ISMgi2
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009339 | ND | Mycobacterium gilvum | Mycobacterium vanbaalenii PYR-1 Mycobacterium gilvum PYR-GCK plasmid pMFLV01 |
DNA section
IS Length : 1770 bp
Ends
IR Length : 13/15
IRL : GAGTCTGCTGAATTACTCCGGCGTGGTGTCGGGGTTTGCGTCTGGGTTCT
IRR : GACTGTGCTGAATTAGGGGGTTCGGGGCGGGGTGCCTCAAAGCGCTCTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCGCGGCGC | CTAG | GCGTGCACGG | 4 |
| CACTCAGGTG | CTAG | AGCTGGCCGC | 4 |
| CCCTCGTGCG | CTAG | GCCTGGTCGC | 4 |
| CCGGTTACCG | CTAG | CACCCAATCG | 4 |
| GCTTGAGCGC | CTAG | CGGCCAACGC | 4 |
DNA sequence
GAGTCTGCTGAATTACTCCGGCGTGGTGTCGGGGTTTGCGTCTGGGTTCTCGAGATGATTGAGGCGTGCAGGGACACTCTGATGATCAGCGTGAGTTGTT
GGATGCGGAATCGATCGCTGGTCATCTTCTGAAGTCCGACAGCATGTTTGCGTTCCTGGCCGCACACCGCCATGAACTGTTCCCGGAGGAGATGTTCGCC
GATCTGTTCCCCTCGCAGCGGGGCCGTCCGAGCATCCCGGCTGAGGTGATGGCCTCGGTCATTACTCTGCAGGCCCTGCACGGGTTGTCCGATAACGAGA
CCGTAGACGCCGTCACCTTCGATCTGCGGTGGAAGGCCGCATGCGGGCTGCCGGTCACGGCGGGGGCGTTTCATTCGACGACGCTGACCTATTGGAGGCG
CCGGCTGGTGGCCTCAGATCGTCCGAACCGGATCTTCGAAGCTGTCAAGGCGGTGGTCACCGCGACCGGGGTATTGGCCAAAAAGACGCGACGAGCGTTG
GATTCCACGGTTCTTGATGACGCGGTGGCCACTCAGGACACCGTGACCCAGCTGATCGCCGCGATCCGCCGGGTGGGCCGCGAGGTTTCCGGCGCGGGCG
AGGTGATCGCCGCTGTGTGCAGCGCCCACGATTACACCGATGCGGGTAAACCGGCGATCGCCTGGAACGACAAAACCGCCCGCGACCAGCTGGTCGACGG
CCTGGTTGGTGACGCCCACCGACTCCTGGGCCATCTGCCCGAACAGGATCTCGGGCCCCGCGCCGCGGAGGCGGTCGCGTTGTTGGCGCTGGTCGCCGGC
CAGGACGTCGAACCGGTTGAGGGCTCTGATGGCACCGACGGGCACTGGCAGATCGCGCAAAAAGTTGCCGGTGATCGGGTGATCTCGACCGTCGATCCCG
AGGCGCGGCACGCGCACAAGACCGTGCATCGGCGCCAGGACGGGTTCAAGGCCCACATCGCGGTCGAACCCGACACCGGGATCATCACCGACTGCGCCCT
GACTAAAGCCAGCGGCCCGGACAACCACGAAGCCGTCATCGGACTGGCCCTGCTGGCCGACGAGCACACCCCGGTGCGGGTGCTTGGCGATTCGGCCTAC
GGCACCGGCGACGCCCGGGCCGCGCTGGCCGAGCTCAAGCATGTCGCGGTGATCAAACCGCTGCCGTTGCGTGTGCCGGTACCTGGCGGCTTCACCAGCG
ATGACTTCATCATCGATTTCGACGCCCGCACCGTGACATGTCCGGCTGAGCACACAGTCGCTATTGGCCGCACTGGTGGGGCGGGGTTCGAAAAGCACTG
CCGGTCATGCCCTTTACGGCAGCAGTGCACCACCGCCAAGCGTGGACGCAAACTCACCATCAGTGAGCATGAACCCCACCTGCGCGCCGCCCGAGCGGTC
GCCCGCACACCCGAATGGCAGAACGAATACCGCCAACACCGTCCCATGGTCGAACGCTCTATCGCCTGGCTAGCCCGCGGAAACCGCAAAGTCCGCTACC
GCGGTGTCACCAAGAACGATCACTGGCTGCACCACCGTGTCGCCGCGTTGAACTTACGACGCCTGATCACCATCGGACTGATCCGCAACGGCACAACCTG
GGCCATCGCCTAGCGACAACCACTGGCAGGCCTTGCCAGCGCCAACCAACAAGCGCCACTCTCAACACGGCGGGGTGACCCTCGAGTGTCGACCCCAGGG
TCTGGCTGAACCCACGCGCCGCAGAGCGCTTTGAGGCACCCCGCCCCGAACCCCCTAATTCAGCACAGTC
GGATGCGGAATCGATCGCTGGTCATCTTCTGAAGTCCGACAGCATGTTTGCGTTCCTGGCCGCACACCGCCATGAACTGTTCCCGGAGGAGATGTTCGCC
GATCTGTTCCCCTCGCAGCGGGGCCGTCCGAGCATCCCGGCTGAGGTGATGGCCTCGGTCATTACTCTGCAGGCCCTGCACGGGTTGTCCGATAACGAGA
CCGTAGACGCCGTCACCTTCGATCTGCGGTGGAAGGCCGCATGCGGGCTGCCGGTCACGGCGGGGGCGTTTCATTCGACGACGCTGACCTATTGGAGGCG
