ISCsa2
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009437 | ND | Caldicellulosiruptor saccharolyticus | Caldicellulosiruptor saccharolyticus DSM 8903 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 14/23
IRL : GGGAGTTAAAATAAAATTGTGTCCACTGTATAATTAGTGTTGAAATAATC
IRR : GAGACTTGTCAAGAATTTTGTGTTTCCATTTCATAGCGTATATTGTAGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGAATGACA | GGTAGCGTG | TTTCATAACC | 9 |
| GTGAATCACT | CAAACTATT | TCTCCTGCTT | 9 |
| TTCACGAATA | CCTTGTTG | CAGTCCAAGG | 8 |
| ATAGCTCTCA | TCTTCATA | CTCAGGTAAA | 8 |
| TTCAAGCTTA | TTTACCTT | AACAGCTAAA | 8 |
| TACCCTCAAA | GAACACCC | TCAAAAAACT | 8 |
| TCTCCATTCG | GCACCTCT | CTCAAAAGCT | 8 |
| GAGAAATCTT | CTTTGCAG | TCTCCACAAC | 8 |
| AGGCATAAAG | CCTATTGT | TATTAATGGC | 8 |
| ATCCATTGCA | TATATCCT | CACATTGATA | 8 |
| CTCTAAAATC | AAATGCTC | TGCCTACATG | 8 |
| GGCAGTTGGC | TTTATTTC | TGAGGTTGGA | 8 |
| CATTTGTGGT | GCTGTTGC | AGTAGGGGTT | 8 |
| CCACTTTTAT | GCCATTCT | CTCTCAGATA | 8 |
| ACTGAAATCA | ATTTAGTA | CCATCTTCAA | 8 |
| CTAAGTTGCT | GTTTCAAA | AAATCTCTCT | 8 |
| AGCCAACTGC | CTGAGCTTTT | 0 | |
| TATCCCAGTG | TCTCAACTTC | 0 | |
| TGGTTGCTCT | TCCTTATAAT | 0 | |
| AACTGAGCTT | TCTCCAAGTA | 0 | |
| TTGTTATAAT | TCAACAATAA | 0 | |
| CAGAAATAAA | CATGTTGTCT | 0 | |
| TATCTCCAAG | TCCGCTCTTG | 0 | |
| ATAGCTCTCA | TGCTATGTAG | 0 | |
| GAATGCTATG | TAGCTCTCAT | 0 | |
| GAGTGGCAAA | TATCTTATAT | 0 | |
| TGAACAAGCT | GCCAGTAATG | 0 |
DNA sequence
GGGAGTTAAAATAAAATTGTGTCCACTGTATAATTAGTGTTGAAATAATCACCAAAGGGGGGCTCACGATTATGGAGAAAAATGAAATTTTTGAAACCGC
TAAAAATATGGCTATCGAGCAAGTATTAAATATGTATTGCTCCAAAGATGATCCTACTCGCCCAGCTTTAAAACAGCTTTTGGAAAACTTGCTCGATTGC
TTTATGTTATCAGAAAGAACTGTTTACCTTGCTAAAAACGAAAACGATAAAGGCAATGGTTTTTACGGCAGAAAACTTGCAACACCTGTTGGCAGCCTTG
AAATTTCTGTTCCTCGCACACGCTCTGGTAACTTTCGACCTTCCATTCTCCCTGACCGCTACAAAAGGGTTGACAGCTCATACACTGACCTGCTCATGTC
TTTAGTCGCCAATGGTTACTCAGAAAGTTCTCTTGTCCAAACTCTTAAAAGCATGAATCTGCCTTATTCTGAAGACGAAATCGAAAAAATCAAAAACGAT
CTTAAAAACGAGCTTCAACTTTTCAAACAAAGAGAACTTCCTGAAAGTGCTTTTGCTCTTATCATTGACGGTTACCATTGCGAAATTAAAGATAACTCAA
AAGTTAAACAAGCTACTTGCTATGTCGTGCTTGGCATTGATTTAGAAGGCAAAAAAGATATCTTCGGTATCTACACTTTCTTCGGCAAAGAAAACAAAGC
CGATTGGATGAGAGTCTTTGACGACTTAATTACAAGAGGTCTTAAAAAAGTCTTAATAGTTGTAAGCGATGATTTTCCAGGCATTATCGATGCTGTTAGA
CTCGCTTATCCCCTTGCCGACCATCAACTATGTTTTGTTCACCTTCAACGCAATGTCAGAAAACATATGGCAAAAGATGATGCTTCCGTTTTCAACAAAG
AGCTTGATAAACTAAGAACTTCCTCTGCTGATTTTGACGAAGCTATTTCAAAGTTCAAACTTCTTTGTGAGCAATACTCCTCAAAATATCCTCGATTCAT
AAAAGGTATTTGCGAAAAAGCAGAGTTCTATCTTGCACATATGAGGTATCCTGAAGATTTAAGAAAGTACATTTATACTACTAATGCTGTAGAAAGCGTA
AACAGTATGATTGAAAAGATAAGAATAAACTCCGGTGGTTATTTTCAATCTGTAGAAGTTTTAGAGATAAACATATATTTACAAAGAGAAAACTTGCGTC
GGGGCAAGTGGAAAAACGGAGTACCTATTCTTAAAAAATGTAGTTACAATATATTACAGCTCTACAATATACGCTATGAAATGGAAACACAAAATTCTTG
ACAAGTCTC
TAAAAATATGGCTATCGAGCAAGTATTAAATATGTATTGCTCCAAAGATGATCCTACTCGCCCAGCTTTAAAACAGCTTTTGGAAAACTTGCTCGATTGC
