ISCaa9
- Family IS5
- Group IS1031
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010830 | ND | Candidatus Amoebophilus | Candidatus Amoebophilus asiaticus 5a2 |
DNA section
IS Length : 871 bp
Ends
IR Length : 16
IRL : GAGGCTGTCTAAAAAGGGTAATTTAGATAGGAAAAGCAGACAAAACTTTA
IRR : GAGGCTGTCTAAAAAGCCAAAATGGAGTCATAAAAGCCGCTTAAGCATGA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TGCGACCGTC | TTA | AAGCAAAGCG | 3 |
GCAGAAACTC | TTA | GACACTTTAG | 3 |
CTTATTGCTC | TTA | GACCTTGTTG | 3 |
CCCTAATGAC | TTA | GAGGATAAAT | 3 |
TGAATGGCTC | TAA | GAGTGCCAGC | 3 |
TTATCTGCTC | TAA | GAGAACGTTT | 3 |
ACAATAGTTT | TTA | TAGCTAGTTG | 3 |
TGGTGACCTC | TTA | TAGCAGCAAT | 3 |
AGCCAGCTTC | TAA | GAGATACCCA | 3 |
GAAACTCTAA | TTAGAGCAAC | 0 | |
TGAACTCTTA | TAAGAAAACT | 0 | |
TAATCTCTAA | TTAGGGTTGA | 0 | |
AAAACTCTAA | TTACCTAAAG | 0 | |
ATTGCTCTTA | TAAGAGGTTA | 0 | |
TAACCTCTTA | TGAGAGGTTT | 0 |
DNA sequence
GAGGCTGTCTAAAAAGGGTAATTTAGATAGGAAAAGCAGACAAAACTTTATAAAATTAGTTAGTGAACTTTAAACTAACAGCTATGCCATACACTAGCGA
TTTAACAGATAAGGAATGGGAAGTGTTAGAACCATTAGTTACTTACATAGGTCCTTGCCGACCCCGTAAATACACTATAAGAGAAGTATTAGATGCTATT
TTCTATTTAGAAAAAACAGGCTGTCAATGGCGCATGCTACCTGCCCATTTTCCACCCTGAAAAAGTGTTTATGACTTGTATTATCGCTGGCGAAGAAATG
GTAAATGGGACTTAGTCCATGATACACTTCGAGATCAAGTACGAGAAAAGTTGGGCAAACAAGCCATACCTAGCGCTGCCATTATGGATAGCCGCTCGGT
TAAAACAGCACAAAAAGGGGGTCGAGAGGCTATGATGCAGCAAAAAAAGTAAAAGGCCGTAAGCAGCATATTATTGTTGATACGCTGGGGCTTATACTTT
GTGTGGTAGTACATTCAGCTCACTTATCAGACTCAAGAGGATGTCATCTTTTGCTTATTCGCCTTTTTAATACATTCCCTTCTATTAAACACATATGGGT
AGATGGAGGATACCAGAAAGGATGTATAATGTGGGCTTATGGGGTGCTAGGTTATACTTTAGAAGTTGTACAACGGCTCTCAAATGGGTTTCATATTCTA
AAAAAGCGATGGATAGTCGAGCGTACATTTGCTTGGCTGAGTTTTTCTAGGAGGCTCTCTAAAGATTACGAGCATAATCCCAAGTCTTCTGAAACACATA
TTAAAATTGTTATGATTAGAATCATGCTTAAGCGGCTTTTATGACTCCATTTTGGCTTTTTAGACAGCCTC
TTTAACAGATAAGGAATGGGAAGTGTTAGAACCATTAGTTACTTACATAGGTCCTTGCCGACCCCGTAAATACACTATAAGAGAAGTATTAGATGCTATT
TTCTATTTAGAAAAAACAGGCTGTCAATGGCGCATGCTACCTGCCCATTTTCCACCCTGAAAAAGTGTTTATGACTTGTATTATCGCTGGCGAAGAAATG
GTAAATGGGACTTAGTCCATGATACACTTCGAGATCAAGTACGAGAAAAGTTGGGCAAACAAGCCATACCTAGCGCTGCCATTATGGATAGCCGCTCGGT
TAAAACAGCACAAAAAGGGGGTCGAGAGGCTATGATGCAGCAAAAAAAGTAAAAGGCCGTAAGCAGCATATTATTGTTGATACGCTGGGGCTTATACTTT
