ISMae28
- Family ISAzo13
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010296 | ND | Microcystis aeruginosa | Microcystis aeruginosa NIES-843 |
DNA section
IS Length : 1595 bp
Ends
IR Length : 19/22
IRL : GGGACTTCCAAAAAATAAACTATTCATGCTCAAGAATAATAATCATTCTC
IRR : GGGACTTCCAGAGAATAAAATACCCAACAAAAAAATAGAAAAGTGTTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGCGGCA | TTA | CCTGGTTT | 3 |
| TTTAGGAA | TAA | CTATTCCC | 3 |
| AAACCCTA | TTA | GGGATTGA | 3 |
| GGGGGTAG | TTA | TGCCATTG | 3 |
| CCACTTAT | TAA | CCGTAACT | 3 |
| CATCATAGTAA | TAGAGGGGAAC | 0 | |
| CCTCAGACCTA | TTAAAGCATTA | 0 | |
| ATGTGTAA | TTA | ATTTTGCT | 3 |
DNA sequence
GGGACTTCCAAAAAATAAACTATTCATGCTCAAGAATAATAATCATTCTCGGAGTGTAGGAGGTAGGGGTCGATGGTTGCCTCCTCCTTCCAATTTTGTA
GTAGAATTAATCCTGTACATTATTAATCAAGTTTTTCTTTGTAAGTGTATATGGAATTAACTGATTCCCTCAAGAAATTGCTCAGTGAAACTGCACTTCA
ATTAAAAGGTGCAGCTAAAAGAAGATTCATGGCCCAAACAGTCTTAGAATTAGGCTATGGGGGACAAACCCTTGCTGCACAGGAGTTAGGCTGGAATCGA
ACTACTATTCGTAAAGGAATTAAAGAACTAAAAAGAGGTATTATTTGTGTTGATAATCATTCAGCTAAGGGGCGGAAAAAAGCAGAAGAACATTTACCTT
TTCTATTGGAAAACATCAAAAGTTTAGTGGATTCTCAAAGCCAAACTGACCCAAGTTTTAAAAGCCAAAGGCTTTATGTGAGACTGAGTGCGGCCGAAGT
CCGAAAGCAATTAATCTCTAAATATGGGTACAGTGATGAGGATTTACCGAGCGAGGAAACTATTCGGGTTAAATTAAATAACTTAGGTTATCGTCTGAAA
AGAGTCGCTAAAGTTTTACCTCAAAAAAAATTCCAGAAACCGAGGCAATCTTTGAGGAATTAGCTAACATTAATCGGGAAGCGGATGAAGACCCTACGAT
GTTACGTCTTAGTTTGGATGCCAAAGCCCGTGTTAATATTGGACTATTTGATCGAGGAGGTAAGAATAGAATAACTGTCGAAACAAACGATCATGATTTT
AATCCGAAAACAACCCTAACCCCTTACGGAATATTTATTCCAGAATTTGATGAGTTGTTTTTGTATTTCACTGCCTCCACAGTCACCAGTGACTTTATTG
TTGATATATTAGAAGATTTCTGGGAGTCGGAAAAATCTCGTTTTGAGAAAATTAAAACTTTGATAATTAATCAAGATAATGGACCAGAAAATAATTCGAG
ACGAACTCAGTTCATGAAACGTATAGTTGAGTTTTCCCAAAAATATCAAGTTAATATACGTTTAGCTTACTATCCCCCTTACCATAGTAAATATAATCCT
ATTGAACGAACCTGGGCTGTGTTAGAAAACCCTTGGAATGGGAGTATTTTAGATGAAATCGAAACGGCTTTGAAATTCGCCCAAACTATGACTTGGAAAG
GAAAACACCCGATTGTTAAGTTGATTACTGAAACTTATGAAAAAGGAGTAAAGCTTACTAAAAAAGCCATGGAAAAAATCGAAGAAAAAATCGAACGTCT
CACAGAATCAACGAATCAAGACTTTCCCGATTTGGGACAATGGTTTATTGATATCTATTATGATAAGACCTAGTTTGTAGTTAACTAAGATGGTTAGCTA
AAAACTGTCTTTGATTCTTTAACTTGTCCTTGTTATACTCGAAAAAGCTCGGAAGTATAAGAGTTTGTGCTGCCTATTTTTTCGGAAAAATAGGTTGTAA
TGGGAGACTCTTTGTTTTCCTAGACAAAAATAATTACTTTTTCAAAAAAACACTTTTCTATTTTTTTGTTGGGTATTTTATTCTCTGGAAGTCCC
GTAGAATTAATCCTGTACATTATTAATCAAGTTTTTCTTTGTAAGTGTATATGGAATTAACTGATTCCCTCAAGAAATTGCTCAGTGAAACTGCACTTCA
ATTAAAAGGTGCAGCTAAAAGAAGATTCATGGCCCAAACAGTCTTAGAATTAGGCTATGGGGGACAAACCCTTGCTGCACAGGAGTTAGGCTGGAATCGA
ACTACTATTCGTAAAGGAATTAAAGAACTAAAAAGAGGTATTATTTGTGTTGATAATCATTCAGCTAAGGGGCGGAAAAAAGCAGAAGAACATTTACCTT
TTCTATTGGAAAACATCAAAAGTTTAGTGGATTCTCAAAGCCAAACTGACCCAAGTTTTAAAAGCCAAAGGCTTTATGTGAGACTGAGTGCGGCCGAAGT
CCGAAAGCAATTAATCTCTAAATATGGGTACAGTGATGAGGATTTACCGAGCGAGGAAACTATTCGGGTTAAATTAAATAACTTAGGTTATCGTCTGAAA
AGAGTCGCTAAAGTTTTACCTCAAAAAAAATTCCAGAAACCGAGGCAATCTTTGAGGAATTAGCTAACATTAATCGGGAAGCGGATGAAGACCCTACGAT