TTTATGTTATCAGAAAGAACTGTTTACCTTGCTAAAAACGAAAACGATAAAGGCAATGGTTTTTACGGCAGAAAACTTGCAACACCTGTTGGCAGCCTTG
AAATTTCTGTTCCTCGCACACGCTCTGGTAACTTTCGACCTTCCATTCTCCCTGACCGCTACAAAAGGGTTGACAGCTCATACACTGACCTGCTCATGTC
TTTAGTCGCCAATGGTTACTCAGAAAGTTCTCTTGTCCAAACTCTTAAAAGCATGAATCTGCCTTATTCTGAAGACGAAATCGAAAAAATCAAAAACGAT
CTTAAAAACGAGCTTCAACTTTTCAAACAAAGAGAACTTCCTGAAAGTGCTTTTGCTCTTATCATTGACGGTTACCATTGCGAAATTAAAGATAACTCAA
AAGTTAAACAAGCTACTTGCTATGTCGTGCTTGGCATTGATTTAGAAGGCAAAAAAGATATCTTCGGTATCTACACTTTCTTCGGCAAAGAAAACAAAGC
CGATTGGATGAGAGTCTTTGACGACTTAATTACAAGAGGTCTTAAAAAAGTCTTAATAGTTGTAAGCGATGATTTTCCAGGCATTATCGATGCTGTTAGA
CTCGCTTATCCCCTTGCCGACCATCAACTATGTTTTGTTCACCTTCAACGCAATGTCAGAAAACATATGGCAAAAGATGATGCTTCCGTTTTCAACAAAG
AGCTTGATAAACTAAGAACTTCCTCTGCTGATTTTGACGAAGCTATTTCAAAGTTCAAACTTCTTTGTGAGCAATACTCCTCAAAATATCCTCGATTCAT
AAAAGGTATTTGCGAAAAAGCAGAGTTCTATCTTGCACATATGAGGTATCCTGAAGATTTAAGAAAGTACATTTATACTACTAATGCTGTAGAAAGCGTA
AACAGTATGATTGAAAAGATAAGAATAAACTCCGGTGGTTATTTTCAATCTGTAGAAGTTTTAGAGATAAACATATATTTACAAAGAGAAAACTTGCGTC
GGGGCAAGTGGAAAAACGGAGTACCTATTCTTAAAAAATGTAGTTACAATATATTACAGCTCTACAATATACGCTATGAAATGGAAACACAAAATTCTTG
ACAAGTCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1230 bp | 409 aa | 72 | 1301 | + | No |
Chemistry : DDE
ORF sequence :
MEKNEIFETAKNMAIEQVLNMYCSKDDPTRPALKQLLENLLDCFMLSERTVYLAKNENDKGNGFYGRKLATPVGSLEISVPRTRSGNFRPSILPDRYKRV
DSSYTDLLMSLVANGYSESSLVQTLKSMNLPYSEDEIEKIKNDLKNELQLFKQRELPESAFALIIDGYHCEIKDNSKVKQATCYVVLGIDLEGKKDIFGI
YTFFGKENKADWMRVFDDLITRGLKKVLIVVSDDFPGIIDAVRLAYPLADHQLCFVHLQRNVRKHMAKDDASVFNKELDKLRTSSADFDEAISKFKLLCE
QYSSKYPRFIKGICEKAEFYLAHMRYPEDLRKYIYTTNAVESVNSMIEKIRINSGGYFQSVEVLEINIYLQRENLRRGKWKNGVPILKKCSYNILQLYNI
RYEMETQNS
DSSYTDLLMSLVANGYSESSLVQTLKSMNLPYSEDEIEKIKNDLKNELQLFKQRELPESAFALIIDGYHCEIKDNSKVKQATCYVVLGIDLEGKKDIFGI
YTFFGKENKADWMRVFDDLITRGLKKVLIVVSDDFPGIIDAVRLAYPLADHQLCFVHLQRNVRKHMAKDDASVFNKELDKLRTSSADFDEAISKFKLLCE
QYSSKYPRFIKGICEKAEFYLAHMRYPEDLRKYIYTTNAVESVNSMIEKIRINSGGYFQSVEVLEINIYLQRENLRRGKWKNGVPILKKCSYNILQLYNI
RYEMETQNS
Blast result :
Comments
ISCsa2 is 53% aa similar to ISC1232.
References
1] Land M. (2010) Direct submission.
2] van de Werken,H.J., Verhaart,M.R., VanFossen,A.L., Willquist,K., Lewis,D.L., Nichols,J.D., Goorissen,H.P., Mongodin,E.F., Nelson,K.E., van Niel,E.W., Stams,A.J., Ward,D.E., de Vos,W.M., van der Oost,J., Kelly,R.M. and Kengen,S.W. (2008) Appl. Environ. Microbiol. 74 (21), 6720-6729.
3] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P. (2007) Direct submission GenBank.
2] van de Werken,H.J., Verhaart,M.R., VanFossen,A.L., Willquist,K., Lewis,D.L., Nichols,J.D., Goorissen,H.P., Mongodin,E.F., Nelson,K.E., van Niel,E.W., Stams,A.J., Ward,D.E., de Vos,W.M., van der Oost,J., Kelly,R.M. and Kengen,S.W. (2008) Appl. Environ. Microbiol. 74 (21), 6720-6729.
3] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P. (2007) Direct submission GenBank.