ISfinder Mirror Site

ISUnCu14

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AB266146	ND	uncultured bacterium	uncultured bacterium

DNA section

IS Length : 2442 bp

Ends

IR Length : 21/25

IRL : GTAAGCGATGTTCTGCTCCCACCTTCTCTGGCGTGTTCTGATCTGGGATC
IRR : GTAAGCGGTGTTCCGATCACACCTTGGCGGCGTAGTTCCACGGCAAGAGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCTCAGCGAACC	GTTGATTA	CAAATCTGCC	8

DNA sequence

GTAAGCGATGTTCTGCTCCCACCTTCTCTGGCGTGTTCTGATCTGGGATCGGGATGTCCTGTGATGGGCGGGAGGAAGTTTCTCCATGGCGGCTACATTG
GAGGTTCTCCCGCTCGGTTGCGGCGGTCGCCACCGGCGGACGTGGCCGGATGAGGTGAAGGCGCGGATCGTGGCGGAGACGCTGCGCCCGGGTGTGACGG
TGAACGAGGTCGCGGCACGGCACGGGCTGCAGGCCAACCATCTGTCGTCGTGGCGGTCGCTCGCCCGGGCGGGAAAGCTGGTGCTGCCTGCACCGGAGGG
TTCGGTCGAGTTCGCGGCCATGATCGTGGCACCCCCGGGCGAGTGCCCGCGGCGTTCCGAAGCCGTCGGCCCGGAGATCATGGTCGGTGCCGTGACGGTC
CGCCTTGAGGCCGGCGCCCCGGTCGAACGGATTGCGACCCTCGTGCGCGCGCTGTCGGGCCCGGCATGATCTTCCCGTCGCATCGGGTGCGGATCATGGT
GGCGACGAAGCCCATCGATTTCCGCAAGGGCCACGACAGCCTGGCGGCGTTGGTGAAGAACGAACTGCGCAAGGACCCGTTCACCGGGACGGTATTCGTG
TTCCGCGCCCGCAAGGCGGACCGGCTGAAGCTGCTTTACTGGGATGGCACCGGGCTGGTGATGGCCTACAAGCGGCTGGAGGAGCACAGCTTCAGCTGGC
CCCCGGTGAAGGACGGCCTGATGACGCTGAGCCACGCCCAGTTCGAGGCACTGTTCGCCGGGCTCGACTGGCGGCGGGTCCGGGCCGTCGAGGCCCGCGC
GCCCGAGGCGGTGGAGTGACTGCGACAGGATGACTCGGGTGGTGCTTTCGGCGGGCGCCCGGAGGGCGGATCTGATAGACCGCGCTCATGCTGGCCCCTG
CCGATCTGCCTGACGATATTGCTGCCCTGAAGGCGATGCTGATCGCCTCGGAGGCGCGCAACAATCGCAAGGATGAGCGGATCGAACGGCTGGAGAAGCT
GGTCGCGGCGTTCCGGCAGGCGGCCTTCGGGCGGCGCTCGGAGAAGAGCGATCCGGGCCAGTTCGAACTGGCACTCGAGGATCTGGAGACCGCCATCGCC
GCGATCCGTGCCGAAGAGGATGCCGAGGATCGCGCGGCGAAGCGCCCGTTCAGGCCGCGCGCCATCAATCGCGGATCGCTGCCCAGGCACCTGCCACGCA
TCGACGAGGTCATCGAGCCTGAGAGCCTGACCTGCGCCTGCGGCGGTTGCCTGCATTGCATCGGCGAGGACGTGTCGGAACGGCTGGACATCGTTCCCGC
TCAGTTCCGCGTCATCGTCACCCGTCGTCCGAAGTATGCCTGCCGCTCCTGCACCGACGGCGTGGTGCAGGCCCCGGCGCCGGCGCGGCTGATCCCGGGC
GGCATGCCGACCGAGGCGACGGTCGCCCATGTGCTGGTCAGCAAATACGCCGACCACCTTCCGCTTTACCGCCAGGCGCAGATTTACAGCCGCCAGGGCA
TCGATCTCGACCGGTCCACCCTGGCCGACTGGGTCGGCCGCGCCGCCTTCGAGCTGCGCCCGGTCTTTGACGCCCTGATGGCCGACCTGAAGCGGTCGAC
GAAGCTGTCCATGGACGAGACCCGCGCCCCGGTCCTCGATCCGGGGGCACGGAAGACGAAGACCGGATACTTCTGGGCACTGGCCCGCGATGATCGGCCC
TGGGGCGGCACCGCGCCGCCAGGCGTGGCCTTCACCTATGCTCCGGGTCGAGGCGGGCAGCACGCCGAACGGATCCTGCAGGGCTTCGGTGGCATCCTGC
AGGTCGATGGATACGCAGGTTACAACCGGCTGATCGCGCCCGACCGGGTCGGCCCAGGCATCCAACTGGCCTATTGCTGGGCCCATGCGCGCCGAAAGCT
CATAGAGATCACCCGCACCGGGCCCGCACCGATCGCCGAGGAGGGCGTCGACCTCATCCGCGATCTCTATCGTATCGAGGCTGACATTCGCGGCAGCGAC
CCCACCGCCCGCCTGGTCGCGCGGCAGGACCGTTCAGCCCCGATCCTCGCCCGCCTCGACGACTGGCTGTGCCATCACCGCGCCCGCGCGTCCGCAAAGT
CGCCACTGGGCGAGGCGCTCGCCTACATCGCCAGATACCGTGACGGCCTTGGACGTTTCCTGACCGATGGCCGCATCGAGATCGACTCCAATGCCGTCGA
ACGCACCATCCGTCCGATCGCGCTGAACCGGAAGAATGCCCTCTTCGCCGGGCACGACACCGGCGCCGAAAACTGGGCCGTCATTGCCTCGCTGATCGAG
ACCTGCAAACTCAACAGCGTCGATCCCCAGACCTGGCTGGCGAACACGCTCACCGCCATAGCCAATGGGCATAAGCAGAGCCAAATCAACGATCTCTTGC
CGTGGAACTACGCCGCCAAGGTGTGATCGGAACACCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
384 bp	127 aa	86	469	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MAATLEVLPLGCGGRHRRTWPDEVKARIVAETLRPGVTVNEVAARHGLQANHLSSWRSLARAGKLVLPAPEGSVEFAAMIVAPPGECPRRSEAVGPEIMV
GAVTVRLEAGAPVERIATLVRALSGPA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	466	819	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIFPSHRVRIMVATKPIDFRKGHDSLAALVKNELRKDPFTGTVFVFRARKADRLKLLYWDGTGLVMAYKRLEEHSFSWPPVKDGLMTLSHAQFEALFAGL
DWRRVRAVEARAPEAVE

