ISfinder Mirror Site

ISStma15

Family IS3
Group IS51

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010943	ND	Stenotrophomonas maltophilia	Stenotrophomonas maltophilia K279a

DNA section

IS Length : 1250 bp

Ends

IR Length : 31/36

IRL : TGGACCGGCCAGGATTTCGTAGACATCTCAGAGCCATAATCAATGGAAGC
IRR : TGGACCGGCCAGACTTCCGTAGACATTCATGAGCCGCCTTGCGCGTAGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGTGATAGAG		AAGCCAGTGC	0

DNA sequence

TGGACCGGCCAGGATTTCGTAGACATCTCAGAGCCATAATCAATGGAAGCGAGAGAGGTGTCTAATGGCAAGCAAACAGTACACGGATGAGTTCAAGGCC
GAGGCGGTCAAGCAGGTTACCGAAAGAGGCTTTGCGGTAGCCGAGGTTGCCAAGCGGTTGGGGGTCTCGTCCCACAGCCTTTACCAATGGCTCCGCAAGT
CCAGGAGCGAGGCCTTGGCGGGCACGCCTGAGGGGTCGATGTTGCCGACGCCGAAGGACTCAGCCGAGGTGCGGCGCCTGAAGGCCGAGGTGAAACGCCT
GACTGAGGAGCGCGACATCCTAAAAAAAGCCGCGGCGTACTTCGCCAAGGGATGACGGCGAAGTACGCTTTCATGCGTGAGCACTTGGGCGAGTTCAGGC
TGACGGCCATGTGTCGAGTGCTTGGGGTTAACCGCAGTGGCTACTACGCATGGGCGGGCAATTCGGGCAGGCCGCGTCGTCGTGAGGATGATCGTCTGCG
CGGCCTTATCAAGCACGCGTGGTTGGCAAGCGGGACGGTGTACGGCTACCGCAAGATCACCCGGGAACTTCGTGAGGCCGGTGAACGTTGCAGCCGCCAT
CGTGTTCGACGTCTGATGAAGGCAGAGGGCATAAGGGCAGAAATTGGCTACGGCACCAAGCCGCGCCATCGAGGCGGTCCGCCTGGGATGGTTGAAAACG
TCGTCAATCGCGACTTTTCGCCCGCTGCACCAAACAAGGTGTGGGTCACTGACATAACATACATCCGCACCTACGAGGGCTGGACTTTCCTGGCAGTGAT
CGTCGACCTTTACTCACGGCAAGTGGTTGGCTGGGCGATGCAGTCCCAGATGACCACCGACCTTGTGCTGCAGGCGCTGGTATCGGCGGTGTGGAAGCGA
AAGCCTGCTGCGGGCCTGATCATCCACTCTGACCAAGGCAGCCAGTTCACGAGCAGCGACTGGCTATCGTTGCTGAAGCAGAATGGCATGGTTCCAAGCA
TGAGTCGGCGTGGAAACTGCCATGACAATGCGGTGGCTGAGAGCTTCTTCAGTGCACTGAAGAAGGAGCGGATCAAGCGACGGATCTACCCGACCCGGGA
CGAGGCGCGCTCGGATGTATTCAATTACATCGAGATGTTTTACAACCCAATCCGACGCCACGGTTCCGCTGGCAACCTGGCCCCTGTAGAGTTTGAACGG
CGCTACGCGCAAGGCGGCTCATGAATGTCTACGGAAGTCTGGCCGGTCCA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
291 bp	96 aa	65	355	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MASKQYTDEFKAEAVKQVTERGFAVAEVAKRLGVSSHSLYQWLRKSRSEALAGTPEGSMLPTPKDSAEVRRLKAEVKRLTEERDILKKAAAYFAKG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
918 bp	305 aa	307	1224	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GARHPKKSRGVLRQGMTAKYAFMREHLGEFRLTAMCRVLGVNRSGYYAWAGNSGRPRRREDDRLRGLIKHAWLASGTVYGYRKITRELREAGERCSRHRV
RRLMKAEGIRAEIGYGTKPRHRGGPPGMVENVVNRDFSPAAPNKVWVTDITYIRTYEGWTFLAVIVDLYSRQVVGWAMQSQMTTDLVLQALVSAVWKRKP
AAGLIIHSDQGSQFTSSDWLSLLKQNGMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYPTRDEARSDVFNYIEMFYNPIRRHGSAGNLAPVEFERRY
AQGGS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1160 bp	386 aa	65	1224	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MASKQYTDEFKAEAVKQVTERGFAVAEVAKRLGVSSHSLYQWLRKSRSEALAGTPEGSMLPTPKDSAEVRRLKAEVKRLTEERDILKKSRGVLRQGMTAK
YAFMREHLGEFRLTAMCRVLGVNRSGYYAWAGNSGRPRRREDDRLRGLIKHAWLASGTVYGYRKITRELREAGERCSRHRVRRLMKAEGIRAEIGYGTKP
RHRGGPPGMVENVVNRDFSPAAPNKVWVTDITYIRTYEGWTFLAVIVDLYSRQVVGWAMQSQMTTDLVLQALVSAVWKRKPAAGLIIHSDQGSQFTSSDW
LSLLKQNGMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYPTRDEARSDVFNYIEMFYNPIRRHGSAGNLAPVEFERRYAQGGS

Blast result :

Comments

ISStma15 is 85% aa similar to ISBcen21.
ISStma15 is disrupted by ISStma11.

References

1] ISfinder annotation (2010).
2] Crossman,L.C., Gould,V.C., Dow,J.M., Vernikos,G.S., Okazaki,A., Sebaihia,M., Saunders,D., Arrowsmith,C., Carver,T., Peters,N., Adlem,E., Kerhornou,A., Lord,A., Murphy,L., Seeger,K., Squares,R., Rutter,S., Quail,M.A., Rajandream,M.A., Harris,D., Churcher,C., Bentley,S.D., Parkhill,J., Thomson,N.R. and Avison,M.B. (2008) Genome Biol. 9 (4), R74.
3] Crossman,L.C. (2007) Direct submission GenBank.