ISNph15

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007426 ND Natronomonas pharaonis
Natronomonas pharaonis DSM 2160
DNA section
IS Length : 1476 bp

Ends


Left end : AGGCGGTACAAGGCGACTGCCCCGGGGCTTGACCCCGGGGTTGAAGCCGACAGCTTTATACTGATTCAAACAGTATAATACGGTATGGGTCAGATGGAAT II struct. : Yes
Right end : ACTGCGCTCCCTACGCTCACGCCTCACCGGGAGGCTGTGAGTGCAAAGCGCGTCGTTGAAGCAGGAAGCCCCGGGGCTTGACCCCCCGGGGTGAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
AAGATATCCTGTACGACCATGAATTCGAtcac

DNA sequence

AGGCGGTACAAGGCGACTGCCCCGGGGCTTGACCCCGGGGTTGAAGCCGACAGCTTTATACTGATTCAAACAGTATAATACGGTATGGGTCAGATGGAAT
GTTACCCGAAGATGCGACAGCGCGGTGTCGTTACAATCCCAGAAGAAGTCCGAGACGGGCTTAATCTGGAAGAAGGCGACCAGTTGAAACTCACTGTGGA
AAAACTTGACTAACACCGATGAAACACACGCTTCGCTTCCGCGCGTACCTGCCCGACGACGTGGCAAGCGAAGCGTGGCGGCACATCGACATTCTCCGGC
AGATTCGTAATCACGCTGTCCGAGACTACTACAACGCAGACTACAACGACAGGCCATCTGACTACGACCAACACAGCAAACTCAAAGACTGGACAGACCA
GTGGCCGACCTTTGCCGACCCGTCACAACACGCCGCACAACAGGCTATCAGCCAAATTCACAGCGACATAGAGACGCTGCAAGAACGCCGAAACGAAGGC
TACGATGTTGGGCGGTTGCAGTGGCAAGGAGCAGGCGAATTTCGGTCAGTGTCGTACAATCAGGCAAGTCGCTTCAACGTGGATTACAACACGGGCGACG
ACCGATTCGTGCGACTCCGACTCGAAAAGATTGGCTGGTTCAAAATCCGAGCAGACCGCGACGTGCCACCAGAAGAGGACATTGACGAGGTTATCCTCAA
AAAAGAGACGACTGGCGAATGGTACGTCTCACTCGTCACCACTGTCGAAGACACACCCGAGAAACCGCCACTCAGTGAGATTGAAGCCGAGGACTGCGTG
GGCGTTGACCTTGGCATCACCAGCTACATCCACACTTCGGAGAACCTGTCCGTTGATACACTCGACCTGTCCGATGAGTACGACCGCTACGCACGCGAAC
AGCGGAAACTTGACCGGAAAGAACACGGCTCCGCCAACTGGGAGAAACAGCGTCAGAAGGTTGCCCAAGCGAAACGAAAAATCAAACGGAAGGTGCGAGA
CTACCAGCACAAGTTGACGACGTGGCTCGTCACAGAGCACGATGTTGTAGCTGTCGAAGACTTGGATGTGAAACCGATGCTGGAAGCCAGTCAGAACGCC
AAGAACAAGCAGGATGCCGCGTGGTCGCAGTTTATCGAACTGCTGGAGTACAAGGCTGACCTACACGGCACACACGTCGAAACGGTGAAGCCGGGAGGAA
CAACCAAAGAGTGCGCTGTGTGTGGTGTCGAAACAGCCAAGCCAATCTGGGTACGCGAACACTCTTGTCCAGCGTGCGGCCATACGGAAGCCCGCGACCT
CAACGCGGCGAAGAATATTCTCTATCGCGGACTCAAGCAGTTAGGGGCGGGACGCTCCGAATCAACGCCTGTGCAGACTGCGCTCCCTACGCTCACGCCT
CACCGGGAGGCTGTGAGTGCAAAGCGCGTCGTTGAAGCAGGAAGCCCCGGGGCTTGACCCCCCGGGGTGAGTTCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1239 bp412 aa2191457+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKHTLRFRAYLPDDVASEAWRHIDILRQIRNHAVRDYYNADYNDRPSDYDQHSKLKDWTDQWPTFADPSQHAAQQAISQIHSDIETLQERRNEGYDVGRL
QWQGAGEFRSVSYNQASRFNVDYNTGDDRFVRLRLEKIGWFKIRADRDVPPEEDIDEVILKKETTGEWYVSLVTTVEDTPEKPPLSEIEAEDCVGVDLGI
TSYIHTSENLSVDTLDLSDEYDRYAREQRKLDRKEHGSANWEKQRQKVAQAKRKIKRKVRDYQHKLTTWLVTEHDVVAVEDLDVKPMLEASQNAKNKQDA
AWSQFIELLEYKADLHGTHVETVKPGGTTKECAVCGVETAKPIWVREHSCPACGHTEARDLNAAKNILYRGLKQLGAGRSESTPVQTALPTLTPHREAVS
AKRVVEAGSPGA

 

Blast result :
Comments
ISNph15 is 69% (ORFB) aa similar to ISNph10. There is an empty site in Haloterrigena turkmenica DSM 5511 genome, this IS disrupt the gene of the formate dehydrogenase, alpha subunit.
References
1] Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J., Oesterhelt D. (2005) Genome Res. 15: 1336-1343