ISfinder Mirror Site

ISPaen3

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_GG695982	ND	Paenibacillus sp.	Paenibacillus sp. oral taxon 786 D14

DNA section

IS Length : 914 bp

Ends

IR Length : 15/17

IRL : GAGCCTATCCGAAAACCATTATAAGCAAGTAGGAAAACGGTGGAACATGT
IRR : GTACCTATCCGAAAACCTCTGCGCAGCGGAAACAAATCCAGGCGCAGGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TAGCATGGAA	TAA	ATCAAATCAT	3

DNA sequence

GAGCCTATCCGAAAACCATTATAAGCAAGTAGGAAAACGGTGGAACATGTCGAATTCCTTCAAGAGAAGCACTTGGAGGAGATCGTCATGGCAAATGCAC
CGAAGCAACTCTTTTCGATTCCCGATGAACTGTGGGAGAAAATCCAACCTTTGCTACCGGAGGAACCGCCAAAACCGAAGGGCGGTCGTCCGCGCATGGA
TGATCGTCAGGCGATGACGGCGATTTTTTACGTGCTCCGCACGGGCATCCAGTGGAAAGCGTTGCCCCGTCAAATGGGGGCCGCCAGTATGGTGCATGAC
CGCTTTCAATATTGGCAGGAACAAGGCGTATTCAAACGGATGTGGGAGGCCGGGTTGCAAGCGTACGACGAGGCCAAAGGAATCGAATGGACCTGGCAAG
CGGTGGACGGAGCCATGACCAAGGCTCCGCTTGGGGGAAAAAAAGACGGGTCCTAACCCGACGGATCGCGGCAAGCGTGGAACCAAGCGGAGTTTGTGGA
CGGATGGACGCGGCATGCCCTTGGGCGTCGTCGTCGATGGGGCGAATCGTCACGATATGAAACTGCTGGGGCCCACGCTGTCCGAGCCGGCTGCTCCGCG
ACCGGAACCGGACGCGAAGAACCCGCAGCATTTGTGCTTGGATAAGGGATACGACTATCCGATCATCCGGGAACAAGTGGCCGAGTTGGGATATGTCCTT
CATTTGCGTTCGCGCGGCGAAGAGAAAGAAGAAAAAGCGGCGATTCCCGGATACCGGGCCAGACGATGGGTGGTCGAACGGACCCACTCCTGGATGAATC
GCTTTCGCCGCTTGCTCATTCGTTGGGAAAAGAAAGTGGAGAACTACGAGGCCATGCTCCATTTTGCCTGCGCCTGGATTTGTTTCCGCTGCGCAGAGGT
TTTCGGATAGGTAC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
369 bp	122 aa	88	456	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MANAPKQLFSIPDELWEKIQPLLPEEPPKPKGGRPRMDDRQAMTAIFYVLRTGIQWKALPRQMGAASMVHDRFQYWQEQGVFKRMWEAGLQAYDEAKGIE
WTWQAVDGAMTKAPLGGKKDGS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
492 bp	163 aa	419	910	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PRLRLGEKKTGPNPTDRGKRGTKRSLWTDGRGMPLGVVVDGANRHDMKLLGPTLSEPAAPRPEPDAKNPQHLCLDKGYDYPIIREQVAELGYVLHLRSRG
EEKEEKAAIPGYRARRWVVERTHSWMNRFRRLLIRWEKKVENYEAMLHFACAWICFRCAEVFG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
823 bp	273 aa	88	910	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MANAPKQLFSIPDELWEKIQPLLPEEPPKPKGGRPRMDDRQAMTAIFYVLRTGIQWKALPRQMGAASMVHDRFQYWQEQGVFKRMWEAGLQAYDEAKGIE
WTWQAVDGAMTKAPLGGKKTGPNPTDRGKRGTKRSLWTDGRGMPLGVVVDGANRHDMKLLGPTLSEPAAPRPEPDAKNPQHLCLDKGYDYPIIREQVAEL
GYVLHLRSRGEEKEEKAAIPGYRARRWVVERTHSWMNRFRRLLIRWEKKVENYEAMLHFACAWICFRCAEVFG

Blast result :

Comments

ISPaen3 is 75% aa similar to ISMba5. The third ORF is the putative transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2010)
2] Ward,D., Young,S.K., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Hepburn,T., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Walk,T., White,J., Yandava,C., Allen-Vercoe,E., Strauss,J., Sibley,C., White,A., Lander,E., Nusbaum,C., Galagan,J. and Birren,B. (2009) Direct submission GenBank.