ISNpu13

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) TnNpu13
Accession numberTranspositionOriginHost
NC_010633 ND Nostoc punctiforme
Nostoc punctiforme PCC 73102 plasmid pNPUN04
DNA section
IS Length : 4823 bp

Ends


IR Length : 49

IRL : GGGGTAAATATAGCGAAGCTGTCACCAGGGGCAAGATAGGCTGAAAGTAT
IRR : GGGGTAAATATAGCGAAGCTGTCACCAGGGGCAAGATAGGCTGAAAGTAC

Insertion site


Left flankDirect repeatRight flankDR Length
CCCTGAACCCCCCCCGCAATACGGAAAGTGAAAAGAGGGGTG5

DNA sequence

GGGGTAAATATAGCGAAGCTGTCACCAGGGGCAAGATAGGCTGAAAGTATTGTGGAGTCTGGGTTTGAGAAAAAGAGTTGATTATTGGATTTTAGCGGTT
TTGACAGGCGTTTTATAAGCACAGCCACAAATTGGACAAAATCGGTGTCTCAAAGACAACACCAACTCACCGCAATTCGCACAGTTAGCTCGAATCGGTG
TACCGCAGAGATAACAAAACTTGGCTTTGATATGACTCCACATAGGGTCAACCGCCGCGCCAGGATTCCAACATTGGGGACAAAACTTAACCCCTCTAAC
CTGAGATACTTCCTCACCCTTGATCACAGCATCAAAATATTCTTGAGGTACGCCAAGTGCGATCGCTAACCCTTGAAGTGTCCTACGATTAATTTTAAGC
GTAAGTCCTCGCTCGATTTTCCCGACAGAGCGTCCATGTATACCAGCAGCATCCGCCAACTCAGACTGAGTTAAATTGAGTTTCTTCCTGACCCGTGACA
CGTAACTAGCAAGGGATTCATCGCTATTGGGAGTTTTAAAAGTATCCATAAAAAGCTCAGGGCTAACTCATAAAAAGATATATAATTTATCAATAAAAAA
CCAAGCGAGAGAATACAGAAACAACCGTGAAATCGGCTGTAACTTTAGCCACTGTCACGACCGAATTTCTAGAGCGTCCTGGTCTAGCACCAAGTACTAG
AGAAACCTATGAACTGACCCTCGGACTTTTACTGCAAAAGTATGGAAGTTGGTCAATAGAAATTATCAGTAAGCAAACATTAGTTGAGTATCTAAATAAT
CTCAGTCATTTAAAATACACAACACACCACAAGCATCAAGCAATATTACAAAGTTTGCTTAACTTTGCAGTAGAACAAGGGTATATAAAATCCAACCCTA
TCCGGGGACTAAAACAACGTCTACCTGAAAGAGAGAAAGGCGAACATAAAAGCGACGAGACAATAAGATATCTAACACAATCACAACTAAATATACTATA
TTCTGCCGTAAAAGATGACTTACGTCTAAGTACGATAGTTCATTTACTGCATCGCACAGGATGCAGAATAGGAGAGCTTTTAGCCCTAAACATAGATTCT
GTAGATATCAAGAACCAGAAATTTCAAGTATTAGGTAAAGGCAACAAACAACGCTGGTGTTTTTACAGTGATGACGCAGCCTCAATGCTTGCTAAATACT
TGAAATACTCACGGCATCAAAATACGAATGCCTTGTTTACAGCACAACATCCAGTTACCCGAAAAGTAAGTAGAATCAGCTACCATACATTGCATGATTA
TTGGCGGGAGACAACCAATAAGTATCCACACCTTCAAGGAGTTCGCATTCACGATTTACGACATACATTTGCTACAGAACGAGTGGGGTTAATCAGTATT
GAGGAATTATGCTCATTAATGGGACATGAAAGCATTCAAACAACTTTACGTTACGCTAAAGTCACCTCACAGAAATCAGAATCAGCTGCACGTCATGCCT
TGAATATACTGATTAATTCCGACCAAAAAGCTGACCTTTAGGGCAATTATATAGAATCACTCAGTTCCAGTATGTTTTTCATCGAGAAACAGCATTTTAT
CTCAGTTAAAGATTGTTACCCCAAAGAAGATAGAATACTGTTTGGTTGAAGAGTGAGACTTAATGAAAAAAACCTGGCAGCCAGAAGAATTAGTAGAGTA
TTGGACGCTACTCCCTCTTGAATTAGCACTACTGAATCAGAAAAACGACGAAAATCGGTTAGGTTTTGCTATATTGCTCAAGTTTTTTGAGATTTATGCC
AGATTTCCTGAGAATAATCAAGAAATCTCCCAAAATGTTGTTGATTATGTTGCCAAACTTTTAGATATTTCCCCCCAAAAGTACTCTTACTACGATTGGC
AAGGACGCTCCATAAAATATCATCGCGCTCAAATTAGAGAATTCTTTGGATTTCAAGAAACCAAAGTTAGTGATGCTTTTGAGTTAACTAATTGGTTAGC
AGAACAAGCACTAATTTATGAGTTAAAATTTGAACAGCTAAAACTTGCAGCAGTAGCGAGACTACGGAATTTAAAACTTGAACCACCGACAGATTCCAGA
TTAGAACGTATCATTCGCTCTGCTATCAGAAATTTTGAATCTAAATTTTTTCGGGACATAACTTCCCAACTATCTAACCAATCTAAAAAGTGGATAAATG
ATTTTTTGAAAGAGAAAGCTTCCGATCAGGAATTAGATGATACCACACAAACAAATCATGATAAACAACTGAATAATCCAGGAGATATCCAATTATCTCA