GTTACGTCTTAGTTTGGATGCCAAAGCCCGTGTTAATATTGGACTATTTGATCGAGGAGGTAAGAATAGAATAACTGTCGAAACAAACGATCATGATTTT
AATCCGAAAACAACCCTAACCCCTTACGGAATATTTATTCCAGAATTTGATGAGTTGTTTTTGTATTTCACTGCCTCCACAGTCACCAGTGACTTTATTG
TTGATATATTAGAAGATTTCTGGGAGTCGGAAAAATCTCGTTTTGAGAAAATTAAAACTTTGATAATTAATCAAGATAATGGACCAGAAAATAATTCGAG
ACGAACTCAGTTCATGAAACGTATAGTTGAGTTTTCCCAAAAATATCAAGTTAATATACGTTTAGCTTACTATCCCCCTTACCATAGTAAATATAATCCT
ATTGAACGAACCTGGGCTGTGTTAGAAAACCCTTGGAATGGGAGTATTTTAGATGAAATCGAAACGGCTTTGAAATTCGCCCAAACTATGACTTGGAAAG
GAAAACACCCGATTGTTAAGTTGATTACTGAAACTTATGAAAAAGGAGTAAAGCTTACTAAAAAAGCCATGGAAAAAATCGAAGAAAAAATCGAACGTCT
CACAGAATCAACGAATCAAGACTTTCCCGATTTGGGACAATGGTTTATTGATATCTATTATGATAAGACCTAGTTTGTAGTTAACTAAGATGGTTAGCTA
AAAACTGTCTTTGATTCTTTAACTTGTCCTTGTTATACTCGAAAAAGCTCGGAAGTATAAGAGTTTGTGCTGCCTATTTTTTCGGAAAAATAGGTTGTAA
TGGGAGACTCTTTGTTTTCCTAGACAAAAATAATTACTTTTTCAAAAAAACACTTTTCTATTTTTTTGTTGGGTATTTTATTCTCTGGAAGTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 513 bp | 170 aa | 151 | 663 | + | No |
Description : First part of the transposase
ORF sequence :
MELTDSLKKLLSETALQLKGAAKRRFMAQTVLELGYGGQTLAAQELGWNRTTIRKGIKELKRGIICVDNHSAKGRKKAEEHLPFLLENIKSLVDSQSQTD
PSFKSQRLYVRLSAAEVRKQLISKYGYSDEDLPSEETIRVKLNNLGYRLKRVAKVLPQKKFQKPRQSLRN
PSFKSQRLYVRLSAAEVRKQLISKYGYSDEDLPSEETIRVKLNNLGYRLKRVAKVLPQKKFQKPRQSLRN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 762 bp | 253 aa | 612 | 1373 | + | No |
Description : Second part of the transposase
ORF sequence :
SFTSKKIPETEAIFEELANINREADEDPTMLRLSLDAKARVNIGLFDRGGKNRITVETNDHDFNPKTTLTPYGIFIPEFDELFLYFTASTVTSDFIVDIL
EDFWESEKSRFEKIKTLIINQDNGPENNSRRTQFMKRIVEFSQKYQVNIRLAYYPPYHSKYNPIERTWAVLENPWNGSILDEIETALKFAQTMTWKGKHP
IVKLITETYEKGVKLTKKAMEKIEEKIERLTESTNQDFPDLGQWFIDIYYDKT
EDFWESEKSRFEKIKTLIINQDNGPENNSRRTQFMKRIVEFSQKYQVNIRLAYYPPYHSKYNPIERTWAVLENPWNGSILDEIETALKFAQTMTWKGKHP
IVKLITETYEKGVKLTKKAMEKIEEKIERLTESTNQDFPDLGQWFIDIYYDKT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1223 bp | 407 aa | 151 | 1373 | + | Yes |
Chemistry : Unknow
ORF sequence :
MELTDSLKKLLSETALQLKGAAKRRFMAQTVLELGYGGQTLAAQELGWNRTTIRKGIKELKRGIICVDNHSAKGRKKAEEHLPFLLENIKSLVDSQSQTD
PSFKSQRLYVRLSAAEVRKQLISKYGYSDEDLPSEETIRVKLNNLGYRLKRVAKVLPQKKIPETEAIFEELANINREADEDPTMLRLSLDAKARVNIGLF
DRGGKNRITVETNDHDFNPKTTLTPYGIFIPEFDELFLYFTASTVTSDFIVDILEDFWESEKSRFEKIKTLIINQDNGPENNSRRTQFMKRIVEFSQKYQ
VNIRLAYYPPYHSKYNPIERTWAVLENPWNGSILDEIETALKFAQTMTWKGKHPIVKLITETYEKGVKLTKKAMEKIEEKIERLTESTNQDFPDLGQWFI
DIYYDKT
PSFKSQRLYVRLSAAEVRKQLISKYGYSDEDLPSEETIRVKLNNLGYRLKRVAKVLPQKKIPETEAIFEELANINREADEDPTMLRLSLDAKARVNIGLF
DRGGKNRITVETNDHDFNPKTTLTPYGIFIPEFDELFLYFTASTVTSDFIVDILEDFWESEKSRFEKIKTLIINQDNGPENNSRRTQFMKRIVEFSQKYQ
VNIRLAYYPPYHSKYNPIERTWAVLENPWNGSILDEIETALKFAQTMTWKGKHPIVKLITETYEKGVKLTKKAMEKIEEKIERLTESTNQDFPDLGQWFI
DIYYDKT
Blast result :
Comments
ISMae28 is 44% aa similar to ISArch16.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Modified and submitted by ISfinder (2010)
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.