ISfinder Mirror Site

ISGeob6

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013757	ND	Geodermatophilus obscurus	Geodermatophilus obscurus DSM 43160

DNA section

IS Length : 912 bp

Ends

IR Length : 15/17

IRL : GAAGACGTTGCAGAAGTAGGTCTTCGGGCTCTGTTCCGCTCGAGCGGTTC
IRR : GAAGACGTTGCATCAGTTCGCGGGTAGGCGTCGGGCGCAGATCAGGGTGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGCCGAGCAA		TGAGAGTCTT	0

DNA sequence

GAAGACGTTGCAGAAGTAGGTCTTCGGGCTCTGTTCCGCTCGAGCGGTTCGGCGCAGGGTGCCCCCCATGTCGTCGGTGTCTTCCCCGCCACGGCTGATA
AGTGACGAGCTGTGGGCGCTAGCCGAGCCGCTGATCCCGCCACAGAGGCCGGCGGTGCACGGGCGCACCGGTCGACCCCGGGTCTTCGACCGGGACGTGC
TCGAAGGCGTCGCCTTCGTGCTCTCCACCGGCATCGGCTGGCCCAAGCTGCCCCGCGAACTGGGTTACGGCTCGGGTTGGACCTGCTGGCGGCGGATGCA
CGAGTGGGCCGAGGCCGGCGTCTTCGACCAGCTGCATACCGCCGTGCTTGACCGACTCGGCGAGAGTGGCCGGCTGGACTGGTCGCGGGCCAGCCTGGAC
TCGGTCAGCGTCCGGGCGAAAAGGGGGGTGAGCTGACCGGGCCGAACCCGACCGACCGAGGCAAGGCCGGCAGCAAGTACCACCTGCTCGTCGACGCCAC
CGGGCTACCGCTCAACGTCCTGTTCTCCGGCGCGAACCGGCACGACAGCCTCTTCGTCGAACCCATCCTCGACCCGATGCCGGCGATCAAGCGAGGCGGC
CGCGGCCATCCCCGCCGCCGGCCGATCAAGCTGCACGGCGACAAGGGCTATGACAACCCGCGGGTGCGCCGCTATCTGCGCCACCGCGGGATCACCGCCC
GGATCGCCCGAATCGGCCGAATCGGCCGCGACTCCAGCGCGCGACTGGGCCGCCACCGCTGGGTCGTTGAGCGCACCCTCGGCTGGCTGCTGTCCTACAA
ACGCCTGGCTCTGCGCTACGACCGCACCGCCCTCACCATCACCGCGCTGGTCCGCCTGGCCGTCACCCTGATCTGCGCCCGACGCCTACCCGCGAACTGA
TGCAACGTCTTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
369 bp	122 aa	68	436	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSSVSSPPRLISDELWALAEPLIPPQRPAVHGRTGRPRVFDRDVLEGVAFVLSTGIGWPKLPRELGYGSGWTCWRRMHEWAEAGVFDQLHTAVLDRLGES
GRLDWSRASLDSVSVRAKRGVS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
549 bp	182 aa	352	900	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PTRREWPAGLVAGQPGLGQRPGEKGGELTGPNPTDRGKAGSKYHLLVDATGLPLNVLFSGANRHDSLFVEPILDPMPAIKRGGRGHPRRRPIKLHGDKGY
DNPRVRRYLRHRGITARIARIGRIGRDSSARLGRHRWVVERTLGWLLSYKRLALRYDRTALTITALVRLAVTLICARRLPAN

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
833 bp	277 aa	68	900	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSSVSSPPRLISDELWALAEPLIPPQRPAVHGRTGRPRVFDRDVLEGVAFVLSTGIGWPKLPRELGYGSGWTCWRRMHEWAEAGVFDQLHTAVLDRLGES
GRLDWSRASLDSVSVRAKKGGELTGPNPTDRGKAGSKYHLLVDATGLPLNVLFSGANRHDSLFVEPILDPMPAIKRGGRGHPRRRPIKLHGDKGYDNPRV
RRYLRHRGITARIARIGRIGRDSSARLGRHRWVVERTLGWLLSYKRLALRYDRTALTITALVRLAVTLICARRLPAN

Blast result :

Comments

ISGeob6 is 90% aa similar to ISGeob5.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.

References

1] ISfinder annotation (2010)

2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Munk,A.C., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,M., Schneider,S., Klenk,H.-P. and Eisen,J.A. (2010) Direct submission GenBank