ISVa5

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Vibrio anguillarum
Listonella anguillarum 775 plasmid pJM1
Vibrio anguillarum NB10
Listonella anguillarum plasmid pEIB1
DNA section
IS Length : 2381 bp

Ends


IR Length : 15/19

IRL : GTAAGCGCGCCATAAACCCCGCATTCTAATCTTTCAGCGCGAAGAATAAG
IRR : GTATCCGCCCCATGAACCCACGGGCCGACTTGTCAGTGTTTGAAGTTCCA

Insertion site


Left flankDirect repeatRight flankDR Length
TACCTTTAATAGAGAATGCG0
ATGTTGTATCCTTTGAAATT0

DNA sequence

GTAAGCGCGCCATAAACCCCGCATTCTAATCTTTCAGCGCGAAGAATAAGATCAACTCTCCAACCCAAAGGAGAGTTGAAATGGCAAAACGACGCACCAA
CCTAGAATGGCAATCACTGTTTGAACAATATGAAAGCAGCAGTGTTACCCAACGCGCTTTTTGCGAAGAGCATGGACTGAGTTTATCCACGTTCTTCGCC
AAGCGGCGTCAACTCCAAACAGTAAACCAATCGGAATTTGTCGGATTTGTTAAAGCCGAAATCGTCGAAAAGACAACCAAGTATCAGGCCCAGATAGCTA
CGGCTAACATGACACTTCTCATCAATGATGTGGAGCTGAGCATTCCTCAAGGTACGCCAGCGACCTATCTTGCTGAACTCATTGGAGCGTTGTCATGAAA
GGTATGCTCAGCGCTCCAGACATTTACCTTTATCGTGAAAGCGTCGATTTTAGAAAGTCCATCAACGGTCTTGCAGCGATTATCGAAAATGATACGGACT
TACCTCTTGGCAGCGGTGCGCTGTTCCTGTTTACCAATAAACAGCGCGATAAGATTAAGGTACTGTACTGGGATAAAACAGGCTTCGCCCTCTGGTATAA
ACGCCTTGAAAAAGCCAAGTATAAGTGGCCTTCAAAAGAGAAAAATCAGGTGTTTACCCTCACTCAATTTGAGCTTGATAGACTGCTTTCTGGCTTCACA
ATTATCGGCCATGAACCCGTAAAAATAAACGATTTTACAATGAGCTAATCGAGAATAAAGCCTTATATACCAAGCGTTAACGGCACGGTTTTAGTATAAT
CAATGCCATGGAAAAGACGCCAAATATCAACCCAGAAAGCCAAGATATTGCCGAGCTACAAGCGATGGTGAAAGCCCTGATGCTCTCGCAAGAGCAGAAG
GAATCGCAATGGCAACAAGAGCGACAATCGCTGCTTGAACAACTCAAACTCGCCTTCGACCGCCAGTTCGCTAAACGCTCGGAGGCGTTAAAGCCTTACG
ATGAATCACAAGGTGACCTCTTCAACGAAGCGGAATGTGAAGCCGTTAAGGAAGAAGAAGTTGAGGTGACAACCACGATCACAACGAAAAAGCGTGGTAA
ACGTAAACCTCTGCCTAAGACCTTACCTCGTGAGGTTATCGAACTCGATTTAGACGACCATGAAAAGCAGTGTTCTTGTTGTAATCATGGCCTGCATAAA
ATCGGTGAAGACCGCAGCGAGAAGCTAGAGTTCACGCCTGCGGTACTCAAGGTGTTGGAATATGTTCGCCCTAAGTACGCTTGCCGCCAGTGCGAGAAAA
CAGGCGACAGCAGCCATATCGTTCAGAAGCCATCCCCTCAGAGTCTCATCCCTAAAAGCTTCGCCACAGAAAGCTTGCTGGCCAACATCATTCTTGGCAA
ATACCAGTATGCGATGCCACTTTATCGCCAAGAATCGCTGTTTACCCAGTCGGGTATCGAGTTATCACGCACCACCATGGCAAGGTGGGTTATCCAAGTC
AGCGAGAAGTTCGCCCCGCTGTATGCCGCCTTGAAGAAGTACCTACTTCAGCAAGTGGTGGTTCAGGCGGATGAAACGCCGCTCAATGTGCTCAAGGAAG