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1539 bp	512 aa	888	2426	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLAPADLPDDIAALKAMLIASEARNNRKDERIERLEKLVAAFRQAAFGRRSEKSDPGQFELALEDLETAIAAIRAEEDAEDRAAKRPFRPRAINRGSLPR
HLPRIDEVIEPESLTCACGGCLHCIGEDVSERLDIVPAQFRVIVTRRPKYACRSCTDGVVQAPAPARLIPGGMPTEATVAHVLVSKYADHLPLYRQAQIY
SRQGIDLDRSTLADWVGRAAFELRPVFDALMADLKRSTKLSMDETRAPVLDPGARKTKTGYFWALARDDRPWGGTAPPGVAFTYAPGRGGQHAERILQGF
GGILQVDGYAGYNRLIAPDRVGPGIQLAYCWAHARRKLIEITRTGPAPIAEEGVDLIRDLYRIEADIRGSDPTARLVARQDRSAPILARLDDWLCHHRAR
ASAKSPLGEALAYIARYRDGLGRFLTDGRIEIDSNAVERTIRPIALNRKNALFAGHDTGAENWAVIASLIETCKLNSVDPQTWLANTLTAIANGHKQSQI
NDLLPWNYAAKV

Blast result :

Comments

ISUnCu14 is 74% (ORFA) aa similar to IS71, 78% (ORFB) to ISXau4 and 71% (ORFc : the transposase) to ISApr6.

References

1] ISfinder annotation (2010)
2] Suenaga,H., Ohnuki,T. and Miyazaki,K. (2007) Environ. Microbiol. 9 (9), 2289-2297
3] Suenaga,H., Koyama,Y., Miyakoshi,M., Miyazaki,R., Yano,H., Sota,M., Ohtsubo,Y., Tsuda,M. and Miyazaki,K. (2009) ISME J 3 (12), 1335-1348.