ISPna5
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008757 | ND | Polaromonas naphthalenivorans | Polaromonas naphthalenivorans CJ2 Polaromonas naphthalenivorans CJ2 plasmid pPNAP01 |
DNA section
IS Length : 1088 bp
Ends
IR Length : 18
IRL : GGCGTTGTTGCATAAATCGCCCGCGCCAATGAACGGGCTGCCTTACTGAT
IRR : GGCGTTGTTGCATAAATCCTCTGTGAGGATGAGGCTTGCCCCTTACCCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGCACGCCGCGCGCTTGCGC | ACGTGCCGCACCGCGAGACTT | 0 | |
| GCGTGGCCAG | GCACAGGT | GGCCGGTTTC | 8 |
DNA sequence
GGCGTTGTTGCATAAATCGCCCGCGCCAATGAACGGGCTGCCTTACTGATAGGCTACCGACCTTTGTTCCGAACATGCGAAAAGACAAGCACCTGCCCAG
CCAGCCCAAGGGGGTCTACCGGGTCAAGAACTGGCCCGAGTACAACGCGGGCCTGATTGAGCGGGGCAATGTGAGCATCTGGATGGACGAAGAGATGTTC
GCACCAATGGCTGGGGCGTCAGTTCAGCGCGGGCGCCCGCAGGCTTACTGCGATGCAGTGATTCAGATGCTGCTGGCCCTCAAGTCGGTGTACCGCCTGC
CGCTGCGGGCATTGCAGGGGTTTGCCATGAGCCTGCGCCGCCTGGCGCTGCCGGCCTTGCCAGTGCCCAACTACAGCACGCTGAGCCGAAGGGCCAAGGC
GCTGCGGGTAACCTTGCCGGTGCTGCGCAACGCAGGCGAGGCTGTGCATCTGCTGGTGGACAGCACGGGGTTGAAGCTGTTTGGCGAGGGCGAATGGAAG
GTGCGTAAACACGGCTACGCCAAGCGCCGCAGCTGGCGCAAAGTGCATCTGGGCATGGATGACGAGACGGGGCAGGTGTGCGCCGTGTTGATGACGCACA
AGGATGTGATTGATGCCAGTGTGCTGCCGGAGTTACTGGCGCAAATCCCCCAAGAAACGAAGATTGAAGTGGTTATCGGCGATGGTGCCTACGATACAAA
GGCCGCCCGTGCGGCCATTGCCGGGTGCGGCGCGTTGGCGGTCATTCCCCCCGTGGAGGGCGCCGTGCACTGGCCTGCCAGCCAGGCTGGGGCGCCAGAG
CGCAACGAGGCCATTGACCATATTGCCCAAAGCAGCAAGCAGGACTGGAAAGACAAAAGTGGCTACCATCGCCGTTCCCTGGTGGAGAACCTGATGTACC
GCTTCAAGGTGCTGACGGGCGACAGGCTGTGGGCGCGCGATGTGGATGTGCAAGACGCTGAAGTCGCTGTTCGGGTGGGCATCATCAATCGCATGCTGGT
GCTTGCACGCCCGAAATCCGTCCGGATTGCCTAAAACGCGGGGTAAGGGGCAAGCCTCATCCTCACAGAGGATTTATGCAACAACGCC
CCAGCCCAAGGGGGTCTACCGGGTCAAGAACTGGCCCGAGTACAACGCGGGCCTGATTGAGCGGGGCAATGTGAGCATCTGGATGGACGAAGAGATGTTC
GCACCAATGGCTGGGGCGTCAGTTCAGCGCGGGCGCCCGCAGGCTTACTGCGATGCAGTGATTCAGATGCTGCTGGCCCTCAAGTCGGTGTACCGCCTGC
CGCTGCGGGCATTGCAGGGGTTTGCCATGAGCCTGCGCCGCCTGGCGCTGCCGGCCTTGCCAGTGCCCAACTACAGCACGCTGAGCCGAAGGGCCAAGGC
GCTGCGGGTAACCTTGCCGGTGCTGCGCAACGCAGGCGAGGCTGTGCATCTGCTGGTGGACAGCACGGGGTTGAAGCTGTTTGGCGAGGGCGAATGGAAG
