLSEMAKLWGNVSITTIHRA
LKKIGLTRKKKTYGYKERNEEKRAEFLKIIATKEPQNLVYIDESGIDNTEDYPYGYCQKGQRFYALKSGKKIQRISMIAALNGRKIAVPLTFEGHCNMDI
FNKWFEQFLTPILEPGQTVILDNATFHKSNKITELAKGVGAEILYLPPYSPDFNKIEHHWFAIKNRARKNIPLFTSFRHAVDSAFL

 

Blast result :
Comments
ISWpi11 transposase gene consists of two overlapping open reading frames presumably translated as a single protein via putative translational frameshifting.
Putative boundaries identified by aligning multiple copies with flanking sequences and identification of inverted repeats.
ISWpi11 is 66% aa similar to ISAcma31.
References
1] Cerveau N., and Cordaux R. (2010) Direct submission (manuscript in preparation)