ISCaa4
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010830 | ND | Candidatus Amoebophilus | Candidatus Amoebophilus asiaticus 5a2 |
DNA section
IS Length : 732 bp
Ends
IR Length : 20/22
IRL : GGTATTGTTAAATTGGGATAGGATTAGTAAGTTAGAGGAAAAAGTATGAA
IRR : GGTATTGTTAAATTAAGATAGGCAGCTGATTATTTAGCTTGGCCATTAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTCCTATTA | GTATTTTT | AATGTAATAC | 8 |
| TTAGGCATAT | AAGTAATA | AATTCTAGTT | 8 |
| TGATATATAA | AAAATAAG | AATAGGTAAG | 8 |
| TGGATAACTA | ACTTTTTC | AGATAAAAAT | 8 |
| AAAAGCTATT | AAAAAATT | CTATAAAGAT | 8 |
| TCCCTTTAAA | AAAATAAC | ATATATTGTT | 8 |
| CATATTAATA | AATATTTA | GATCATATGC | 8 |
| AACGGGATAG | AAAATTAA | TAATCTTTGG | 8 |
| TGCCTTATTA | AAATAGGC | TTCTTGCATA | 8 |
| TTTATAAATA | ATAAATTT | AACTCTCAAA | 8 |
| TCTTCAACAC | AAATTTTt/g | TGCTATTACA | 8 |
| ACCAGGACTG | AAAATt/aT | AACAATTAAT | 7 |
DNA sequence
GGTATTGTTAAATTGGGATAGGATTAGTAAGTTAGAGGAAAAAGTATGAATTGTCCTCGATGTAATAATACTCAAAGCTGTAAAGATGGAATTGTTAGAG
GTAGACAGCGCTACCAGTGTAAAAGTTGCCGTTTCCCTTACACAGTTAGTCACAAATCAGATGTTAAACCTGTATCTACTAAGCGAAAAGCGTTGCAATT
ATACTTAGAAGGATTAGGATTTCGAGCTATAGGCCGTATACTCAACATAAGCTATGGAACAGTCTATCAATGGGTAAAAGCATGTGGAGATCAAGTAAGT
TTACCAGAAAGACAAGATCAAGTAGATATAGTCGAGATGGATGAAATACACACATATGTGGGTTCAAAAAAGTCTACTGCTGGATATGGATAGCTGTTGA
TAGATTGAGCAAGCGTTTTATATCATATGTGTGTGGAGATCGCTCGACACAAACCGGATTGAAGTTATGGGAGCGCGTCAAGGATATAGGCAAGCTGTAT
TGTAGTGATTATTGGAAAAGCTACCAGCAGTTTATTCCAAAAGATAAACACCGACAAAGCAAATCAGAAACTTATACAGTGGAAGGATATAATAGCTTAA
TGAGGCACTATTTAGCAAGATTTAAGCGTAAAGGAAAATGCTATAGCAAACAGGTGCACATGATAGAAAAATCGCTCAACCTGCTAATGGCCAAGCTAAA
TAATCAGCTGCCTATCTTAATTTAACAATACC
GTAGACAGCGCTACCAGTGTAAAAGTTGCCGTTTCCCTTACACAGTTAGTCACAAATCAGATGTTAAACCTGTATCTACTAAGCGAAAAGCGTTGCAATT
ATACTTAGAAGGATTAGGATTTCGAGCTATAGGCCGTATACTCAACATAAGCTATGGAACAGTCTATCAATGGGTAAAAGCATGTGGAGATCAAGTAAGT
TTACCAGAAAGACAAGATCAAGTAGATATAGTCGAGATGGATGAAATACACACATATGTGGGTTCAAAAAAGTCTACTGCTGGATATGGATAGCTGTTGA
TAGATTGAGCAAGCGTTTTATATCATATGTGTGTGGAGATCGCTCGACACAAACCGGATTGAAGTTATGGGAGCGCGTCAAGGATATAGGCAAGCTGTAT
TGTAGTGATTATTGGAAAAGCTACCAGCAGTTTATTCCAAAAGATAAACACCGACAAAGCAAATCAGAAACTTATACAGTGGAAGGATATAATAGCTTAA
TGAGGCACTATTTAGCAAGATTTAAGCGTAAAGGAAAATGCTATAGCAAACAGGTGCACATGATAGAAAAATCGCTCAACCTGCTAATGGCCAAGCTAAA
TAATCAGCTGCCTATCTTAATTTAACAATACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 46 | 393 | + | No |
Description : First part of the transposase
ORF sequence :
MNCPRCNNTQSCKDGIVRGRQRYQCKSCRFPYTVSHKSDVKPVSTKRKALQLYLEGLGFRAIGRILNISYGTVYQWVKACGDQVSLPERQDQVDIVEMDE
IHTYVGSKKSTAGYG
IHTYVGSKKSTAGYG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 381 bp | 126 aa | 345 | 725 | + | No |
Description : Second part of the transposase
ORF sequence :
NTHICGFKKVYCWIWIAVDRLSKRFISYVCGDRSTQTGLKLWERVKDIGKLYCSDYWKSYQQFIPKDKHRQSKSETYTVEGYNSLMRHYLARFKRKGKCY
SKQVHMIEKSLNLLMAKLNNQLPILI
SKQVHMIEKSLNLLMAKLNNQLPILI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 680 bp | 226 aa | 46 | 725 | + | Yes |
Chemistry : DDE
ORF sequence :
MNCPRCNNTQSCKDGIVRGRQRYQCKSCRFPYTVSHKSDVKPVSTKRKALQLYLEGLGFRAIGRILNISYGTVYQWVKACGDQVSLPERQDQVDIVEMDE
IHTYVGSKKVYCWIWIAVDRLSKRFISYVCGDRSTQTGLKLWERVKDIGKLYCSDYWKSYQQFIPKDKHRQSKSETYTVEGYNSLMRHYLARFKRKGKCY
SKQVHMIEKSLNLLMAKLNNQLPILI
IHTYVGSKKVYCWIWIAVDRLSKRFISYVCGDRSTQTGLKLWERVKDIGKLYCSDYWKSYQQFIPKDKHRQSKSETYTVEGYNSLMRHYLARFKRKGKCY
SKQVHMIEKSLNLLMAKLNNQLPILI
Blast result :
Comments
ISCaa4 is 73% aa similar to ISMac16.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISCaa4 is present in 24 copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISCaa4 is present in 24 copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
References
1] Schmitz-Esser S, Tischler P, Arnold R, Montanaro J, Wagner M, Rattei T, Horn M. (2010) J Bacteriol., 192(4):1045-1057.