GTGTGGTAGTACATTCAGCTCACTTATCAGACTCAAGAGGATGTCATCTTTTGCTTATTCGCCTTTTTAATACATTCCCTTCTATTAAACACATATGGGT
AGATGGAGGATACCAGAAAGGATGTATAATGTGGGCTTATGGGGTGCTAGGTTATACTTTAGAAGTTGTACAACGGCTCTCAAATGGGTTTCATATTCTA
AAAAAGCGATGGATAGTCGAGCGTACATTTGCTTGGCTGAGTTTTTCTAGGAGGCTCTCTAAAGATTACGAGCATAATCCCAAGTCTTCTGAAACACATA
TTAAAATTGTTATGATTAGAATCATGCTTAAGCGGCTTTTATGACTCCATTTTGGCTTTTTAGACAGCCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
369 bp | 122 aa | 84 | 452 | + | No |
Description : First part of the transposase
ORF sequence :
MPYTSDLTDKEWEVLEPLVTYIGPCRPRKYTIREVLDAIFYLEKTGCQWRMLPAHFPP*KSVYDLYYRWRRNGKWDLVHDTLRDQVREKLGKQAIPSAAI
MDSRSVKTAQKGGREAMMQQKK
MDSRSVKTAQKGGREAMMQQKK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
441 bp | 146 aa | 404 | 844 | + | No |
Description : Second part of the transposase
ORF sequence :
NSTKRGSRGYDAAKKVKGRKQHIIVDTLGLILCVVVHSAHLSDSRGCHLLLIRLFNTFPSIKHIWVDGGYQKGCIMWAYGVLGYTLEVVQRLSNGFHILK
KRWIVERTFAWLSFSRRLSKDYEHNPKSSETHIKIVMIRIMLKRLL
KRWIVERTFAWLSFSRRLSKDYEHNPKSSETHIKIVMIRIMLKRLL
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
761 bp | 253 aa | 84 | 844 | + | Yes |
Chemistry : DDE
ORF sequence :
MPYTSDLTDKEWEVLEPLVTYIGPCRPRKYTIREVLDAIFYLEKTGCQWRMLPAHFPP*KSVYDLYYRWRRNGKWDLVHDTLRDQVREKLGKQAIPSAAI
MDSRSVKTAQKGGREAMMQQKKVKGRKQHIIVDTLGLILCVVVHSAHLSDSRGCHLLLIRLFNTFPSIKHIWVDGGYQKGCIMWAYGVLGYTLEVVQRLS
NGFHILKKRWIVERTFAWLSFSRRLSKDYEHNPKSSETHIKIVMIRIMLKRLL
MDSRSVKTAQKGGREAMMQQKKVKGRKQHIIVDTLGLILCVVVHSAHLSDSRGCHLLLIRLFNTFPSIKHIWVDGGYQKGCIMWAYGVLGYTLEVVQRLS
NGFHILKKRWIVERTFAWLSFSRRLSKDYEHNPKSSETHIKIVMIRIMLKRLL
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
In the orfA, there is a proposed stop codon readthrough at position 258.
ISCaa9 is present in 15 identical copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
ISCaa9 is 64% aa similar to ISMac15.
In the orfA, there is a proposed stop codon readthrough at position 258.
ISCaa9 is present in 15 identical copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
ISCaa9 is 64% aa similar to ISMac15.
References
1] Schmitz-Esser S, Tischler P, Arnold R, Montanaro J, Wagner M, Rattei T, Horn M. (2010) J Bacteriol., 192(4):1045-1057.