ISfinder Mirror Site

ISCsa9

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009437	ND	Caldicellulosiruptor saccharolyticus	Caldicellulosiruptor saccharolyticus DSM 8903 Caldicellulosiruptor lactoaceticus Anaerocellum thermophilum Caldicellulosiruptor obsidiansis

DNA section

IS Length : 2475 bp

Ends

IR Length : 31/52

IRL : TGTTAATGATAAAATAAAATCATCAGAAATGACACTAAAAATTCCCCACC
IRR : TGTTATTGTCAATACCTTTTCCCCACTTTTGATAATATTTTTTCCCCACC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGGGATGTTT	CAGATAG	TTTTTTTCCA	7
GATGGCAATG	ACTAAT	TTTATTAAAA	6
TTAATCTTTT	CTCGC	TGGTTATTAA	5

DNA sequence

TGTTAATGATAAAATAAAATCATCAGAAATGACACTAAAAATTCCCCACCTGCCACTTAAAAAGAAGCCTTCTGTAGTGTAGAATGGAAAGTATGGGAAA
ACTACAGAAGGAGAAGATAACAAGAAAATGTTGGGGGTTGCAATGCACACAACAATCTACACACTTTTCAAACGGGGATACAACAAAAGTCAAATAGCAA
GATTGTTAGACGTGGATAGAAAAACTGTTAGAAAAGTAATCCATGACATTGAACAGAAAGGAGAAGTCGAGAGAAAATCAAAAGGTTCTGTATTAGATAA
TTACAGGGAGTTCATTGAGGTAAAGGTTAATAAAGGACTTTCAGCAAAGAAAATACATCAAGACTTGAAAGCGGAATTTGGTTTTGAAGGAAGCTACTCG
AATGTAAGAAGATATGTCCAAAAGGTAAAACAAAAGATAGCAAATTCAAAGGTGTACATGGTTTTAACAACACTGCCTGCAGAAGAAGCTCAAGTTGATT
TTGGATATATAGGCAAGATAAAAGTTGATGGCAAATTCAAAAAAGCATGGGTATTTACAATGGTTTTAAGTTATTCAAGATATATGTATGCAGAGATAGT
ATTTGAGCAGACAGTTGAAACATTTATACAGTGTCATAAGAACGCATTTAAGTATTTTGGTGGGGTAGTAGAAGTTGTAAAGATAGACAACTTAAAAGCA
GGTGTATTGAATGTTGATTTTTATGAGGCGCAAATACAAAAAGATTATGCAAGTTTTGCAAGCCACTATGGATTTTTACCTCAGCCATGTAGGGTATACA
CACCGACTGATAAAGGCAAAGTAGAATCAGCAATTAAGTATGTTAAGCAAAATTGTTTTTCTGGGGAAGAATTTAAGGATATTGATGAGGCGAGGGAGTA
TTTAAAAAACTGGCTTGACAATGTAGCAAATGTGAGAGTACATGGCACAACCAAGAAAGTTCCCAAAGAAGTTTTCATCTCAGAAGAGAAAGAGAAGTTA
ATAGCTCTTCCTGTTGAGGAATATTACATATCAAGAAGTTCAATTCATAAAGTAGCTACTAACTGTCATCTCATATACAAAGGGAACTATTATTCAGTGC
CATATGAGTATGCAGGAGGTGAAGTAGAGGTAGTTGAGATTGGCAGTTTTTTGAGGGTGTTCTTTGAGGGTAAAGAAATAGCCCTTCATCAGATTGTCAA
AAACAATGAGAAGGGCAAATACGTAACCAACAAAGAACACTATCCCTCGTCTAAGAATATAACAATTGAGGATATAATGTCAAGACAGAGGGAGCAAATG
GCAGAAATTGGGGAGTGGGCGTTGAAGTTTTTTGAAGAATTTACCGAGCAAGAAGGATTTAAGAAGTATGACTACAGAAGCATAAGTGGGATAATAGCAT
TAAAAGAAAGATATGGATCAAAGATGGTAGACAATGCGTGTAAGAGGGCTTTAAAATTCAGAGGATTGAGTTACAAGCTTGTGAAGAATATATGTGAAAA
AGGGATAAGCGATTTACCTGAGTATGAAGATGAGAGCTATATTAATGAGGAAAGAACAGAGCTTTACAGGGATATCAGGGAATATGACAAATTGCTTGAG
ATAGGAGAATTGCAAAGATGAATGATCTTTTGTTGGGGAAGTTAAAGGATTTGAAATTATCCGGGATAATAAAAAGTTTTGATTTAAGAGTAGAGGAAGC
