ISStsp8
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_GG770539 | ND | Streptomyces sp. | Streptomyces sp. SPB74 |
DNA section
IS Length : 881 bp
Ends
IR Length : 17/18
IRL : AGGGCTGTCCCGTAACTGCTGGTCACGGATGAGATGATCATGGCGTGGCT
IRR : AGGGCTGTCCCGTAAATGATCTCCGAGTCGCCTCGGGCGTGGTTGATCGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGATTTACC | TA | GTTGAACTCG | 2 |
| CCTCCTCCGC | TA | GTCTTGTTTT | 2 |
| AGATGACTTC | TA | GGGAGGAGAG | 2 |
DNA sequence
AGGGCTGTCCCGTAACTGCTGGTCACGGATGAGATGATCATGGCGTGGCTGGTGTGATCACGGCGGCGGAGCCGTCCTGGACAGCCCCGTTCACCGGGCT
GAGCCCGCGGCAGTTCGGGAAGCTGGTGACGGTTCTCAGGTGTGAGGGCGCGGACGCCGTCCGCAAGGGCCGGCCGTGGAGCCTTTCGCTGGAGGACCGG
GCCCTGCTGGTCGCGGCCTACTGGCGCACGAATCTGACCATGCGACAGCTCGCCCCGTTGTTTGGGGTGTCCAAGTCGGCGGCGGACCGGATCATCGACC
ACCTCGGACCGATGCTCGCTCTCCAGCCCCGCAAGCGGTTCTCCAGGGACACCGTGCTGATCGTGGACGGCACCCTGGTCCCGACCCGGGATCACACGAT
CGCCGAACGGTCGAAGAACTATCGCTGCTCCACCAACCACCAGGTCGTCATCGACGCCGACACCCGCCTGGCCGTCGTGGTCGGCCGGCCGCTCGCCGGG
AACCGCAACGACCGCGAAGCATGGGAGGAATCCGGCGCCAAGGCCGCCGTCGGCAAGACCCTCACGATCGCCGACGGCGGCTGCCCCGGTACCGGACTCG
TCATCCCTCACCGGCGCGAGCGCGGCCAGACCGAACTTCCGGACTGGAAAGAGGAACACAACAAGTCCCACAAGCAGGTCCGCGCCCGCGTCGAGCATGT
CTTCGCCCGCATGAAGACCTGGAAGATCCTCCGCGACTGCCGCCTCAAAGGAGACGGAGTCCACCACGCCATGCTCGGAATCGCCCGGACGCACAACCTC
ACCCTCGCCGGATAGGGCAGCGGGCCGCACAGCGATCAACCACGCCCGAGGCGACTCGGAGATCATTTACGGGACAGCCCT
GAGCCCGCGGCAGTTCGGGAAGCTGGTGACGGTTCTCAGGTGTGAGGGCGCGGACGCCGTCCGCAAGGGCCGGCCGTGGAGCCTTTCGCTGGAGGACCGG
GCCCTGCTGGTCGCGGCCTACTGGCGCACGAATCTGACCATGCGACAGCTCGCCCCGTTGTTTGGGGTGTCCAAGTCGGCGGCGGACCGGATCATCGACC
ACCTCGGACCGATGCTCGCTCTCCAGCCCCGCAAGCGGTTCTCCAGGGACACCGTGCTGATCGTGGACGGCACCCTGGTCCCGACCCGGGATCACACGAT
CGCCGAACGGTCGAAGAACTATCGCTGCTCCACCAACCACCAGGTCGTCATCGACGCCGACACCCGCCTGGCCGTCGTGGTCGGCCGGCCGCTCGCCGGG
AACCGCAACGACCGCGAAGCATGGGAGGAATCCGGCGCCAAGGCCGCCGTCGGCAAGACCCTCACGATCGCCGACGGCGGCTGCCCCGGTACCGGACTCG
TCATCCCTCACCGGCGCGAGCGCGGCCAGACCGAACTTCCGGACTGGAAAGAGGAACACAACAAGTCCCACAAGCAGGTCCGCGCCCGCGTCGAGCATGT
CTTCGCCCGCATGAAGACCTGGAAGATCCTCCGCGACTGCCGCCTCAAAGGAGACGGAGTCCACCACGCCATGCTCGGAATCGCCCGGACGCACAACCTC
ACCCTCGCCGGATAGGGCAGCGGGCCGCACAGCGATCAACCACGCCCGAGGCGACTCGGAGATCATTTACGGGACAGCCCT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 771 bp | 256 aa | 45 | 815 | + | No |
Chemistry : DDE
ORF sequence :
MAGVITAAEPSWTAPFTGLSPRQFGKLVTVLRCEGADAVRKGRPWSLSLEDRALLVAAYWRTNLTMRQLAPLFGVSKSAADRIIDHLGPMLALQPRKRFS
RDTVLIVDGTLVPTRDHTIAERSKNYRCSTNHQVVIDADTRLAVVVGRPLAGNRNDREAWEESGAKAAVGKTLTIADGGCPGTGLVIPHRRERGQTELPD
WKEEHNKSHKQVRARVEHVFARMKTWKILRDCRLKGDGVHHAMLGIARTHNLTLAG
RDTVLIVDGTLVPTRDHTIAERSKNYRCSTNHQVVIDADTRLAVVVGRPLAGNRNDREAWEESGAKAAVGKTLTIADGGCPGTGLVIPHRRERGQTELPD
WKEEHNKSHKQVRARVEHVFARMKTWKILRDCRLKGDGVHHAMLGIARTHNLTLAG
Blast result :
Comments
ISStsp8 is 95% aa similar to IS112.
References
1] ISfinder annotation (2010)
2] Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D., Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B., Nusbaum,C. and Birren,B. (2009) Direct submission GenBank.
2] Fischbach,M., Godfrey,P., Ward,D., Young,S., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Bochicchio,J., Borenstein,D., Chapman,S.B., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heilman,E.R., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Richards,J., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Thomson,T., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Haas,B., Nusbaum,C. and Birren,B. (2009) Direct submission GenBank.