ISButh7
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007650 | ND | Burkholderia thailandensis | Burkholderia thailandensis E264 chromosome II |
DNA section
IS Length : 1128 bp
Ends
IR Length : 22/23
IRL : GGCGTTGTTGCATAAATCAGGCCGCGTCGCGTGACGTTTACGCGCAACAG
IRR : GGACGTTGTTGCATAAATCAGGCGTGAGGACGAAGTGGTGCCTTTGTTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAACCTGCAC | ATTCGAGC | GCCGAACGTC | 8 |
DNA sequence
GGCGTTGTTGCATAAATCAGGCCGCGTCGCGTGACGTTTACGCGCAACAGGCGGAGCAATTCCTCTGTTAACACTTCGGGTTCCTGCGTAGATTCTCGAT
CTTTGGTCGCGAGAACATGCGCAAGGATAAGAACGGGACGCAGGAGCCGAAGGCGAAATACCGTGTCAGAAACTGGGCGGCATACAACGCCGGCCTGATC
AATCGAGGCAACATCACGATGTGGATTGGTGACGACGCTCTTGCGAACATGCCCGACACCGAGCCCACACGTGGCCGTTCGCGGCTGTACAGCGATGCAC
TGATTCAGGCATTGCTCGGCTTGAAGACGGTGTTCCATCTGCCGTTGCGTGCCTTGCAAGGCTTCGCGCAAAGCCTGCGTGACCTGGCTTTCGCTGAATT
GCCCGTACCGAACTATACGACCGTGTGCCGTCGCGCACAGGCGCTGCAGGTGAAACTGCCGGTCATTCACAGCGACGAACCGCTGCACTTGGTCGTCGAC
AGCACGGGCGCAAAGGTCTGCGGCGAAGGCGAATGGAAGGTTCGCCAGCATGGCTACTCGAAGCGTCGCACGTGGCGCAAGGTCCATCTCGCACTCGACG
CGAACACGGGCCAGTTGCGAGCGGCGCTCATGACGCACCAGGATGTCGCAGACGGTGAAGTGCTGCCCGAATTGCTTGAACAAATCTCGGCCGACGAGTC
CATCGATACCATCGGCGGCGACGGCGCATACGATACGCAGGCTTGTTACGCCTCGATCACCGCGCGCGGTGCGTTACCCTCGATTCCGCCGCGCGAAGGC
GCGGTTCACTGGCCGACGACCACGCCCGGCGCGTCTCGGCGCAACGCCGCGCTCGACGCCATTGCACAAGGCAGTCGACGCGAATGGAAGCAGCAAAGCG
GTTACCACCGTCGTTCGCTGGCCGAGAACGCGATGTACCGGCTCAAGACGCTCACGGGTCCGTGCCTGTGGGCACGTCGTACCGATACCCAGGCGACCGA
AGTTGCCGTTCGAGTGGGCGTGCTTAACCGCATGGCCGAGCTCTCCCGCCCTCAGTCCGTCCGTATTGCCCGAGATCATGAACAAAGGCACCACTTCGTC
CTCACGCCTGATTTATGCAACAACGTCC
CTTTGGTCGCGAGAACATGCGCAAGGATAAGAACGGGACGCAGGAGCCGAAGGCGAAATACCGTGTCAGAAACTGGGCGGCATACAACGCCGGCCTGATC
AATCGAGGCAACATCACGATGTGGATTGGTGACGACGCTCTTGCGAACATGCCCGACACCGAGCCCACACGTGGCCGTTCGCGGCTGTACAGCGATGCAC
TGATTCAGGCATTGCTCGGCTTGAAGACGGTGTTCCATCTGCCGTTGCGTGCCTTGCAAGGCTTCGCGCAAAGCCTGCGTGACCTGGCTTTCGCTGAATT
GCCCGTACCGAACTATACGACCGTGTGCCGTCGCGCACAGGCGCTGCAGGTGAAACTGCCGGTCATTCACAGCGACGAACCGCTGCACTTGGTCGTCGAC
AGCACGGGCGCAAAGGTCTGCGGCGAAGGCGAATGGAAGGTTCGCCAGCATGGCTACTCGAAGCGTCGCACGTGGCGCAAGGTCCATCTCGCACTCGACG
CGAACACGGGCCAGTTGCGAGCGGCGCTCATGACGCACCAGGATGTCGCAGACGGTGAAGTGCTGCCCGAATTGCTTGAACAAATCTCGGCCGACGAGTC
CATCGATACCATCGGCGGCGACGGCGCATACGATACGCAGGCTTGTTACGCCTCGATCACCGCGCGCGGTGCGTTACCCTCGATTCCGCCGCGCGAAGGC
GCGGTTCACTGGCCGACGACCACGCCCGGCGCGTCTCGGCGCAACGCCGCGCTCGACGCCATTGCACAAGGCAGTCGACGCGAATGGAAGCAGCAAAGCG
GTTACCACCGTCGTTCGCTGGCCGAGAACGCGATGTACCGGCTCAAGACGCTCACGGGTCCGTGCCTGTGGGCACGTCGTACCGATACCCAGGCGACCGA
AGTTGCCGTTCGAGTGGGCGTGCTTAACCGCATGGCCGAGCTCTCCCGCCCTCAGTCCGTCCGTATTGCCCGAGATCATGAACAAAGGCACCACTTCGTC
CTCACGCCTGATTTATGCAACAACGTCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 957 bp | 318 aa | 117 | 1073 | + | No |
Chemistry : DDE
ORF sequence :
MRKDKNGTQEPKAKYRVRNWAAYNAGLINRGNITMWIGDDALANMPDTEPTRGRSRLYSDALIQALLGLKTVFHLPLRALQGFAQSLRDLAFAELPVPNY
TTVCRRAQALQVKLPVIHSDEPLHLVVDSTGAKVCGEGEWKVRQHGYSKRRTWRKVHLALDANTGQLRAALMTHQDVADGEVLPELLEQISADESIDTIG
GDGAYDTQACYASITARGALPSIPPREGAVHWPTTTPGASRRNAALDAIAQGSRREWKQQSGYHRRSLAENAMYRLKTLTGPCLWARRTDTQATEVAVRV
GVLNRMAELSRPQSVRIA
TTVCRRAQALQVKLPVIHSDEPLHLVVDSTGAKVCGEGEWKVRQHGYSKRRTWRKVHLALDANTGQLRAALMTHQDVADGEVLPELLEQISADESIDTIG
GDGAYDTQACYASITARGALPSIPPREGAVHWPTTTPGASRRNAALDAIAQGSRREWKQQSGYHRRSLAENAMYRLKTLTGPCLWARRTDTQATEVAVRV
GVLNRMAELSRPQSVRIA
Blast result :
Comments
ISButh7 is 80% aa similar to ISBps3.
There is a ponctual mutation (sequencing error ?) at position 1071, there is a CGA instead of a TGA, this mutation generates the suppression of the STOP codon of the transposase. In this file, the transposase is in silico truncated.
There is a ponctual mutation (sequencing error ?) at position 1071, there is a CGA instead of a TGA, this mutation generates the suppression of the STOP codon of the transposase. In this file, the transposase is in silico truncated.
References
1] ISfinder annotation (2010)
2] Kim,H.S., Schell,M.A., Yu,Y., Ulrich,R.L., Sarria,S.H., Nierman,W.C. and DeShazer,D. (2005) BMC Genomics 6, 174.
2] Kim,H.S., Schell,M.A., Yu,Y., Ulrich,R.L., Sarria,S.H., Nierman,W.C. and DeShazer,D. (2005) BMC Genomics 6, 174.