ISfinder Mirror Site

ISStsp6

Family IS5
Group ISL2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_GG657751	ND	Streptomyces sp.	Streptomyces sp. C

DNA section

IS Length : 1476 bp

Ends

IR Length : 14

IRL : AGGGCTGTCCCGCATTTCCCCCGGGGCAGTGAGCGGCTGACTCGGCCGGG
IRR : AGGGCTGTCCCGCAAATGATCTTCTTGTCGCCTCGGGCGAGGTCGACCGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAGCCTCCGC	TA	GGCTGTCCGC	2

DNA sequence

AGGGCTGTCCCGCATTTCCCCCGGGGCAGTGAGCGGCTGACTCGGCCGGGGCCATACTGTTCGGCTGATGATCAGTTGCGCCTCTGGTCGAGCCGCTGGC
CCCTGTCGATGGTGACCGGCCGCAGCCGGCTCGATCGGTGGCCAGGCTCCTGGAGGTCGATTCCACCGTGTGGACCGAAGCTGTAACGGCCCTGCCCGGA
ACGACGCTCTTTCAGCCGCCTGCATCGGAGCAGTCGCTGGTTCACTGCGCTACGGTCCTCGGCCATCCACTGCCTGCGGACCTCGCAGCCCTCCTCCGGG
AGAGCAACGGCATCGAGGGCGAGTACGGCGACGGGCTCATCTGGTCTGCCGAGCGCATCGCCTCCGAGAACCAGAGTCTTCGCGCAGATGCCGAGCTCGC
CACCTTGTACATGCCGTTCGACCCGCTGCTTTTCTTCGCGGACGCGGGTAACGGGGATTTGTTCGCGCTCTTGGCCAGGATCGACCGGCCCGACGTCTTA
GTCTGGAACCACGAGGACGACAGCCGGACCTGGGTGGCGCCCAGCCTTGCCAAGTACCTCGAATGGTGGTTGACGGGGCGGATCGAGCTGTAGCTCCACG
TCTCATGACCGTCTGGTCGCCAATGAGATGGTCTTGGCGTGGCTGGTGTGATCAAGGCGTCGGAGCCTTCCTGGATAGCCCCCTTCTCCGGGCTGGGTCC
TCGCGTGTTCGGGAAGCTGGTGACGGTTCTGCGGCGCCAGAGTGCGGACGCGGTCCGCAGGGGCCGACCGTGGAGTCTTCCGCTGGAGGACCGGGCACTG
CTTGTCGCTGCGTACTGGCGGACGAATCTCACGATGCGGCAGCTCGCCCCGCTGTTCGGGGTCTCGAAGTCGGCGGCGGGCCGCATCATCGACCACCTCG
GGCCGATGCTCGCTCTCCAGCCCCGCAAGCGGTTCGCCAAGGACACCGTGCTCATCGTCGACGGCACCCTGGTCCCCACACGAGACCACACCATCGCCGA
GCTGTCGAAGAACTGCCGGTACTCCACCAACCACCAGGTCGTTATCGACGCCGACACCCGCCTGATCGTCGTGGTCGGCCGGCCACTCGCCGGAAACCGC
AACGACTGCAAGGCCTGGGAAGAGTCCGGCGCCAAGGCCGCCGTCGGCAAGACCCTCACGATCGCCGATGGCGGCTACCCGGGCACCGGACTCGTCATCC
CTCACCGCCGCGAACGCGGCCAGACCGAACTCCCAGACTGGAAAGAGGAACACAACCGGTCCCACAAACAAGCCCGCGCCCGCGTCGAGCACGTTTTCGC
CCGCATGAAGACCTGGAAGATCCTCCGAGACTGCCGCCTCAAAGGCGACGGCGTCCACCACGCCATGCTCGGCATCGCCCGGATGCACAACCTCGCCCTC
GCCGGATAGGCCAGCGGCCCCGCATAACGGTCGACCTCGCCCGAGGCGACAAGAAGATCATTTGCGGGACAGCCCT

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
426 bp	141 aa	168	593	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MWTEAVTALPGTTLFQPPASEQSLVHCATVLGHPLPADLAALLRESNGIEGEYGDGLIWSAERIASENQSLRADAELATLYMPFDPLLFFADAGNGDLFA
LLARIDRPDVLVWNHEDDSRTWVAPSLAKYLEWWLTGRIEL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
771 bp	256 aa	639	1409	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAGVIKASEPSWIAPFSGLGPRVFGKLVTVLRRQSADAVRRGRPWSLPLEDRALLVAAYWRTNLTMRQLAPLFGVSKSAAGRIIDHLGPMLALQPRKRFA
KDTVLIVDGTLVPTRDHTIAELSKNCRYSTNHQVVIDADTRLIVVVGRPLAGNRNDCKAWEESGAKAAVGKTLTIADGGYPGTGLVIPHRRERGQTELPD
WKEEHNRSHKQARARVEHVFARMKTWKILRDCRLKGDGVHHAMLGIARMHNLALAG

Blast result :

Comments

ISStsp6 orfB (the Transposase) is 95% aa similar to IS112.
The first orf is a passenger gene.

References

1] ISfinder annotation (2010).
2] Fischbach,M., Godfrey,P., Ward,D., Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A.M., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D.I., Hepburn,T.A., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T.D., Shenoy,N., Sisk,P., Stolte,C., Sykes,S.N., Walk,T., White,J., Yandava,C., Straight,P., Clardy,J., Hung,D., Kolter,R., Mekalanos,J., Walker,S., Walsh,C.T., Wieland-Brown,L.C., Galagan,J., Nusbaum,C. and Birren,B. (2009) Direct submission GenBank.