CCGGCTGGTGGCCTCAGATCGTCCGAACCGGATCTTCGAAGCTGTCAAGGCGGTGGTCACCGCGACCGGGGTATTGGCCAAAAAGACGCGACGAGCGTTG
GATTCCACGGTTCTTGATGACGCGGTGGCCACTCAGGACACCGTGACCCAGCTGATCGCCGCGATCCGCCGGGTGGGCCGCGAGGTTTCCGGCGCGGGCG
AGGTGATCGCCGCTGTGTGCAGCGCCCACGATTACACCGATGCGGGTAAACCGGCGATCGCCTGGAACGACAAAACCGCCCGCGACCAGCTGGTCGACGG
CCTGGTTGGTGACGCCCACCGACTCCTGGGCCATCTGCCCGAACAGGATCTCGGGCCCCGCGCCGCGGAGGCGGTCGCGTTGTTGGCGCTGGTCGCCGGC
CAGGACGTCGAACCGGTTGAGGGCTCTGATGGCACCGACGGGCACTGGCAGATCGCGCAAAAAGTTGCCGGTGATCGGGTGATCTCGACCGTCGATCCCG
AGGCGCGGCACGCGCACAAGACCGTGCATCGGCGCCAGGACGGGTTCAAGGCCCACATCGCGGTCGAACCCGACACCGGGATCATCACCGACTGCGCCCT
GACTAAAGCCAGCGGCCCGGACAACCACGAAGCCGTCATCGGACTGGCCCTGCTGGCCGACGAGCACACCCCGGTGCGGGTGCTTGGCGATTCGGCCTAC
GGCACCGGCGACGCCCGGGCCGCGCTGGCCGAGCTCAAGCATGTCGCGGTGATCAAACCGCTGCCGTTGCGTGTGCCGGTACCTGGCGGCTTCACCAGCG
ATGACTTCATCATCGATTTCGACGCCCGCACCGTGACATGTCCGGCTGAGCACACAGTCGCTATTGGCCGCACTGGTGGGGCGGGGTTCGAAAAGCACTG
CCGGTCATGCCCTTTACGGCAGCAGTGCACCACCGCCAAGCGTGGACGCAAACTCACCATCAGTGAGCATGAACCCCACCTGCGCGCCGCCCGAGCGGTC
GCCCGCACACCCGAATGGCAGAACGAATACCGCCAACACCGTCCCATGGTCGAACGCTCTATCGCCTGGCTAGCCCGCGGAAACCGCAAAGTCCGCTACC
GCGGTGTCACCAAGAACGATCACTGGCTGCACCACCGTGTCGCCGCGTTGAACTTACGACGCCTGATCACCATCGGACTGATCCGCAACGGCACAACCTG
GGCCATCGCCTAGCGACAACCACTGGCAGGCCTTGCCAGCGCCAACCAACAAGCGCCACTCTCAACACGGCGGGGTGACCCTCGAGTGTCGACCCCAGGG
TCTGGCTGAACCCACGCGCCGCAGAGCGCTTTGAGGCACCCCGCCCCGAACCCCCTAATTCAGCACAGTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1548 bp | 515 aa | 66 | 1613 | + | No |
Chemistry : DDE
ORF sequence :
MQGHSDDQRELLDAESIAGHLLKSDSMFAFLAAHRHELFPEEMFADLFPSQRGRPSIPAEVMASVITLQALHGLSDNETVDAVTFDLRWKAACGLPVTAG
AFHSTTLTYWRRRLVASDRPNRIFEAVKAVVTATGVLAKKTRRALDSTVLDDAVATQDTVTQLIAAIRRVGREVSGAGEVIAAVCSAHDYTDAGKPAIAW
NDKTARDQLVDGLVGDAHRLLGHLPEQDLGPRAAEAVALLALVAGQDVEPVEGSDGTDGHWQIAQKVAGDRVISTVDPEARHAHKTVHRRQDGFKAHIAV
EPDTGIITDCALTKASGPDNHEAVIGLALLADEHTPVRVLGDSAYGTGDARAALAELKHVAVIKPLPLRVPVPGGFTSDDFIIDFDARTVTCPAEHTVAI
GRTGGAGFEKHCRSCPLRQQCTTAKRGRKLTISEHEPHLRAARAVARTPEWQNEYRQHRPMVERSIAWLARGNRKVRYRGVTKNDHWLHHRVAALNLRRL
ITIGLIRNGTTWAIA
AFHSTTLTYWRRRLVASDRPNRIFEAVKAVVTATGVLAKKTRRALDSTVLDDAVATQDTVTQLIAAIRRVGREVSGAGEVIAAVCSAHDYTDAGKPAIAW
NDKTARDQLVDGLVGDAHRLLGHLPEQDLGPRAAEAVALLALVAGQDVEPVEGSDGTDGHWQIAQKVAGDRVISTVDPEARHAHKTVHRRQDGFKAHIAV
EPDTGIITDCALTKASGPDNHEAVIGLALLADEHTPVRVLGDSAYGTGDARAALAELKHVAVIKPLPLRVPVPGGFTSDDFIIDFDARTVTCPAEHTVAI
GRTGGAGFEKHCRSCPLRQQCTTAKRGRKLTISEHEPHLRAARAVARTPEWQNEYRQHRPMVERSIAWLARGNRKVRYRGVTKNDHWLHHRVAALNLRRL
ITIGLIRNGTTWAIA
Blast result :
Comments
ISMgi2 is 48% aa similar to ISDce1.
References
1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Miller,C. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Miller,C. and Richardson,P. (2007) Direct submission GenBank.