ISWpi4
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Wolbachia pipientis | Wolbachia pipientis Wolbachia sp. wRi |
DNA section
IS Length : 1011 bp
Ends
IR Length : 26/27
IRL : TGTATGGTCCCAGAGTGTGATGGTATGATAAAATATGAGAAAACAATACC
IRR : TGTATGGTCCCAGAGTGTGATGGTGTGGATTTAGTATGAACAATTCAGGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAGGGGGGGA | CTCCCTGTGT | 0 |
DNA sequence
TGTATGGTCCCAGAGTGTGATGGTATGATAAAATATGAGAAAACAATACCATCAAAGGAGGAATTATGGGACAAATATTACATGGGTGCGCCAAAACAAC
AGAGACAATTCGTAGAGCAATACAAAATAGTAAAGAGAGCATAGCAAAACTCTCCAGGTACTATAACCTTAATCCAAAAACTATCATTAAATGGAGAAAA
CGGTCTTTTGCCAAAGACGCTGATATGGGTCCGAAGCATGCAAGATCAACTGTTTTAACTTTAGAAGAGGAGGCTGTAATTGTTGCATTTCGTAAGCATA
CTCTATTGCCATTAGATGATTGTCTCTACGCTTTGCAAACCTCAATCCCACACTTGACTCGCTCTAGTCTACATCGTTGCTTACAGAGGCACAGCATTAG
CAGACTACCAGATGTTGATGGCGAAGTTAAACCAAAAAAGAAATTCAAACAATATCCTATAGGCTATTTCCATATTGATATTGCAGAAGTCAGAACAGAA
GAAGGAAAGTTATATTTGTTTGTTGCAATAGATAGAACATCCAAATTTACGTATGTTGAGTTGCATAAAAGTGCTACAAAACCTATAGCTGCTGAGTTTC
TACGTAATTTGATTAAAATTTTACCATATAAAATCCACACTATTTTGACAGATAACAGTGTACAATTTACCAACCAAAAACGCCATAAATACGCTTTTCA
ACATATATTTGATAGAGCTTGTGAAGAAAATGACATTGAACACCGTCTAACAAAAGTTAGCCATCCTTGGACAAATGGGCAAGTTGAGCGTATGAACAAA
ACTTTAAAGAATGCCACTGTTAAGAAGTATTACTATCAGTCTCACCAGCAACTCAAAGAGCATCTTTATGACTTTGTTATGGCCTACAATTATGCTAAAA
GACTCAAAACTCTTAAGGGTCTTACACCTTTTGAGTTTATATCTTTACAGTGGACAAAATCTCCTGAATTGTTCATACTAAATCCACACCATCACACTCT
GGGACCATACA
AGAGACAATTCGTAGAGCAATACAAAATAGTAAAGAGAGCATAGCAAAACTCTCCAGGTACTATAACCTTAATCCAAAAACTATCATTAAATGGAGAAAA
CGGTCTTTTGCCAAAGACGCTGATATGGGTCCGAAGCATGCAAGATCAACTGTTTTAACTTTAGAAGAGGAGGCTGTAATTGTTGCATTTCGTAAGCATA
CTCTATTGCCATTAGATGATTGTCTCTACGCTTTGCAAACCTCAATCCCACACTTGACTCGCTCTAGTCTACATCGTTGCTTACAGAGGCACAGCATTAG
CAGACTACCAGATGTTGATGGCGAAGTTAAACCAAAAAAGAAATTCAAACAATATCCTATAGGCTATTTCCATATTGATATTGCAGAAGTCAGAACAGAA
GAAGGAAAGTTATATTTGTTTGTTGCAATAGATAGAACATCCAAATTTACGTATGTTGAGTTGCATAAAAGTGCTACAAAACCTATAGCTGCTGAGTTTC
TACGTAATTTGATTAAAATTTTACCATATAAAATCCACACTATTTTGACAGATAACAGTGTACAATTTACCAACCAAAAACGCCATAAATACGCTTTTCA
ACATATATTTGATAGAGCTTGTGAAGAAAATGACATTGAACACCGTCTAACAAAAGTTAGCCATCCTTGGACAAATGGGCAAGTTGAGCGTATGAACAAA
ACTTTAAAGAATGCCACTGTTAAGAAGTATTACTATCAGTCTCACCAGCAACTCAAAGAGCATCTTTATGACTTTGTTATGGCCTACAATTATGCTAAAA
GACTCAAAACTCTTAAGGGTCTTACACCTTTTGAGTTTATATCTTTACAGTGGACAAAATCTCCTGAATTGTTCATACTAAATCCACACCATCACACTCT
GGGACCATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 946 bp | 315 aa | 66 | 1011 | + | No |
Chemistry : DDE
ORF sequence :
MGQILHGCAKTTETIRRAIQNSKESIAKLSRYYNLNPKTIIKWRKRSFAKDADMGPKHARSTVLTLEEEAVIVAFRKHTLLPLDDCLYALQTSIPHLTRS
SLHRCLQRHSISRLPDVDGEVKPKKKFKQYPIGYFHIDIAEVRTEEGKLYLFVAIDRTSKFTYVELHKSATKPIAAEFLRNLIKILPYKIHTILTDNSVQ
FTNQKRHKYAFQHIFDRACEENDIEHRLTKVSHPWTNGQVERMNKTLKNATVKKYYYQSHQQLKEHLYDFVMAYNYAKRLKTLKGLTPFEFISLQWTKSP
ELFILNPHHHTLGPY
SLHRCLQRHSISRLPDVDGEVKPKKKFKQYPIGYFHIDIAEVRTEEGKLYLFVAIDRTSKFTYVELHKSATKPIAAEFLRNLIKILPYKIHTILTDNSVQ
FTNQKRHKYAFQHIFDRACEENDIEHRLTKVSHPWTNGQVERMNKTLKNATVKKYYYQSHQQLKEHLYDFVMAYNYAKRLKTLKGLTPFEFISLQWTKSP
ELFILNPHHHTLGPY
Blast result :
Comments
Putative boundaries identified by detection of inverted repeats.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR. The transposase in the database is cut at the end of the IS.
An alternative start codon (Isoleucine) is localisated in position 51 on the same frame.
ISWpi4 is 82% aa similar to ISNe2.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR. The transposase in the database is cut at the end of the IS.
An alternative start codon (Isoleucine) is localisated in position 51 on the same frame.
ISWpi4 is 82% aa similar to ISNe2.
References
1] Cerveau N., and Cordaux R. (2010) Direct submission (manuscript in preparation)