ISMae29
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010296 | ND | Microcystis aeruginosa | Microcystis aeruginosa NIES-843 |
DNA section
IS Length : 1220 bp
Ends
IR Length : 22/28
IRL : TAGAGCATCAATTTTGTTTTGAGGGATAAAAAAAGAGCTAATCTGGAAGA
IRR : TAGTCTGTCAAGTTTGAATTGAGGGATAGAGTTTTTTTAATTTAATACGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTCGTCCGA | CTAG | ATTGGGCTGA | 4 |
| TCTTAATAGC | ATAC | ATCAGATCAA | 4 |
| ATTAAGGCTA | GTAG | GAACAGTGCT | 4 |
DNA sequence
TAGAGCATCAATTTTGTTTTGAGGGATAAAAAAAGAGCTAATCTGGAAGAAGTTATCCAACTTCAGACAAAAAAATGCCGAAGAAAAAATATATTGTAGA
GCTAACAGACTCCGAAAGAACTGAACTCGAGCAGCTAACCAAGAAAGGAAAAATAGCAGCTTACAAAATGAACCATGCCAGAATACTACTTTTAGCAGAC
GTCAATCAACAGGAAGGTGGTTGGACTGACTCGAAAATTAGTGAAGCCTTAAATGTTGCTCAAGCCACCATTGAGAGAGTACGACAAAGATTTGTCGAGT
CAGGAATTGAATCATCATTAACTCGAAAAAAGCAAGAACATCGCCGTGCCAAAATTATCGATGGGGAAAAGGAAGCAGATCTGATTGCCATCGCTTGTAG
TGAAACACCAACGGGACGAGCAAAATGGACACTTCAAATGCTGGCAGATAAAATGGTCGAACTTGAATATGTGGAAAAAATATCACGAGAAACCATCAGA
AAAACCTTAAAAAAAATGAATTGAAACCATGGCTGAATCAATCATGGGTCATTCCCCCAAAAGAATCAGCGGAGTTTGTCTGTGCCATGGAAGATGTCTT
GGATATTTATCATAGTGAGTATGACCAGCAAAATCCCTGGCTGTGTTTTGATGAGAGTTGTCAACAATTAGTCAAAGAAACAAAAGAGAAAATACCAGCC
GAACCGAGAGAACCAGAAAGGTATGACTATCAATATGAGCGTAATGGTGTAGCCAATATGTTTATGTTTTTTGAACCATTACAAGGTTGGCGACATGTAG
AAGTTACACAACAAAGGACGGCGAGCGATTATGCACACCAAATGAAATATTTGGTTGATGAGCGTTATCCGGATGCCCAGAAAATTAGAGTAATTCAAGA
CAACTTAAATACTCATGTAAAAGCCTCATTATATAAAGCCTTTGAACCGGAAGAGGCCAAGAGAATTTTAGATAAACTCGAATTTCATTACACTCCCAAA
CATGGCAGCTGGTTAAATATGGCAGAAATTGAGTTGAGCGTTTTGAATCGTCAATGTCTCGAGCGTCGTATCCCAGACATGGATAGTTTAAAACAAGAAA
TTAAGGCATGGGAAGAAAATCGAAATGAAAAGTCAAATTCAGTTGACTGGAGATTTACTACTGCTGATGCTCGTATTAAATTAAAAAAACTCTATCCCTC
AATTCAAACTTGACAGACTA
GCTAACAGACTCCGAAAGAACTGAACTCGAGCAGCTAACCAAGAAAGGAAAAATAGCAGCTTACAAAATGAACCATGCCAGAATACTACTTTTAGCAGAC
GTCAATCAACAGGAAGGTGGTTGGACTGACTCGAAAATTAGTGAAGCCTTAAATGTTGCTCAAGCCACCATTGAGAGAGTACGACAAAGATTTGTCGAGT
CAGGAATTGAATCATCATTAACTCGAAAAAAGCAAGAACATCGCCGTGCCAAAATTATCGATGGGGAAAAGGAAGCAGATCTGATTGCCATCGCTTGTAG
TGAAACACCAACGGGACGAGCAAAATGGACACTTCAAATGCTGGCAGATAAAATGGTCGAACTTGAATATGTGGAAAAAATATCACGAGAAACCATCAGA
AAAACCTTAAAAAAAATGAATTGAAACCATGGCTGAATCAATCATGGGTCATTCCCCCAAAAGAATCAGCGGAGTTTGTCTGTGCCATGGAAGATGTCTT
GGATATTTATCATAGTGAGTATGACCAGCAAAATCCCTGGCTGTGTTTTGATGAGAGTTGTCAACAATTAGTCAAAGAAACAAAAGAGAAAATACCAGCC
GAACCGAGAGAACCAGAAAGGTATGACTATCAATATGAGCGTAATGGTGTAGCCAATATGTTTATGTTTTTTGAACCATTACAAGGTTGGCGACATGTAG