ACAGTTAGATGACACGAATAACTCAGAAATAAACACTGAAGTGACTAGATTAACATGGGCTGAATTAAAAACAGACCCAGGGAGAATTGGTGTAGAAAGT
TTTCAAAAGGAAGTTGCTAAATTAGAGATTTTTGAGCAATTAGAATTACCAACAGACTTATTTAAAAACATCTCTACCAAAGTTCTCCAAGTCTATAAAA
GTCGGGTAATTGTTGAACATCCTCGTGACTTACGCCGTCACCCAGAACATATTCGCTACACACTATTTACAGCTTTTTGTTGGCTCAGAAGTCAAGAAGT
TATAGATAATTTAGTAGAGTTACTCATTCAGATTGTCCATCGCATAGGTGTGAATGCAGAAAAACGTGTAGATAAAGAGCTTATTCAGGATTTTAAACGG
GTTCATGGGAAGAATAATCTACTATACCAAATGGCAGAAGCTTCCTTGAAAACACCTGATGGCACAGTCAAGGATGTGATTTTTCCTGTAGTAAGTGAAG
TGACTCTCAAGAATTTAGTGAAAGAGTATAAATCTACAGGGAATGCTTACCGTGAGAAAGTTTATACGGTGATGCGCTCTTCTTATGGTGGATATTATCG
GCGAATTTTACCGTTAATTTTAAACAAGCTCGAATTTCGTTCAAATAATGAAGTTCATCGTCCAGTTATTCAAGCGATATCTTTACTAAAAAAGTATGCT
GATAGTAAACAGCGTTATTATGATGCTGGAGAAGATATACCCATTGAGGGGGTATTACGTAGTGGTTGGCAAGAAATTATATTAGAGACGGGAGCAGACG
ATGAAATCAAAGTTAATCGCATTAATTATGAGTTGAGCGTGCTGCAAGCATTGAGAGAAAAGCTGAGATGCAAGGAAATATGGGTTGTAGGAGCTAATCG
TTACAGAAACCCTGAAGAAGATTTACCTGCTGATTTTGAGGCGCAAAGATTAGAATATTTCCAAGCTTTGAAACAACCATCCGATGTAGAAGTCTTTATT
GCTAATTTACAGCAATCTTTGGAGCAATCATTAACTTTACTGGATAAAGGAATACCTAAAAATAGTTTAGTCACACTTAAAACGACAGGCAAAAGTCGAA
TTTGTGTTTCTCCTCTGAATCCTCAAAATGAACCGACTAATTTGACTAGGTTGAAAACTGAAATTACTAGGCGTTGGCGCATGACTAGCCTCTTAGATAT
TCTGAAAGAGACTGATTTGCGAGTTAAGTTTAGCCAACACTTTCAGAGTGTATCAACACGAGAGGTTTTAGGCGAATATACTTTACAAAAACGCTTGCTA
CTTTGTTTATATGGTTTAGGAACGAATACTGGATTAAAGCGTTTAAGTGATGAGATTAAAGGCGACAATTATCAAGATTTACTCCATGTTAAGCGTCATT
TTATTCAGAAAGAACAATTACGTAATGCTATTGCCTGTATTGCAAATGCAATTTTTACTGCCAGAATTTCTACTATTTGGGGAGAGGGTACAACCGCCTG
TGCTTCTGACTCGAAAAAGTTTGGTGCTTGGGATCAAAACTTAATGACTGAGTGGCACATCCGTTATGGTGGAAGAGGTGTGATGATTTATTGGCACGTT
GAGAAGAATTCTGTTTGTATTTATTCACAGTTAAAAACTTGTTCTTCTTCTGAAGTGGCAGCCATGATTGAAGGATTATTACGCCACTGTACTGAGATGA
AAGTGGAGACAAATTATGTGGATAGTCACGGTCAAAATGAAGTGGCTTTTGCTTTCTGTCATTTACTGGGTTTTCAGTTAATGCCGCGTCTTAAACGTAT
CAATGTTCAGAAATTATACCGTCCTTCAACTGGAAATAATGATGCTTATCCAAACTTGCAACCTATTTTAACTCGCGCTATTAATTGGGATTTAATTCGC
CAACAATACGACCAAATGGTGAAGTATGCTACTGCTTTGCGTTTGGGTATGGCAGAAACTGAAGCCATCTTAAAGCGTTTTACTAGGGGTAATTTATTAC
ACCCTACTTATCAAGCCTTGGCGGAATTGGGCAAGGCTGTAAAAACTATATTTTTATGTAAATACCTGCATTCGGTTGAACTGCGACAAGAGATAAATGC
TGGACTCAACGTGGTGGAGAACTGGAATAGTGCTAACAGTTTTATTTTTTACGGCAAGGGTGGGGAGATTGCTACTAATCGTTTGTCTGACCAAGAATTA
GCTGTGTTATCTCTGCATTTACTCCAGATATCTTTGGTATATATAAATACGTTGATGATTCAGGAGGTTTTGTCCCAACCACAATGGATGAAGTTGATGA
AGTTGGAGGATTTGCGGGCGCTTTCGCCTTTAATTTGGGGTCACGTTAATCCCTATGGTACTTTTCGTTTGGATCTTAATGTCAGGTTGCCAATTGAGAC
GGGGTAAATGTGTCTAAAAATACGCTTTGTTCGTATGTCTCTTAATCTTATAGTCTGAAAGTCATCCACCGCAGTACTTTCAGCCTATCTTGCCCCTGGT
GACAGCTTCGCTATATTTACCCC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
468 bp155 aa54982-No
ORF function : Passenger Gene
Annotation : XRE family transcriptional regulatorDescription : Transcriptional Regulator factor

