ISGeob7

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013757 ND Geodermatophilus obscurus
Geodermatophilus obscurus DSM 43160
DNA section
IS Length : 883 bp

Ends


IR Length : 16/20

IRL : CAAGTCGTTTCACTATGACCTCGGCGTGGCTGCCGTAGGACGTGGCCAGT
IRR : CAACTCGTTTCAGAACGACCGAAGCCGGCGGTAGCAGATCAAGGCGCAGG

Insertion site


Left flankDirect repeatRight flankDR Length
CACGAGGTCGTAAGCGTGACCTT3
CGCGCCCCACTAATGCGCCACCT3

DNA sequence

CAAGTCGTTTCACTATGACCTCGGCGTGGCTGCCGTAGGACGTGGCCAGTCTGAGCAGGCTGGCCTGGGTGATCGAGGAGCTTGTCCCCGACGGGCTGTG
GCAGCGGATCGCTTCGCTGCTGCCACCACCGAAGCCCCGCCGCCACCGCTATCCGGGGCGACGGCCGATCGATGACCGCGCCGCACTCGCCGGGATCATG
TTCGTGCTCAAGACCGGCATCACCTGGAACCAACTGCCCACGTCGCTGGTCGGCTGCTCCGGGGTGACCTGCTGGCGGCGGCTGCGCGACTGGACCGAAG
CCGGCGTGTGGCCGGCGCTGCACGAGCTGCTGCTCGCCGAGCTGCGCGCGGCCGGCGAGTTGGACCTGGACCGCTGCGCGGTCGACGGCTCCCACATCCG
GGCACTCAAAGGGGGGACCAGGTCGGCCCCTCACCGGTCGACCGCGGACGCCCCGGCTCCAAGCACCACCTGATCTGCGACGCCGACGGCATCCCGCTGG
CCGTCACGCTGACCGGCGGCAACCGCAACGACGTCACCCAGCTCATCCCGCTGGTCGACGCGGTGCCGCCGATTAGGGGTCGGCGCGGTCGGCCGCGTCG
TCGACCGCGCGAGCTGTTCGCCGACCGCGGCTACGACCACGACATCTACCGTCGCCGGCTGCGCGCTCGCGGCATCACTCCGCGGATCGCCCGCCGCGGC
GTCGCCCACGGCTCGGGTCTGGGCAAGCAGCGCTGGGTGGTCGAACGAAGCTTCGCCTGGCTGCACGCGTTCAAGCGGCTACGCACCCGGTATGAACGCC
GCGCCGACATCCACCTCGGGCTGATGCAACTGGCCTGCGCCTTGATCTGCTACCGCCGGCTTCGGTCGTTCTGAAACGAGTTG
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
405 bp134 aa69473+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MIEELVPDGLWQRIASLLPPPKPRRHRYPGRRPIDDRAALAGIMFVLKTGITWNQLPTSLVGCSGVTCWRRLRDWTEAGVWPALHELLLAELRAAGELDL
DRCAVDGSHIRALKGGTRSAPHRSTADAPAPSTT

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
516 bp171 aa359874+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VGPGPLRGRRLPHPGTQRGDQVGPSPVDRGRPGSKHHLICDADGIPLAVTLTGGNRNDVTQLIPLVDAVPPIRGRRGRPRRRPRELFADRGYDHDIYRRR
LRARGITPRIARRGVAHGSGLGKQRWVVERSFAWLHAFKRLRTRYERRADIHLGLMQLACALICYRRLRSF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
806 bp268 aa69874+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIEELVPDGLWQRIASLLPPPKPRRHRYPGRRPIDDRAALAGIMFVLKTGITWNQLPTSLVGCSGVTCWRRLRDWTEAGVWPALHELLLAELRAAGELDL
DRCAVDGSHIRALKRGDQVGPSPVDRGRPGSKHHLICDADGIPLAVTLTGGNRNDVTQLIPLVDAVPPIRGRRGRPRRRPRELFADRGYDHDIYRRRLRA
RGITPRIARRGVAHGSGLGKQRWVVERSFAWLHAFKRLRTRYERRADIHLGLMQLACALICYRRLRSF

 

Blast result :
Comments
ISGeob7 is 72% aa similar to ISBugl2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2010)
2) Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Munk,A.C., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,M., Schneider,S., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.