ISfinder Mirror Site

ISHla4

Family IS5
Group ISH1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012028	ND	Halorubrum lacusprofundi	Halorubrum lacusprofundi ATCC 49239 chromosome 2

DNA section

IS Length : 1804 bp

Ends

IR Length : 17

IRL : GGCTCTGTTGAAATCCTCAGAGATGTACATGTTCGCCGTGTAATTCTATG
IRR : GGCTCTGTTGAAATCCTATTCATCAGACATATACACAGATGAAATAGCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATACGAAGAT		GGACTACCTT	0

DNA sequence

GGCTCTGTTGAAATCCTCAGAGATGTACATGTTCGCCGTGTAATTCTATGGGATGAAGTCCCTCCCAAAGTCGCAGATTCTCCGGTTTACTGAGAAGGCG
ATCCATCTGGCACGCCGGGCGGTCTCTCGGTACTCCTCGAAATTCTCTAAACACCGCTATACACTTCCGCAGCACGTTGTTCTGCTCTGTCTCAAAGTTC
GGAAGAACACGACCTACCGTGGTCTGCTTGACGAACTGATCGAGATGCCACGCATCCGTCGTGTTCTCGGGCTAGCCGAACTTCCTACTGCTTCAACGCT
TTGTAAGTCGTTCAGCCGGCTTGATATGGCTGTATGGCGTGTCATATTGACTCTCTCAGCGACACTACTTCCGACAAGCGGCGTTGTTGGTGTTGATGCG
TCAGGGTTCGACCGCAGTCACGCTTCGAAACACTACACGAAACGCGCTGAACTCACGATTCAGCAGCTCAAGGTGACGTTACTGGTCGATGCGAAGGTAA
ACGCGATACTCGATCTACACGTAACTACGACGCGGAAACACGATAGCCAGATCGCTCCGTCGTTGATCAAGCGCAATCCCGACGATATTGACGTTTTGCT
CGGTGACAAAGGGTACGACGATCAGAAGATCAGGCGGCTCGCCCGGCAACACGAAGTTCGACCACTGATCAAGCATCGTGAGTTCACGTCACTCCATAAG
GCATGGAACGTACGCTTAGACACTGATCTCTACGGTCAGCGGAGTCAATCCGAGACTGTCAACTCAACACTCAAGCGGAAGTACGGGGCGTTTGTCCGGT
CACGGCGCTGGTGGAAGCAGTTCCGTGAACTCACCATCGCCTGTCTCATTCATAACGTAGATCGATCACTCTGAGCGGTCAATACAGGAAACTCACGAAT
CGCTCAGTGCAGGAGACGCAGAGATCGCTCAGAGTGTTCTGTTTCGACCCACAAGACATACTCTTTGTCAGCAAAGCCGAATACGTATGTCCCCGACGAA
ACGTCTAATTGGCACCACTTTACGCGAAAAAATTACTGTGATCCTCTTGGCAGCGTCAATGCTAATCTCCGTGGTCGCAGTTGGCTTTGTTTCCGACCCC
GTCGCAGCCACGGCCGGTCATTCCCCAAGTTTTGGCGAAAGTGTTACTGATACAATCGCTGATACCAATGTACCAAATACAGCCAACGAGACCAACAAAA
GTGAGGTCTACTTTGAGATCGCTAACCTCACGCCTCAGGCTCGGGACACTACCGTGGCTCTTGACGACACATTAGCATTCACCGTTACGATCGAAAATAC
TGGTGATGCCAAAGGCGTTCAGACCGTTGAGTTTCGCATCGGCGACAACGCGGTCGCCAGCCGAAAAGTGACGCTTGATGTAAACAACAGCACCACTATT
GAATTTGATGAGATCAACGTGTCACACTTTGACACGGGTGAGTACGAGTACGGCTTCCTTACGAATGACGACAACCGGACTGTAACCATCACATTCCAGC
CTGCTGGTGACGACCGCGTTGTTGTCGATAGAGACAATGATACCGATAGTGAGAACGACTCAAACGGAAGTGACGGTAACGATGAACGGGACGGGGCTAC
CAGTAATGACACGCCCGGCTTCGGTGCGTCTGTGGCTCTCGTTGCTCTCGTCGTAGCTACGCTGTTCATGACCCGTCCCAGCGAGTAGGATCTTTGAATT
CCGGCTGAGTAGAGCGTACAAGGATGTTTTGAGCGATTCGCAGCTCTCACCAACTGGCTATTTCATCTGTGTATATGTCTGATGAATAGGATTTCAACAG
AGCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
822 bp	273 aa	53	874	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSLPKSQILRFTEKAIHLARRAVSRYSSKFSKHRYTLPQHVVLLCLKVRKNTTYRGLLDELIEMPRIRRVLGLAELPTASTLCKSFSRLDMAVWRVILT
LSATLLPTSGVVGVDASGFDRSHASKHYTKRAELTIQQLKVTLLVDAKVNAILDLHVTTTRKHDSQIAPSLIKRNPDDIDVLLGDKGYDDQKIRRLARQH
EVRPLIKHREFTSLHKAWNVRLDTDLYGQRSQSETVNSTLKRKYGAFVRSRRWWKQFRELTIACLIHNVDRSL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
702 bp	233 aa	987	1688	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSPTKRLIGTTLREKITVILLAASMLISVVAVGFVSDPVAATAGHSPSFGESVTDTIADTNVPNTANETNKSEVYFEIANLTPQARDTTVALDDTLAFTV
TIENTGDAKGVQTVEFRIGDNAVASRKVTLDVNNSTTIEFDEINVSHFDTGEYEYGFLTNDDNRTVTITFQPAGDDRVVVDRDNDTDSENDSNGSDGNDE
RDGATSNDTPGFGASVALVALVVATLFMTRPSE

Blast result :

Comments

ISHla4 (orfA, the transposase) is 96% aa similar to ISHma13. The orfB is a passenger gene.

References

1] ISfinder annotation (2010).

2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.