TATTAAGAATAACTTTTCATATCAAGAGTTTTTTGAGATATTGATAAATGATGAAGTGAGTAACAGGAGAATAAACAGTAATCAAAAGAGGATAAGCAAA
GCGAAGTTTCCATGGCACAAGACGTTAGAAGAATACAATTTTAATTATCAGCCTTCAATTAATAAAAGGTTTATATACAATTTGGCGACCTGTGAATTTG
TCCGAAAAAGGGAGAATGTGGCCTTCATAGGACCGCCAGGGACAGGGAAGACACATCTTGCAATAGCTATAGGACTTAAAGCTGTAGCACTTGGATATAG
AGTTTTGTTTACCACAGCAAATGAGATGTTAGAAGAGTTGTATATTTCAAGAGCGGATAATTCGTATCAACAAAAGCTAAAAAACTATGTTAATGTGGAT
TTGTTAATAATAGATGAGCTGGGCTTAAGGAAATTTAATCAAAGCAGTGTAGATGATTTTTATGAGATAATATCAAAGAGATATGAGAGAGGATCGATAA
TAATAACCACAAACAAAGTATTTGAAGAGTGGGCGAGGATATTTTATGATCCAGTTTTAGCGACAGCGATTTTAGATAGATTTGTACATCATTGTCATTT
TGTGGTTATCAAAGGTGAAAGTTATAGGATGAAGCAAAGGGAGGGTGCTATAAAAGCTTTAACATATGATTCCAAGAATGACTCAAATCAGTTAAATAAT
GATAATTAAATTTTTTTTGAAAACAGGTGGGGAAAAAATATTATCAAAAGTGGGGAAAAGGTATTGACAATAACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1479 bp	492 aa	143	1621	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MHTTIYTLFKRGYNKSQIARLLDVDRKTVRKVIHDIEQKGEVERKSKGSVLDNYREFIEVKVNKGLSAKKIHQDLKAEFGFEGSYSNVRRYVQKVKQKIA
NSKVYMVLTTLPAEEAQVDFGYIGKIKVDGKFKKAWVFTMVLSYSRYMYAEIVFEQTVETFIQCHKNAFKYFGGVVEVVKIDNLKAGVLNVDFYEAQIQK
DYASFASHYGFLPQPCRVYTPTDKGKVESAIKYVKQNCFSGEEFKDIDEAREYLKNWLDNVANVRVHGTTKKVPKEVFISEEKEKLIALPVEEYYISRSS
IHKVATNCHLIYKGNYYSVPYEYAGGEVEVVEIGSFLRVFFEGKEIALHQIVKNNEKGKYVTNKEHYPSSKNITIEDIMSRQREQMAEIGEWALKFFEEF
TEQEGFKKYDYRSISGIIALKERYGSKMVDNACKRALKFRGLSYKLVKNICEKGISDLPEYEDESYINEERTELYRDIREYDKLLEIGELQR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
792 bp	263 aa	1618	2409	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNDLLLGKLKDLKLSGIIKSFDLRVEEAIKNNFSYQEFFEILINDEVSNRRINSNQKRISKAKFPWHKTLEEYNFNYQPSINKRFIYNLATCEFVRKREN
VAFIGPPGTGKTHLAIAIGLKAVALGYRVLFTTANEMLEELYISRADNSYQQKLKNYVNVDLLIIDELGLRKFNQSSVDDFYEIISKRYERGSIIITTNK
VFEEWARIFYDPVLATAILDRFVHHCHFVVIKGESYRMKQREGAIKALTYDSKNDSNQLNNDN

Blast result :

Comments

ISCsa9 orfA (the Transposase) is 49% aa similar to IS1631, and orfB (helper of transposition) is 66% aa similar to ISChy4.

References

1] Land M. (2010) Direct submission.
2] van de Werken,H.J., Verhaart,M.R., VanFossen,A.L., Willquist,K., Lewis,D.L., Nichols,J.D., Goorissen,H.P., Mongodin,E.F., Nelson,K.E., van Niel,E.W., Stams,A.J., Ward,D.E., de Vos,W.M., van der Oost,J., Kelly,R.M. and Kengen,S.W. (2008) Appl. Environ. Microbiol. 74 (21), 6720-6729.
3] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P. (2007) Direct submission GenBank.