ISfinder Mirror Site

ISBugl2

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012724	ND	Burkholderia glumae	Burkholderia glumae BGR1 chromosome 2 Burkholderia glumae BGR1 chromosome 1

DNA section

IS Length : 914 bp

Ends

IR Length : 17

IRL : GAGGCCAGTTCAAAAACTCCCGAGAGGGGCTGTTTACTTCCTCGGCAAGC
IRR : GAGGCCAGTTCAAAAACCCTGCTCAGCTCGTCTGAAGATGTTCCAACAGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGTACCCGAC	TTA	GCGAGAACCG	3
GTAGCCTTCT	TTA	AAATGGCCTT	3
TCTTTTCCGC	TAA	GGATGACGCC	3
TGTTGGCCGT	TAA	GAGCCCGGAG	3
CGTGTTGCGC	TAA	GAAACGGTTT	3
TTATTGATTT	TTA	CGACGATGTA	3
TGGTTCCTTA		TAAGAGAACA	0

DNA sequence

GAGGCCAGTTCAAAAACTCCCGAGAGGGGCTGTTTACTTCCTCGGCAAGCTGTTAACCTTGGGCATCAGAACAACGGAAGTCCAAGGATGGAAGCGCCAA
TCATTGACGACGAACTGTGGATACTGATCGAACCATTGCTGCCACCTGCGAAGCTTCGAGCGAAGAGCGATCCTGGTCGCCCGCGCGTGTCCGACCGTGC
GGCTCTCAACGGCATCCTGTTCGTGTTCAAAACGGGAATTCGTTGGAACCACTTGCCGACTCGTCTGGGCTTTGGCTCGGGCGCCACATGCTGGCGGCGA
TTGAGCGACTGGCAAAAGGCTGGTGTATGGGACCAACTGCACGAGTTGCTACTCGACAAGTTGCGTGCGGCCGGCCAAATCGATCTGTCATACGCTGCGG
TCGATTCGTCGTCCGTGCGCGCCGTTGGGGCGGGCGAAAAACTGGCCCGAACCCCACCGATCGCGCGCGACCCGGTTCCAAGCACCACGTCCTCGTAGAC
GCCAACGGCGTTCCTCTCGTTGCGATCCTGACTGGCGCGAACACCAACGACGTCACGCAGTTGCTGCCGCTCGTTGATGCGATTCCACCCATTCGCGGCG
TTCGTGGCCGACCGCTTCAGAAGCCCGGCGTCGTCTACGCCGATCGCGGCTACGATTCCACCCGACATCGTCGCGCGTTGCGCGAACGCGGTATCAAGCC
CGTGATCGCCAAGCGCCGGACCGAGCATGGCAGTGGTCTGGGCAAGTATCGTTGGGTGGTCGAACGTACGCATTCCTGGCTCCACAATTTCCGGCGCCTA
CGCACTCGCTTCGAGCGAAGTGCCTACATTCACGAAGCATTCCTCAAACTTGGCTGCTCGATCATCTGTTGGAACATCTTCAGACGAGCTGAGCAGGGTT
TTTGAACTGGCCTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
411 bp	136 aa	88	498	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MEAPIIDDELWILIEPLLPPAKLRAKSDPGRPRVSDRAALNGILFVFKTGIRWNHLPTRLGFGSGATCWRRLSDWQKAGVWDQLHELLLDKLRAAGQIDL
SYAAVDSSSVRAVGAGEKLARTPPIARDPVPSTTSS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
606 bp	201 aa	300	905	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IERLAKGWCMGPTARVATRQVACGRPNRSVIRCGRFVVRARRWGGRKTGPNPTDRARPGSKHHVLVDANGVPLVAILTGANTNDVTQLLPLVDAIPPIRG
VRGRPLQKPGVVYADRGYDSTRHRRALRERGIKPVIAKRRTEHGSGLGKYRWVVERTHSWLHNFRRLRTRFERSAYIHEAFLKLGCSIICWNIFRRAEQG
F

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
818 bp	272 aa	88	905	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEAPIIDDELWILIEPLLPPAKLRAKSDPGRPRVSDRAALNGILFVFKTGIRWNHLPTRLGFGSGATCWRRLSDWQKAGVWDQLHELLLDKLRAAGQIDL
SYAAVDSSSVRAVGAGEKTGPNPTDRARPGSKHHVLVDANGVPLVAILTGANTNDVTQLLPLVDAIPPIRGVRGRPLQKPGVVYADRGYDSTRHRRALRE
RGIKPVIAKRRTEHGSGLGKYRWVVERTHSWLHNFRRLRTRFERSAYIHEAFLKLGCSIICWNIFRRAEQGF

Blast result :

Comments

ISBugl2 is 88% aa similar to ISJp4.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.

References

1] ISfinder annotation (2010).

2] Lim,J., Lee,T.H., Nahm,B.H., Choi,Y.D., Kim,M. and Hwang,I. (2009) J. Bacteriol. 191 (11), 3758-3759

3] Hwang,I., Lim,J., Lee,T.H., Nahm,B.H., Choi,Y.D. and Kim,M. (2009) Direct submission GenBank