ISfinder Mirror Site

ISStsp5

Family IS5
Group ISL2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_ADFD01000026	ND	Streptomyces sp.	Streptomyces sp. ACTE

DNA section

IS Length : 885 bp

Ends

IR Length : 20/22

IRL : GAGCGTTTTCAGCGTGGCCACTGATCGAGTGACGCCGGTCAGGTTGAGGT
IRR : GAGCGTTTTCAGCGTTGCCGCTCCAGGGCGAGGACGGCTTTGGTGATGAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTGTCAGACG		ACTGTTGCGC	0

DNA sequence

GAGCGTTTTCAGCGTGGCCACTGATCGAGTGACGCCGGTCAGGTTGAGGTGAGGGCCGCAACGCACAAAGCCCCCGTGCCGTTGAGGGAGGTGTTCGACG
TCTCAACCCACCGGCACAGGAGCCCTGTTGGTTCCCTATCCTGCCGCACTCGACCTTCCTCACGCCCTGGTCGAGTGGGTCACCATGCTCATCGTCACCC
GCGAGGGTGACCGCCGCTGCAAGCTCCCGCCGCACCAGCGTGCGCTCGTCGCTCTGGTGTACCTGCGCCGCCACGACACCCTCGCCCGGATCGCGACCGG
CTTCGGGATATCCGTCGGCACCGCCCACGCCTACGTCACCGCCGTGACCGGCCTGCTCGCCGAACGAGCCCCCGGCCTGCTCAGAACGCTGCGCGAGCAG
GATCCGGAGTTCGTCCTGCTGGACGGGACCCTCGCCGAATGCGACCGCGTCGGCGACAGCCGGGCCGACTACTCGGCCAAGCACCGCCGGCACGGCGTGA
ACATCCAGGTCGTGACCAATCCGACGGGCGAACTGCTGTGGATCTCGCCCGCGCTGCCCGGTCGCACCCATGACCTGACCGCGGCCCGAACCCATCGCAT
CATCCGGATCTGCGAACGCCAGGGCATCCCCGTCCTCGCCGACCGTGCCTACCAGGGTGCCGGTTTCTGGGTCACCACTGGACTCAAACGGCCACCGGGT
GGCGAGCTCACCCTCACCCAGCGCACCGTCAACCGGGCCCTGGCCGCAGCCCGAGCACCGGTCGAACGCGGCATGGCACGGCTGAAGTCCTGGCAGATCT
TCCGCAGATCCCGCATCAGCCCGAACCGCATGACCGTCATCACCAAAGCCGTCCTCGCCCTGGAGCGGCAACGCTGAAAACGCTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
750 bp	249 aa	128	877	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVPYPAALDLPHALVEWVTMLIVTREGDRRCKLPPHQRALVALVYLRRHDTLARIATGFGISVGTAHAYVTAVTGLLAERAPGLLRTLREQDPEFVLLDG
TLAECDRVGDSRADYSAKHRRHGVNIQVVTNPTGELLWISPALPGRTHDLTAARTHRIIRICERQGIPVLADRAYQGAGFWVTTGLKRPPGGELTLTQRT
VNRALAAARAPVERGMARLKSWQIFRRSRISPNRMTVITKAVLALERQR

Blast result :

Comments

ISStsp5 is 57% aa similar to ISSro1.

References

1] ISfinder annotation (2010).
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L., Adams,A., Raffa,K., Adams,S. and Currie,C. (2009) Direct submission GenBank.