ISNml3
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013235 | ND | Nakamurella multipartita | Nakamurella multipartita DSM 44233 |
DNA section
IS Length : 1798 bp
Ends
IR Length : 14/15
IRL : GACGCTCCTGAATTATCCGTATGTCGGCCCCTGTTGATCTTGTGGGTTCG
IRR : GACGCTCCTGAATAACGGTCACTTGTGGGTTCGGGAGTTCGCTTTGGGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGGCCGGAT | CTAG | ACGCCCTCGC | 4 |
| CGCCGGCCCG | CTAG | AACCCGGACG | 4 |
| CGGAGTCTAG | CTTGAGATGT | 0 | |
| TGGGGCGGTT | CTAG | AGCGTATCGC | 4 |
| GCCCGGCCGG | CTAG | TGCGTGGTCG | 4 |
| CCGGCCGGAT | CTAG | ACGCCCTCGC | 4 |
| GTCCCAGCCC | CTAG | AGGACATCCA | 4 |
| GCGCGGGGGG | CTAG | AGCGAGCGCC | 4 |
| CAAAGTACTA | CTAG | CCTCGATCTT | 4 |
| CAGAGTGCTT | CTAG | AGACCCTCGG | 4 |
| GTCAGTACAG | CTAG | ACGGTATGGG | 4 |
| AGCGGACCGG | CTAG | AGCGCGGCGC | 4 |
DNA sequence
GACGCTCCTGAATTATCCGTATGTCGGCCCCTGTTGATCTTGTGGGTTCGGGATGATCTTGGTGTGCAGGGTCGGTCGCGGGATCAGCGTGAGTTGTTGG
ATGCCGAGTCGGTAGTCGGTGGGCTGCTCAAGCCGGGCAGCGTGTTCGCGTTTCTGGCCGCGCACCGTCGGGAGGTGTTCCCGGACGGCATGTTCGCGGA
TCTGTTTCCGTCGGGTCGGGGCCGCCCGTCAGTGCCGGCCGACGTGATGGCGTCGGTGATTGTGCTGCAGGCTCTGCACGGCCTGTCCGACGCGGACACG
GTGGACTCGGTGACGTTCGATCTGCGGTGGAAGGCAGCGTGCGGGTTACCGGTGACCGCTGCGGCGTTCCATGCCACGACATTGACGTACTGGCGGCGTC
GGCTGGCCGCTTCGCAGTCGCCGAACCGGATCTTCGACGCGGTCCGCCAGGTCGTGGACCAGACCGGGGTGCTGGCTGGAAAGAGCAGGCGAGCGTTGGA
TTCCACGATCCTGGACGACGCGGTCGCCACCCAGGACACGGTCACCCAGTTGATCGCGGCGATCCGCCGGGTCCGCCGCGAGGTACCCGGCGCCGCCGAG
GTCGTCGGCGAGCACTGCTCGGCTCACGACTATGACGACCCGGGCAAACCGGCGATCGCCTGGAACGATCAGCAGGCCCGCGAGGCCCTTGTCGATGCGC
TGGTCACCGACGCGCATCGGGTGCTGGGACACCTGCCCGACCAGGAGCTCGGACCGAAGGCGGCGGACGCGGTCGCCCTCTTGGCGTTGGTCGCCGGGCA
GGACGTGGAACCGGTCGAGGGCTCGGACGGCACCGACGGACGGTGGCGGATCGCGCAGCGGGTCGCCCCGGACCGGGTGATCTCCACCGTGGACCCGGAG
GCGCGGCACGCCCACAAGACCGTCCACCGGCGGCAGGACGGGTTCAAGGCACACATCGCGGTCGAACCCGACACCGGTCTGGTCACCGCCTGCGCGGTGA
CCATGGCCAGCGGACGCGGCAACAGCGACGCCGAGGTTGGACCCACCTTGCTGGCACAGGAGACCGAAAAGCTGCACGTGCTGGCCGATTCGGCGTACGG