AAGTTACACAACAAAGGACGGCGAGCGATTATGCACACCAAATGAAATATTTGGTTGATGAGCGTTATCCGGATGCCCAGAAAATTAGAGTAATTCAAGA
CAACTTAAATACTCATGTAAAAGCCTCATTATATAAAGCCTTTGAACCGGAAGAGGCCAAGAGAATTTTAGATAAACTCGAATTTCATTACACTCCCAAA
CATGGCAGCTGGTTAAATATGGCAGAAATTGAGTTGAGCGTTTTGAATCGTCAATGTCTCGAGCGTCGTATCCCAGACATGGATAGTTTAAAACAAGAAA
TTAAGGCATGGGAAGAAAATCGAAATGAAAAGTCAAATTCAGTTGACTGGAGATTTACTACTGCTGATGCTCGTATTAAATTAAAAAAACTCTATCCCTC
AATTCAAACTTGACAGACTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 450 bp | 149 aa | 75 | 524 | + | No |
Description : First part of the transposase
ORF sequence :
MPKKKYIVELTDSERTELEQLTKKGKIAAYKMNHARILLLADVNQQEGGWTDSKISEALNVAQATIERVRQRFVESGIESSLTRKKQEHRRAKIIDGEKE
ADLIAIACSETPTGRAKWTLQMLADKMVELEYVEKISRETIRKTLKKMN
ADLIAIACSETPTGRAKWTLQMLADKMVELEYVEKISRETIRKTLKKMN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 747 bp | 248 aa | 467 | 1213 | + | No |
Description : Second part of the transposase
ORF sequence :
ICGKNITRNHQKNLKKNELKPWLNQSWVIPPKESAEFVCAMEDVLDIYHSEYDQQNPWLCFDESCQQLVKETKEKIPAEPREPERYDYQYERNGVANMFM
FFEPLQGWRHVEVTQQRTASDYAHQMKYLVDERYPDAQKIRVIQDNLNTHVKASLYKAFEPEEAKRILDKLEFHYTPKHGSWLNMAEIELSVLNRQCLER
RIPDMDSLKQEIKAWEENRNEKSNSVDWRFTTADARIKLKKLYPSIQT
FFEPLQGWRHVEVTQQRTASDYAHQMKYLVDERYPDAQKIRVIQDNLNTHVKASLYKAFEPEEAKRILDKLEFHYTPKHGSWLNMAEIELSVLNRQCLER
RIPDMDSLKQEIKAWEENRNEKSNSVDWRFTTADARIKLKKLYPSIQT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1139 bp | 379 aa | 75 | 1213 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKKKYIVELTDSERTELEQLTKKGKIAAYKMNHARILLLADVNQQEGGWTDSKISEALNVAQATIERVRQRFVESGIESSLTRKKQEHRRAKIIDGEKE
ADLIAIACSETPTGRAKWTLQMLADKMVELEYVEKISRETIRKTLKKNELKPWLNQSWVIPPKESAEFVCAMEDVLDIYHSEYDQQNPWLCFDESCQQLV
KETKEKIPAEPREPERYDYQYERNGVANMFMFFEPLQGWRHVEVTQQRTASDYAHQMKYLVDERYPDAQKIRVIQDNLNTHVKASLYKAFEPEEAKRILD
KLEFHYTPKHGSWLNMAEIELSVLNRQCLERRIPDMDSLKQEIKAWEENRNEKSNSVDWRFTTADARIKLKKLYPSIQT
ADLIAIACSETPTGRAKWTLQMLADKMVELEYVEKISRETIRKTLKKNELKPWLNQSWVIPPKESAEFVCAMEDVLDIYHSEYDQQNPWLCFDESCQQLV
KETKEKIPAEPREPERYDYQYERNGVANMFMFFEPLQGWRHVEVTQQRTASDYAHQMKYLVDERYPDAQKIRVIQDNLNTHVKASLYKAFEPEEAKRILD
KLEFHYTPKHGSWLNMAEIELSVLNRQCLERRIPDMDSLKQEIKAWEENRNEKSNSVDWRFTTADARIKLKKLYPSIQT
Blast result :
Comments
ISMae29 is 75% aa similar to ISAcma29.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Modified and submitted by ISfinder (2010)
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.