GTGCGTAAACACGGCTACGCCAAGCGCCGCAGCTGGCGCAAAGTGCATCTGGGCATGGATGACGAGACGGGGCAGGTGTGCGCCGTGTTGATGACGCACA
AGGATGTGATTGATGCCAGTGTGCTGCCGGAGTTACTGGCGCAAATCCCCCAAGAAACGAAGATTGAAGTGGTTATCGGCGATGGTGCCTACGATACAAA
GGCCGCCCGTGCGGCCATTGCCGGGTGCGGCGCGTTGGCGGTCATTCCCCCCGTGGAGGGCGCCGTGCACTGGCCTGCCAGCCAGGCTGGGGCGCCAGAG
CGCAACGAGGCCATTGACCATATTGCCCAAAGCAGCAAGCAGGACTGGAAAGACAAAAGTGGCTACCATCGCCGTTCCCTGGTGGAGAACCTGATGTACC
GCTTCAAGGTGCTGACGGGCGACAGGCTGTGGGCGCGCGATGTGGATGTGCAAGACGCTGAAGTCGCTGTTCGGGTGGGCATCATCAATCGCATGCTGGT
GCTTGCACGCCCGAAATCCGTCCGGATTGCCTAAAACGCGGGGTAAGGGGCAAGCCTCATCCTCACAGAGGATTTATGCAACAACGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 960 bp | 319 aa | 75 | 1034 | + | No |
Chemistry : DDE
ORF sequence :
MRKDKHLPSQPKGVYRVKNWPEYNAGLIERGNVSIWMDEEMFAPMAGASVQRGRPQAYCDAVIQMLLALKSVYRLPLRALQGFAMSLRRLALPALPVPNY
STLSRRAKALRVTLPVLRNAGEAVHLLVDSTGLKLFGEGEWKVRKHGYAKRRSWRKVHLGMDDETGQVCAVLMTHKDVIDASVLPELLAQIPQETKIEVV
IGDGAYDTKAARAAIAGCGALAVIPPVEGAVHWPASQAGAPERNEAIDHIAQSSKQDWKDKSGYHRRSLVENLMYRFKVLTGDRLWARDVDVQDAEVAVR
VGIINRMLVLARPKSVRIA
STLSRRAKALRVTLPVLRNAGEAVHLLVDSTGLKLFGEGEWKVRKHGYAKRRSWRKVHLGMDDETGQVCAVLMTHKDVIDASVLPELLAQIPQETKIEVV
IGDGAYDTKAARAAIAGCGALAVIPPVEGAVHWPASQAGAPERNEAIDHIAQSSKQDWKDKSGYHRRSLVENLMYRFKVLTGDRLWARDVDVQDAEVAVR
VGIINRMLVLARPKSVRIA
Blast result :
Comments
ISPna5 is 91% aa similar to ISPna4.
References
1] ISfinder annotation (2010)
2] Yagi,J.M., Sims,D., Brettin,T., Bruce,D. and Madsen,E.L. (2009) Environ. Microbiol. 11 (9), 2253-2270
3] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D.R., Brettin,T., Bruce,D., Han,C., Tapia,R., Brainard,J., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Madsen,E.L. and Richardson,P. (2007) Direct submission GenBank.
2] Yagi,J.M., Sims,D., Brettin,T., Bruce,D. and Madsen,E.L. (2009) Environ. Microbiol. 11 (9), 2253-2270
3] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D.R., Brettin,T., Bruce,D., Han,C., Tapia,R., Brainard,J., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Madsen,E.L. and Richardson,P. (2007) Direct submission GenBank.