ATCGGGATCCGCGCGGGCCGAACTGGACCATGCCGGGCACATCGCGTTGATCAAGCCGTTCCCGCTGCGGTCGGCCGTGCCGGGCGGGTTCACCCTGGAC
GACTTCACCGTCGACCCCGAGGCCAGGACGGCCACCTGCCCGAACGGGGTGACCCGGTCGATCACCGCGCAATGGTCCGTCACCTTCGGAGCGGCTTGCC
GCGGCTGCCCGCTCCGGGCCCAATGCACGACCAGCGACGCCGGTCGATCGCTGAAGCTGACCGAGTACGAAAGCCTGCTCAGGGCGGCCCGTCGACAAGC
GGAAACCGAGGACTTCCAACAGGTCTACCGACGGCACCGGCCGATGGTCGAACGATCGATCTCCTGGCTGGTCCGCGGCAACCGCAAAGTCCGCTACCGC
GGCGTCGCCAAGAACGACCACTGGTGGCACCACCGCGCCGCTGCGATCAACCTCAGGCGAATGCTCACCCTCGGGCTGACGCGGGTGAGCGGGACGTGGA
CCATTGCACCGGCCTGACCGGCCGGCACGAGACCTCACCGGGCCGTGAGCAACGGCCCGTCCCTCCGATCAGTCAGGAGAATCATCCAGGGCCGGGCGAC
GACTCAGGACCACGCCTGACAGCCCGATCACGCGCGAACGCTCCCACTGTCCCAAAGCGAACTCCCGAACCCACAAGTGACCGTTATTCAGGAGCGTC
ATGCCGAGTCGGTAGTCGGTGGGCTGCTCAAGCCGGGCAGCGTGTTCGCGTTTCTGGCCGCGCACCGTCGGGAGGTGTTCCCGGACGGCATGTTCGCGGA
TCTGTTTCCGTCGGGTCGGGGCCGCCCGTCAGTGCCGGCCGACGTGATGGCGTCGGTGATTGTGCTGCAGGCTCTGCACGGCCTGTCCGACGCGGACACG
GTGGACTCGGTGACGTTCGATCTGCGGTGGAAGGCAGCGTGCGGGTTACCGGTGACCGCTGCGGCGTTCCATGCCACGACATTGACGTACTGGCGGCGTC
GGCTGGCCGCTTCGCAGTCGCCGAACCGGATCTTCGACGCGGTCCGCCAGGTCGTGGACCAGACCGGGGTGCTGGCTGGAAAGAGCAGGCGAGCGTTGGA
TTCCACGATCCTGGACGACGCGGTCGCCACCCAGGACACGGTCACCCAGTTGATCGCGGCGATCCGCCGGGTCCGCCGCGAGGTACCCGGCGCCGCCGAG
GTCGTCGGCGAGCACTGCTCGGCTCACGACTATGACGACCCGGGCAAACCGGCGATCGCCTGGAACGATCAGCAGGCCCGCGAGGCCCTTGTCGATGCGC
TGGTCACCGACGCGCATCGGGTGCTGGGACACCTGCCCGACCAGGAGCTCGGACCGAAGGCGGCGGACGCGGTCGCCCTCTTGGCGTTGGTCGCCGGGCA
GGACGTGGAACCGGTCGAGGGCTCGGACGGCACCGACGGACGGTGGCGGATCGCGCAGCGGGTCGCCCCGGACCGGGTGATCTCCACCGTGGACCCGGAG
GCGCGGCACGCCCACAAGACCGTCCACCGGCGGCAGGACGGGTTCAAGGCACACATCGCGGTCGAACCCGACACCGGTCTGGTCACCGCCTGCGCGGTGA
CCATGGCCAGCGGACGCGGCAACAGCGACGCCGAGGTTGGACCCACCTTGCTGGCACAGGAGACCGAAAAGCTGCACGTGCTGGCCGATTCGGCGTACGG
ATCGGGATCCGCGCGGGCCGAACTGGACCATGCCGGGCACATCGCGTTGATCAAGCCGTTCCCGCTGCGGTCGGCCGTGCCGGGCGGGTTCACCCTGGAC
GACTTCACCGTCGACCCCGAGGCCAGGACGGCCACCTGCCCGAACGGGGTGACCCGGTCGATCACCGCGCAATGGTCCGTCACCTTCGGAGCGGCTTGCC
