ISBdo1
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_CH482381 | ND | Burkholderia dolosa | Burkholderia dolosa AUO158 |
DNA section
IS Length : 1572 bp
Ends
IR Length : 19
IRL : CAGACTGCTGACAAACCCCTGGTTTCGACCGGGGGTTTGTCGTTTAATGG
IRR : CAGACTGCTGACAAACCCCATGTTTTTCGGAACATGGGGTTTTGTCGTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAAGCAAAA | ACGCCACCCGAAGGTGGCGTT | TCGTTTTTGC | 21 |
DNA sequence
CAGACTGCTGACAAACCCCTGGTTTCGACCGGGGGTTTGTCGTTTAATGGGGCATGCTCAAGAAGCCAACACCCGCCCAAGAAGAACTCGAGATGGTGAC
GCTCGAGATGCTTGTGCCGAAGGACCATCTGCTGCGCAAGATCGAGGCGGCGGTGGATTTCGAATTTATCCGCGAGAAGGTCGCGCATCTGTACTGCGCG
GACAACGGTCGCCCGGCGCTCGATCCGGTCGTGCTGTTCAAGCTGTTGTTGATCGGCTACTTGTTCGGGGTGCGCAGCGAGCGGCAGCTCATGCGCGAAG
TGCAGGTCAATGTGGCGTATCGGTGGTTTGCCGGGTTTCGTCTGACCGATAAAGTGCCGGATGCCTCGACGTTCTCGCAGAACCGTCGGCGGCGCTTCAC
CGACACGACGGTGTATCAAGAGATCTTCGATGAGATCGTGCGCCAGGCAATCGGGCGCGGCATGGTCGATGGCCGAGTGCTGTACAGCGACAGCACGCAT
CTGAAGGCCAACGCCAACAAGAACAAGTTCGACGAAGTGCAGGTTGCGCAAACGCCGTCGGCATATCTGGCCGAGTTGAATGCGGCGATCGATGCGGACC
GTGCCGCACAAGGCAAGAAGCCTTTGAAGCGCGATGACGACGACCAACCGCCGCCGAGCAAACAGATCAAGGTTAGCCGCACCGATGCCGAGAGCGGCTA
CATGGTGCGCGACGACAAGCCCAAGGGCTTCTTTTATCTGGATCACCGCACGGTAGACGCCAAGCACGCGATCATCACCGATACCCACGTGACGCCGGCC
TCGGTGCACGACAGTCAGCCATATCTGGCGCGCTTAGACCGCCAACGCAAACGCTTCGGGTTTGAGGTGCAGGCGGTGGGACTTGATGCGGGCTACTTCA
CGCCCGCGATCTGCCAGGGGCTGGAGGATCGCGGTGTCACTGGCGTGATGGGCTATCGCTCGCCGAACCACAAGCCGGGTACGTTCTATAAGCGCGAGTA
CCGCTACGACGCCTATCGGGACGAGTACATGTGTCCGCAGGGCCAGACGCTGCGCTACAGCACCACCAACCGGGTGGGCTACCGCGAATACAAGTCCAAC
CCTGAGCTGTGCAAGCGCTGCGAGGCCCGTAGTCAATGCACGAGCAGTGCCAATGCGATCAAGGTGGTAGTGCGTCACGTTTGGGAGCGTGCCAAGGAGC
GCGTGGACGCTCGGCGGCTGACCGAATGGGGCAAACGCATTTATGCCAGGCGCAAGGAAACCGTGGAGCGCAGCTTCGCGGACGCCAAGCAGCTGCACGG
GCATCGCTACGCCAGAATGCGCGGGCTGCGCAAAGTGGCCGAACAGTGCTTGCTGGCGGCCGCGGCACAGAACATCAAGAAGATCGCGCTGCTGCTGACG
CGCCTGCGGGCGCTTTTACCCGGCTTCTGGCGCTCTCGTGCGCCGTTGCAGCGGCTCATCGCCGATCTCACCGGCTTGCTTGACCCGGTCGCGGCTTGCC
GCATCAACAGCGCAGCCGCCTAAAACGACAAAACCCCATGTTCCGAAAAACATGGGGTTTGTCAGCAGTCTG
GCTCGAGATGCTTGTGCCGAAGGACCATCTGCTGCGCAAGATCGAGGCGGCGGTGGATTTCGAATTTATCCGCGAGAAGGTCGCGCATCTGTACTGCGCG
GACAACGGTCGCCCGGCGCTCGATCCGGTCGTGCTGTTCAAGCTGTTGTTGATCGGCTACTTGTTCGGGGTGCGCAGCGAGCGGCAGCTCATGCGCGAAG
TGCAGGTCAATGTGGCGTATCGGTGGTTTGCCGGGTTTCGTCTGACCGATAAAGTGCCGGATGCCTCGACGTTCTCGCAGAACCGTCGGCGGCGCTTCAC
CGACACGACGGTGTATCAAGAGATCTTCGATGAGATCGTGCGCCAGGCAATCGGGCGCGGCATGGTCGATGGCCGAGTGCTGTACAGCGACAGCACGCAT
CTGAAGGCCAACGCCAACAAGAACAAGTTCGACGAAGTGCAGGTTGCGCAAACGCCGTCGGCATATCTGGCCGAGTTGAATGCGGCGATCGATGCGGACC
GTGCCGCACAAGGCAAGAAGCCTTTGAAGCGCGATGACGACGACCAACCGCCGCCGAGCAAACAGATCAAGGTTAGCCGCACCGATGCCGAGAGCGGCTA
CATGGTGCGCGACGACAAGCCCAAGGGCTTCTTTTATCTGGATCACCGCACGGTAGACGCCAAGCACGCGATCATCACCGATACCCACGTGACGCCGGCC