ORF sequence :

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
915 bp304 aa6271541+No
ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MKSAVTLATVTTEFLERPGLAPSTRETYELTLGLLLQKYGSWSIEIISKQTLVEYLNNLSHLKYTTHHKHQAILQSLLNFAVEQGYIKSNPIRGLKQRLP
EREKGEHKSDETIRYLTQSQLNILYSAVKDDLRLSTIVHLLHRTGCRIGELLALNIDSVDIKNQKFQVLGKGNKQRWCFYSDDAASMLAKYLKYSRHQNT
NALFTAQHPVTRKVSRISYHTLHDYWRETTNKYPHLQGVRIHDLRHTFATERVGLISIEELCSLMGHESIQTTLRYAKVTSQKSESAARHALNILINSDQ
KADL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
3045 bp1014 aa16634707+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKTWQPEELVEYWTLLPLELALLNQKNDENRLGFAILLKFFEIYARFPENNQEISQNVVDYVAKLLDISPQKYSYYDWQGRSIKYHRAQIREFFGFQET
KVSDAFELTNWLAEQALIYELKFEQLKLAAVARLRNLKLEPPTDSRLERIIRSAIRNFESKFFRDITSQLSNQSKKWINDFLKEKASDQELDDTTQTNHD
KQLNNPGDIQLSQQLDDTNNSEINTEVTRLTWAELKTDPGRIGVESFQKEVAKLEIFEQLELPTDLFKNISTKVLQVYKSRVIVEHPRDLRRHPEHIRYT
LFTAFCWLRSQEVIDNLVELLIQIVHRIGVNAEKRVDKELIQDFKRVHGKNNLLYQMAEASLKTPDGTVKDVIFPVVSEVTLKNLVKEYKSTGNAYREKV
YTVMRSSYGGYYRRILPLILNKLEFRSNNEVHRPVIQAISLLKKYADSKQRYYDAGEDIPIEGVLRSGWQEIILETGADDEIKVNRINYELSVLQALREK
LRCKEIWVVGANRYRNPEEDLPADFEAQRLEYFQALKQPSDVEVFIANLQQSLEQSLTLLDKGIPKNSLVTLKTTGKSRICVSPLNPQNEPTNLTRLKTE
ITRRWRMTSLLDILKETDLRVKFSQHFQSVSTREVLGEYTLQKRLLLCLYGLGTNTGLKRLSDEIKGDNYQDLLHVKRHFIQKEQLRNAIACIANAIFTA
RISTIWGEGTTACASDSKKFGAWDQNLMTEWHIRYGGRGVMIYWHVEKNSVCIYSQLKTCSSSEVAAMIEGLLRHCTEMKVETNYVDSHGQNEVAFAFCH
LLGFQLMPRLKRINVQKLYRPSTGNNDAYPNLQPILTRAINWDLIRQQYDQMVKYATALRLGMAETEAILKRFTRGNLLHPTYQALAELGKAVKTIFLCK
YLHSVELRQEINAGLNVVENWNSANSFIFYGKGGEIATNRLSDQELAVLSLHLLQISLVYINTLMIQEVLSQPQWMKLMKLEDLRALSPLIWGHVNPYGT
FRLDLNVRLPIETG

 

Blast result :
Comments
ISNpu13 (ORF3 : transposase) is 66% aa similar to ISYps3.
This IS had 3 ORFS, ORF1 : XRE family transcriptional regulator, ORF2 : recombinase and ORF3 : transposase.
References
1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Diret submission GenBank.