GCGGCTGCCCGCTCCGGGCCCAATGCACGACCAGCGACGCCGGTCGATCGCTGAAGCTGACCGAGTACGAAAGCCTGCTCAGGGCGGCCCGTCGACAAGC
GGAAACCGAGGACTTCCAACAGGTCTACCGACGGCACCGGCCGATGGTCGAACGATCGATCTCCTGGCTGGTCCGCGGCAACCGCAAAGTCCGCTACCGC
GGCGTCGCCAAGAACGACCACTGGTGGCACCACCGCGCCGCTGCGATCAACCTCAGGCGAATGCTCACCCTCGGGCTGACGCGGGTGAGCGGGACGTGGA
CCATTGCACCGGCCTGACCGGCCGGCACGAGACCTCACCGGGCCGTGAGCAACGGCCCGTCCCTCCGATCAGTCAGGAGAATCATCCAGGGCCGGGCGAC
GACTCAGGACCACGCCTGACAGCCCGATCACGCGCGAACGCTCCCACTGTCCCAAAGCGAACTCCCGAACCCACAAGTGACCGTTATTCAGGAGCGTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1554 bp | 517 aa | 64 | 1617 | + | No |
Chemistry : DDE
ORF sequence :
MQGRSRDQRELLDAESVVGGLLKPGSVFAFLAAHRREVFPDGMFADLFPSGRGRPSVPADVMASVIVLQALHGLSDADTVDSVTFDLRWKAACGLPVTAA
AFHATTLTYWRRRLAASQSPNRIFDAVRQVVDQTGVLAGKSRRALDSTILDDAVATQDTVTQLIAAIRRVRREVPGAAEVVGEHCSAHDYDDPGKPAIAW
NDQQAREALVDALVTDAHRVLGHLPDQELGPKAADAVALLALVAGQDVEPVEGSDGTDGRWRIAQRVAPDRVISTVDPEARHAHKTVHRRQDGFKAHIAV
EPDTGLVTACAVTMASGRGNSDAEVGPTLLAQETEKLHVLADSAYGSGSARAELDHAGHIALIKPFPLRSAVPGGFTLDDFTVDPEARTATCPNGVTRSI
TAQWSVTFGAACRGCPLRAQCTTSDAGRSLKLTEYESLLRAARRQAETEDFQQVYRRHRPMVERSISWLVRGNRKVRYRGVAKNDHWWHHRAAAINLRRM
LTLGLTRVSGTWTIAPA
AFHATTLTYWRRRLAASQSPNRIFDAVRQVVDQTGVLAGKSRRALDSTILDDAVATQDTVTQLIAAIRRVRREVPGAAEVVGEHCSAHDYDDPGKPAIAW
NDQQAREALVDALVTDAHRVLGHLPDQELGPKAADAVALLALVAGQDVEPVEGSDGTDGRWRIAQRVAPDRVISTVDPEARHAHKTVHRRQDGFKAHIAV
EPDTGLVTACAVTMASGRGNSDAEVGPTLLAQETEKLHVLADSAYGSGSARAELDHAGHIALIKPFPLRSAVPGGFTLDDFTVDPEARTATCPNGVTRSI
TAQWSVTFGAACRGCPLRAQCTTSDAGRSLKLTEYESLLRAARRQAETEDFQQVYRRHRPMVERSISWLVRGNRKVRYRGVAKNDHWWHHRAAAINLRRM
LTLGLTRVSGTWTIAPA
Blast result :
Comments
ISNml3 is 80% aa similar to ISMgi2.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Ovchinnikova,G., Sims,D., Meincke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Ovchinnikova,G., Sims,D., Meincke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.