ISMepa1
- Family IS5
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011832 | ND | Candidatus Methanosphaerula | Candidatus Methanosphaerula palustris E1-9c |
DNA section
IS Length : 1009 bp
Ends
IR Length : 13
IRL : GGCTCTGTTGAAACCCTCTGGTACTATGATCCCACGATAAGACAAATATC
IRR : GGCTCTGTTGAAATGCTTCAACTATGATAACAATCACGACTGATTGAACC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAGAACCTGT | CTTCTTTG | ATAAGGATCAGT | 8 |
| GGAATAGATA | TCAATAAG | CTGTTAAAAGCC | 8 |
| TAATAAAATG | GAAAAATT | TATAGAAAAATC | 8 |
| TCTGATCCTT | CGGGAACC | GTAAATAGAAAC | 8 |
| CAATTGATT | GAAATTCA | GTCATGGTGAAC | 8 |
DNA sequence
GGCTCTGTTGAAACCCTCTGGTACTATGATCCCACGATAAGACAAATATCTAATAAGAATCGTTGCTCCTTGAGTCAATGACAAATTCAACAACAAGGAG
CAGAAATAATGTCAACGAACCGGTATATCAAACTTGTCAGATACATCTGTTCAGTACTTCGATCCTCCCATTTCCCTCTTTATTCCTGTAAATATTCTCG
GAGAACGTATACTCAGCATCAGCTCATGGCGATCCTTCTCTTTCGTGAAGCCCTTGGTACTGACTATCGTGATGTAGTCGAACTCATCAATCTGATGGGC
AGGATCAAAGACATCCTCAATTTTGACCAGGTTCCCCACTACTCGACCATCCATAAGTTCATGGCTCGAACTCCTTCTGCAGTACTCTCGATATTCCTGA
ATAAAACCCTCAAACTGTTTTATTCCTGGGGTGAAATTGTTCCAACAACTGCCATCGATTCTACGGGATTCACAAGTCCGTACGCCAGTCATTATTACTC
CTGGCGTACAGGAAAGACCCGAAAAAACTTCCTGAAAACGTCGATTGCAGTAGATACATGTAAGCAGGTGATCCTCTTTTCCAAGATCTCGCTAAAACCA
GTTCACGACACTAAACATGCTGAACCACTCCTCAGGCAGTGCCAGAGGACCAGAAAAACCGGGTGTTATGTGATGGATAAAGGGTACGATTCCGAGAAAC
TTCATCGACAGATCAGGGAAGAAATGGGTGCTGATTCGGTGATACCTGTCAGAACCTGGAAAGGAAAGATCTATTCGGGGAAATATAGACAAGAGATGTA
TAACAACTTCGATTCGAAACGCTATTACGAGAGAAACAAAGTTGAAACCGCATTTTCAGTCATTAAAAGAAGATTCGGAGAGGACCTCAAGGCACGAAAA
TATTGGTACCAGGTCAAGGAGATCAAGGTCAAAATGATCCTCCATAACCTCACGAAGGTGGTTCAATCAGTCGTGATTGTTATCATAGTTGAAGCATTTC
AACAGAGCC
CAGAAATAATGTCAACGAACCGGTATATCAAACTTGTCAGATACATCTGTTCAGTACTTCGATCCTCCCATTTCCCTCTTTATTCCTGTAAATATTCTCG
GAGAACGTATACTCAGCATCAGCTCATGGCGATCCTTCTCTTTCGTGAAGCCCTTGGTACTGACTATCGTGATGTAGTCGAACTCATCAATCTGATGGGC
AGGATCAAAGACATCCTCAATTTTGACCAGGTTCCCCACTACTCGACCATCCATAAGTTCATGGCTCGAACTCCTTCTGCAGTACTCTCGATATTCCTGA
ATAAAACCCTCAAACTGTTTTATTCCTGGGGTGAAATTGTTCCAACAACTGCCATCGATTCTACGGGATTCACAAGTCCGTACGCCAGTCATTATTACTC
CTGGCGTACAGGAAAGACCCGAAAAAACTTCCTGAAAACGTCGATTGCAGTAGATACATGTAAGCAGGTGATCCTCTTTTCCAAGATCTCGCTAAAACCA
GTTCACGACACTAAACATGCTGAACCACTCCTCAGGCAGTGCCAGAGGACCAGAAAAACCGGGTGTTATGTGATGGATAAAGGGTACGATTCCGAGAAAC
TTCATCGACAGATCAGGGAAGAAATGGGTGCTGATTCGGTGATACCTGTCAGAACCTGGAAAGGAAAGATCTATTCGGGGAAATATAGACAAGAGATGTA
TAACAACTTCGATTCGAAACGCTATTACGAGAGAAACAAAGTTGAAACCGCATTTTCAGTCATTAAAAGAAGATTCGGAGAGGACCTCAAGGCACGAAAA
TATTGGTACCAGGTCAAGGAGATCAAGGTCAAAATGATCCTCCATAACCTCACGAAGGTGGTTCAATCAGTCGTGATTGTTATCATAGTTGAAGCATTTC
AACAGAGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 901 bp | 300 aa | 109 | 1009 | + | No |
Chemistry : DDE
ORF sequence :
MSTNRYIKLVRYICSVLRSSHFPLYSCKYSRRTYTQHQLMAILLFREALGTDYRDVVELINLMGRIKDILNFDQVPHYSTIHKFMARTPSAVLSIFLNKT
LKLFYSWGEIVPTTAIDSTGFTSPYASHYYSWRTGKTRKNFLKTSIAVDTCKQVILFSKISLKPVHDTKHAEPLLRQCQRTRKTGCYVMDKGYDSEKLHR
QIREEMGADSVIPVRTWKGKIYSGKYRQEMYNNFDSKRYYERNKVETAFSVIKRRFGEDLKARKYWYQVKEIKVKMILHNLTKVVQSVVIVIIVEAFQQS
LKLFYSWGEIVPTTAIDSTGFTSPYASHYYSWRTGKTRKNFLKTSIAVDTCKQVILFSKISLKPVHDTKHAEPLLRQCQRTRKTGCYVMDKGYDSEKLHR
QIREEMGADSVIPVRTWKGKIYSGKYRQEMYNNFDSKRYYERNKVETAFSVIKRRFGEDLKARKYWYQVKEIKVKMILHNLTKVVQSVVIVIIVEAFQQS
Blast result :
Comments
ISMepa1 is 72% aa similar to ISMbu10.
There is no STOP codon in the insertion sequence, the transposase use the first after the end of the IS.
There is no STOP codon in the insertion sequence, the transposase use the first after the end of the IS.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Lu,M., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G.O. and Zinder,S. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Lu,M., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G.O. and Zinder,S. (2009) Direct submission GenBank.