ISVa4

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Vibrio anguillarum
Vibrio anguillarum NB10
DNA section
IS Length : 1254 bp

Ends


IR Length : 29/36

IRL : TGTAATGGTACGGTTAACTTGGATGGTATTTAAGCCTTATAATTATCACT
IRR : TGTAATGATCCAGCTTTTTTGGAGAGTTTTTAAGCCACTTTTTGCATTTG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGTAATGGTACGGTTAACTTGGATGGTATTTAAGCCTTATAATTATCACTACTTATAAGGAATCTGATATGTCACCAAAACTACGAGTGCAAATCACCCC
TCAACAGAAGCTTGAATATGCCAAGATGATGGTTGAAGATGGTTACACCAATCAACAGATACAAGATATCAGTGGTGCTTGTTCATCAGCTGTAGTGCGC
TGGAAGCGCCAATATAAAGAAGAAATGTTAGGCATGACACCCACTAATAGAGTGGCATTAACACCAGAGCAGCAACGTATACAAGAGTTGGAAAAACAAC
TTGCTAGAGCCAAAAGAGACATCGATATATTAAAAAAAGCCAGCGCCTTGTTCATACAAAGTCAGTAAAGCCTGACTTTGTCAGAAAATTGAAGAAGGCG
CACCCAAAATTCACTATTTCTGAGCTTTGCCGAGTGCTTGGCGTCGCCAGCAGCTGTTTTTACTATACGAGAGTTGTATGCTCTGATGAGACCACCCTGC
ACGATAGAATTTACCGTATTGCAGAGGATTCTGATTTTACTTATGGCAAGCGCCGAATACACCGAGAACTGGTAAAAAGTGATGTAAAGATAGGTTTAGC
AAAAGTATCGAGACTGATGAAAGCCATTGAAATACCAGTTAAGTATCCGAAAAAAAGACATCGTTACAACGAGGTTGGTGAAGAAAGTATCTATGCACCT
AATATATTGAAAAGGCAGTTTACCCCCGAAACTCACAATACTCATTGGGTGACGGATGTGACCTATATTCGTTCCCATCAAGGTTGGAGTTACTTGGCAT
GCGTATTAGATCTAGCAACGAAAGAGGTGGTCGGATATTCGTTGTCACGCTCCCCTGATACCAATTTAACTTTGGCCGCATTAGACAATGCCATCCAAAA
ACGACGAGCCGACACAACTCAATTACTGTTGCACTCAGATCAAGGATGTCACTTTAGCTCACGAGCTTATAGGCATAGATTACAAGTGCTAAATATTACA
CAAAGCATGAGTAAACGAGGGTGTTGTTGGGATAATGCTGTGATGGAACGTTTTTTTCGTTCTTTAAAATCAGAGCGTTTAAACGAATTAAGCTTTATCA
ATCATCAGTCAATCGTAATGGAGGTAGAGAGCTACATTCGTTTTTACAACTATAAACGAAGTCATAGTGGGATTGATTATTTAACGCCAAATGAAAAATA
CCAACAAATGCAAAAAGTGGCTTAAAAACTCTCCAAAAAAGCTGGATCATTACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
300 bp99 aa69368+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSPKLRVQITPQQKLEYAKMMVEDGYTNQQIQDISGACSSAVVRWKRQYKEEMLGMTPTNRVALTPEQQRIQELEKQLARAKRDIDILKKASALFIQSQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
918 bp305 aa3081225+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SQKRHRYIKKSQRLVHTKSVKPDFVRKLKKAHPKFTISELCRVLGVASSCFYYTRVVCSDETTLHDRIYRIAEDSDFTYGKRRIHRELVKSDVKIGLAKV
SRLMKAIEIPVKYPKKRHRYNEVGEESIYAPNILKRQFTPETHNTHWVTDVTYIRSHQGWSYLACVLDLATKEVVGYSLSRSPDTNLTLAALDNAIQKRR
ADTTQLLLHSDQGCHFSSRAYRHRLQVLNITQSMSKRGCCWDNAVMERFFRSLKSERLNELSFINHQSIVMEVESYIRFYNYKRSHSGIDYLTPNEKYQQ
MQKVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1157 bp385 aa691225+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSPKLRVQITPQQKLEYAKMMVEDGYTNQQIQDISGACSSAVVRWKRQYKEEMLGMTPTNRVALTPEQQRIQELEKQLARAKRDIDILKKSQRLVHTKSV
KPDFVRKLKKAHPKFTISELCRVLGVASSCFYYTRVVCSDETTLHDRIYRIAEDSDFTYGKRRIHRELVKSDVKIGLAKVSRLMKAIEIPVKYPKKRHRY
NEVGEESIYAPNILKRQFTPETHNTHWVTDVTYIRSHQGWSYLACVLDLATKEVVGYSLSRSPDTNLTLAALDNAIQKRRADTTQLLLHSDQGCHFSSRA
YRHRLQVLNITQSMSKRGCCWDNAVMERFFRSLKSERLNELSFINHQSIVMEVESYIRFYNYKRSHSGIDYLTPNEKYQQMQKVA

 

Blast result :
Comments
ISVa4 is 73% aa similar to ISSde8.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] K. Olav Holm (2010) Direct submission