TCGGTGCACGACAGTCAGCCATATCTGGCGCGCTTAGACCGCCAACGCAAACGCTTCGGGTTTGAGGTGCAGGCGGTGGGACTTGATGCGGGCTACTTCA
CGCCCGCGATCTGCCAGGGGCTGGAGGATCGCGGTGTCACTGGCGTGATGGGCTATCGCTCGCCGAACCACAAGCCGGGTACGTTCTATAAGCGCGAGTA
CCGCTACGACGCCTATCGGGACGAGTACATGTGTCCGCAGGGCCAGACGCTGCGCTACAGCACCACCAACCGGGTGGGCTACCGCGAATACAAGTCCAAC
CCTGAGCTGTGCAAGCGCTGCGAGGCCCGTAGTCAATGCACGAGCAGTGCCAATGCGATCAAGGTGGTAGTGCGTCACGTTTGGGAGCGTGCCAAGGAGC
GCGTGGACGCTCGGCGGCTGACCGAATGGGGCAAACGCATTTATGCCAGGCGCAAGGAAACCGTGGAGCGCAGCTTCGCGGACGCCAAGCAGCTGCACGG
GCATCGCTACGCCAGAATGCGCGGGCTGCGCAAAGTGGCCGAACAGTGCTTGCTGGCGGCCGCGGCACAGAACATCAAGAAGATCGCGCTGCTGCTGACG
CGCCTGCGGGCGCTTTTACCCGGCTTCTGGCGCTCTCGTGCGCCGTTGCAGCGGCTCATCGCCGATCTCACCGGCTTGCTTGACCCGGTCGCGGCTTGCC
GCATCAACAGCGCAGCCGCCTAAAACGACAAAACCCCATGTTCCGAAAAACATGGGGTTTGTCAGCAGTCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1470 bp | 489 aa | 54 | 1523 | + | No |
Chemistry : DDE
ORF sequence :
MLKKPTPAQEELEMVTLEMLVPKDHLLRKIEAAVDFEFIREKVAHLYCADNGRPALDPVVLFKLLLIGYLFGVRSERQLMREVQVNVAYRWFAGFRLTDK
VPDASTFSQNRRRRFTDTTVYQEIFDEIVRQAIGRGMVDGRVLYSDSTHLKANANKNKFDEVQVAQTPSAYLAELNAAIDADRAAQGKKPLKRDDDDQPP
PSKQIKVSRTDAESGYMVRDDKPKGFFYLDHRTVDAKHAIITDTHVTPASVHDSQPYLARLDRQRKRFGFEVQAVGLDAGYFTPAICQGLEDRGVTGVMG
YRSPNHKPGTFYKREYRYDAYRDEYMCPQGQTLRYSTTNRVGYREYKSNPELCKRCEARSQCTSSANAIKVVVRHVWERAKERVDARRLTEWGKRIYARR
KETVERSFADAKQLHGHRYARMRGLRKVAEQCLLAAAAQNIKKIALLLTRLRALLPGFWRSRAPLQRLIADLTGLLDPVAACRINSAAA
VPDASTFSQNRRRRFTDTTVYQEIFDEIVRQAIGRGMVDGRVLYSDSTHLKANANKNKFDEVQVAQTPSAYLAELNAAIDADRAAQGKKPLKRDDDDQPP
PSKQIKVSRTDAESGYMVRDDKPKGFFYLDHRTVDAKHAIITDTHVTPASVHDSQPYLARLDRQRKRFGFEVQAVGLDAGYFTPAICQGLEDRGVTGVMG
YRSPNHKPGTFYKREYRYDAYRDEYMCPQGQTLRYSTTNRVGYREYKSNPELCKRCEARSQCTSSANAIKVVVRHVWERAKERVDARRLTEWGKRIYARR
KETVERSFADAKQLHGHRYARMRGLRKVAEQCLLAAAAQNIKKIALLLTRLRALLPGFWRSRAPLQRLIADLTGLLDPVAACRINSAAA
Blast result :
Comments
ISBdo1 is 88% aa similar to ISBma2.
References
1] ISfinder annotation (2010)
2] Birren,B., Lander,E., Galagan,J., Devon,K., Nusbaum,C., Borowsky,M.L., Jaffe,D., Butler,J., Alvarez,P., Gnerre,S., Grabherr,M., Kleber,M., Mauceli,E., Brockman,W., Young,S., LaButti,K., DeCaprio,D., Crawford,M., Koehrsen,M., Engels,R., Montgomery,P., Pearson,M., Howarth,C., White,J., Larson,L., Alvarado,L., Kodira,C., Zeng,Q., Yandava,C., Oleary,S., LiPuma,J. and Lory,S. (2005) Direct submission GenBank.
2] Birren,B., Lander,E., Galagan,J., Devon,K., Nusbaum,C., Borowsky,M.L., Jaffe,D., Butler,J., Alvarez,P., Gnerre,S., Grabherr,M., Kleber,M., Mauceli,E., Brockman,W., Young,S., LaButti,K., DeCaprio,D., Crawford,M., Koehrsen,M., Engels,R., Montgomery,P., Pearson,M., Howarth,C., White,J., Larson,L., Alvarado,L., Kodira,C., Zeng,Q., Yandava,C., Oleary,S., LiPuma,J. and Lory,S. (2005) Direct submission GenBank.