AGAAGAAGTGTTATATGTGGCTCTACTGCTCGGGCGCTGACTCGCCCGAAGCCGCACTGCCTAATGTGAAAAATATCGCCTTGTACGACTATCAAAACAG
TCGCGCGAGGGCGTGCCCTATTGCCTTTTTAGGTGACTACAACGGTTATCTACAAACCGATGGCTACGCTGCTTATGATGGACTTCATCAAGTCACCAAT
GTGGGGTGCTTAGCGCATGCTCGTCGCAAGTTCATGGACGCGAAGAAGCTTCAAGGGAAAGGTAAGTCGGGCAAGGCCGATAAAGCGCTGGCTAAAATCC
AAAAACTCTACGGGATAGAATCGCGCTTAAAAGGTGCGCCTGCCGAAGAGCGAAAAGCAGAGCGTCAAGCACTTGCCAAGCCGATACTGGATGAGCTTTA
CCAATGGATGACAACCCAGAAGGTGATAGGCTCTAGCCCACTGGGCAAAGCGATAAAATACACGCTCGGGCAGTGGTCAAAGCTCACTCGTTATATCGAC
GATGGCCACTTATCTATCGATAATAATCGCGCTGAACGCGCAATTAAACCGCTGGTTATTGGCAGGAAGAACTGGCTATTCTCTAACACACCAAACGGTG
CTGATGCGAGCGCGATGCTTTACAGCATCATCGAGACCGCGAAAGCCAACGGTCTTATCCTCTATGACTACCTAGTCAAGTGCATGAAAGAGTTGGCGAA
AGCAGAGCCTGATATCGACGCACTCCTACCTTGGAACTTCAAACACTGACAAGTCGGCCCGTGGGTTCATGGGGCGGATAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
318 bp105 aa81398+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MAKRRTNLEWQSLFEQYESSSVTQRAFCEEHGLSLSTFFAKRRQLQTVNQSEFVGFVKAEIVEKTTKYQAQIATANMTLLINDVELSIPQGTPATYLAEL
IGALS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
354 bp117 aa395748+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MKGMLSAPDIYLYRESVDFRKSINGLAAIIENDTDLPLGSGALFLFTNKQRDKIKVLYWDKTGFALWYKRLEKAKYKWPSKEKNQVFTLTQFELDRLLSG
FTIIGHEPVKINDFTMS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1542 bp513 aa8082349+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEKTPNINPESQDIAELQAMVKALMLSQEQKESQWQQERQSLLEQLKLAFDRQFAKRSEALKPYDESQGDLFNEAECEAVKEEEVEVTTTITTKKRGKRK
PLPKTLPREVIELDLDDHEKQCSCCNHGLHKIGEDRSEKLEFTPAVLKVLEYVRPKYACRQCEKTGDSSHIVQKPSPQSLIPKSFATESLLANIILGKYQ
YAMPLYRQESLFTQSGIELSRTTMARWVIQVSEKFAPLYAALKKYLLQQVVVQADETPLNVLKEEKKCYMWLYCSGADSPEAALPNVKNIALYDYQNSRA
RACPIAFLGDYNGYLQTDGYAAYDGLHQVTNVGCLAHARRKFMDAKKLQGKGKSGKADKALAKIQKLYGIESRLKGAPAEERKAERQALAKPILDELYQW
MTTQKVIGSSPLGKAIKYTLGQWSKLTRYIDDGHLSIDNNRAERAIKPLVIGRKNWLFSNTPNGADASAMLYSIIETAKANGLILYDYLVKCMKELAKAE
PDIDALLPWNFKH

 

Blast result :
Comments
ISVa5 is 54%(orfA) aa similar to ISPa30, 74%(orfB) and 67%(orfC) to ISShes9.
References
1] K. Olav Holm and Erik Hjerde (2010) Direct submission