ISPist1
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013720 | ND | Pirellula staleyi | Pirellula staleyi DSM 6068 |
DNA section
IS Length : 920 bp
Ends
IR Length : 18/20
IRL : GAGCCTATCCGATAACCCGAAATTGGTATTGAAAAAGAGGTGAGTCCTTT
IRR : GAGCCTATCCGAGAACCTGAGCTTGTTTGAGGGTATAGAAGGCGCAGGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTTCCGTACTTA | TAAAGGTTGTTGA | 0 |
DNA sequence
GAGCCTATCCGATAACCCGAAATTGGTATTGAAAAAGAGGTGAGTCCTTTTGTTAAAGGAGAGTTACCACACTCGCACCCTTACAAAAGGACCCACCATG
GACGGTGCTGTTAGCTTTGATGCCTATCGAATGCCCGACGAATTGTGGCAGCAAATGCAATTCCTGTTGCCTCCCTACATTCCCTCCCCCTTGGGCGGAC
GGCCCAGAGCCGTACTACGCGACGTAGCAGATGCCATCTTTTACCGAATTCGCACAGGCTGTCAATGGAAAGCAATACCACGCTGCCTCGCTGCGGGAAG
CACCGCCCACAGCTACTTTCAGCAATGGGTAGAGCAAGGCGTATTCGACGATCTGTGGCAGCTAGCGCTCGAACTGTTTGACGACCTCGTGGGACTCGAC
TGGCGCTGGCAAGCGGTCGATGGAGCGATGACCAAAGCGCCCCTAGGCGGCGAAGCGACCGGCAAAAACCCCACCGATCGGGCCAAGCTGGGAACCAAAC
GCTCGCTCATGACCGATGCTCGTGGCATCCCCGTTGGGCTCGCCGTCTCTGGAGCCAATACCCACGATTCACGTCTTCTTCAAGCAACCTTGGAGAACCG
TCTTTTAAGATTCCCAAGAGATCCTAACTCCCGTTGTGAACGTCTTCTATTAGATAAAGCTTATGACTCCCAAGCTATTCGCACGCTGGTGGAAGAGCAG
CTGTCGTTTCGAGCTCACATTCGCTCACGCCATCAAGAGATTCGGGAAAAGGAGCGTGCTCGACCCAGGCGGTGGGTCGTCGAGCGTACTCACAGCTGGC
TCAACCGCTTTCGCGGCGTACTGCTCCGATGGGAGAAGAAAGTCGACAATCACATCGCGATCCTGCAACTGGCCTGCGCCTTCTATACCCTCAAACAAGC
TCAGGTTCTCGGATAGGCTC
GACGGTGCTGTTAGCTTTGATGCCTATCGAATGCCCGACGAATTGTGGCAGCAAATGCAATTCCTGTTGCCTCCCTACATTCCCTCCCCCTTGGGCGGAC
GGCCCAGAGCCGTACTACGCGACGTAGCAGATGCCATCTTTTACCGAATTCGCACAGGCTGTCAATGGAAAGCAATACCACGCTGCCTCGCTGCGGGAAG
CACCGCCCACAGCTACTTTCAGCAATGGGTAGAGCAAGGCGTATTCGACGATCTGTGGCAGCTAGCGCTCGAACTGTTTGACGACCTCGTGGGACTCGAC
TGGCGCTGGCAAGCGGTCGATGGAGCGATGACCAAAGCGCCCCTAGGCGGCGAAGCGACCGGCAAAAACCCCACCGATCGGGCCAAGCTGGGAACCAAAC
GCTCGCTCATGACCGATGCTCGTGGCATCCCCGTTGGGCTCGCCGTCTCTGGAGCCAATACCCACGATTCACGTCTTCTTCAAGCAACCTTGGAGAACCG
TCTTTTAAGATTCCCAAGAGATCCTAACTCCCGTTGTGAACGTCTTCTATTAGATAAAGCTTATGACTCCCAAGCTATTCGCACGCTGGTGGAAGAGCAG
CTGTCGTTTCGAGCTCACATTCGCTCACGCCATCAAGAGATTCGGGAAAAGGAGCGTGCTCGACCCAGGCGGTGGGTCGTCGAGCGTACTCACAGCTGGC
TCAACCGCTTTCGCGGCGTACTGCTCCGATGGGAGAAGAAAGTCGACAATCACATCGCGATCCTGCAACTGGCCTGCGCCTTCTATACCCTCAAACAAGC
TCAGGTTCTCGGATAGGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 819 bp | 272 aa | 98 | 916 | + | No |
Chemistry : DDE
ORF sequence :
MDGAVSFDAYRMPDELWQQMQFLLPPYIPSPLGGRPRAVLRDVADAIFYRIRTGCQWKAIPRCLAAGSTAHSYFQQWVEQGVFDDLWQLALELFDDLVGL
DWRWQAVDGAMTKAPLGGEATGKNPTDRAKLGTKRSLMTDARGIPVGLAVSGANTHDSRLLQATLENRLLRFPRDPNSRCERLLLDKAYDSQAIRTLVEE
QLSFRAHIRSRHQEIREKERARPRRWVVERTHSWLNRFRGVLLRWEKKVDNHIAILQLACAFYTLKQAQVLG
DWRWQAVDGAMTKAPLGGEATGKNPTDRAKLGTKRSLMTDARGIPVGLAVSGANTHDSRLLQATLENRLLRFPRDPNSRCERLLLDKAYDSQAIRTLVEE
QLSFRAHIRSRHQEIREKERARPRRWVVERTHSWLNRFRGVLLRWEKKVDNHIAILQLACAFYTLKQAQVLG
Blast result :
Comments
ISPist1 is 67% aa similar to ISMac11.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Last,F.I., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Fahnrich,R., Schneider,S., Tindall,B.J., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Last,F.I., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Fahnrich,R., Schneider,S., Tindall,B.J., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank.