ISCaa15

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010830 ND Candidatus Amoebophilus
Candidatus Amoebophilus asiaticus 5a2
DNA section
IS Length : 1434 bp

Ends


IR Length : 18

IRL : GGGGCTGTTGAAAAAGTAATTAAGAGTTTTAAATTAAGGTCACAGCCTAA
IRR : GGGGCTGTTGAAAAAGTATATGTTAAAAATATTCTATTAGGCATGTTAAG

Insertion site


Left flankDirect repeatRight flankDR Length
ACAAACAAACCACTGTCATTTAAG4
GCTATAAAAAGGTTGTTTTATTTT4
AAAATCTAATGGTTGTTTTT0

DNA sequence

GGGGCTGTTGAAAAAGTAATTAAGAGTTTTAAATTAAGGTCACAGCCTAATTTTAGCGTATATGTTATGGCAGAATAAAAATACTAATTATAGCACGAGC
CCAGTAGATGATAATCACTTACTTTCTAAAGTCAATAAGTATATAGATCTCTCCTTTACCCATCAGTTAGTTATAGCCTCTTATAATAGCAAGCATGGGC
GTCCCTCGATAGACCCAGAGATTTTCTTTAGGATGCTTATGATTAGCTATTTTTATAACATTAACTCTGATAGAAGAGTATGTCAAGAGATACGCTACAA
TGTAGCTTATAGATGGTTTTGTAAGCTTGGATTAGAAGACAAGGTGCCTGATCACTCTTACTTAAGTAGAACCAGAAACAGATTTGGCGAAAAAGTATTT
GAAGCGCTTTTTACTACTATACTCAATTTATGTCGAAAGCATGGCTTGTTGGAGAGTAATAGTGTGATGACAGATAGCACATTAATCAAAGCTAATGCAT
CACTAAATTCTTTAACTCCTATCGAAGCTAAAGCAGCAGCCTATGAGAAAGTAGCAAGAAAAGCTGCTATAAATAAGCTTGGGCCGCCTGCTAGTAGGAG
TATAAGCAACAGCACGCATATAAGCAAAACAGATAAAGATGCAAGCTTGGCTCAAAAGGAAGGGAGCCCCAGAGAGTTAAAATATAAAGTTCACAACTCA
ATTGATGCATTGAGTAGAGTAATAATTGATACAAAGGTTACAACAGGCAAGACCCATGATTCTCAAGTGTATATAGAGAGGCTAAATCACATTAGAGGAA
AGTATGCTCTAACTATAAAAGAAGCTATTGCAGATAGAGCTTACGGATCAAGAGCGATCATAGAGACTTTACAGAAAGAAGGTATAGTGACCTATATACC
ACTCTTTAGCACTAAAAGTGGTAGGTCTGTGCAAGAAGCTTATCAAGCTGGGTTTGTCTATCAAAAACAGAAGGATCGTTTTGTATGTCCTGAAGGCCAG
TATTTAAACCCTTATGGTTATATGGCTAATGAGAGTAAGTATTATAGGTCAAAATCATCCATTTGCGCTATGTGCAAGCAAAAAGATGCTTGTATTGCTT
CAGCTAAGAAAAGTAGGCCATTCACAAAATATCTTATCAGAAGCATCCATCAAGAGTTGTTTGATAAGACTCTTGAAGCTATGCAAGAACCAACATTCAT
TGGTAAGCTCAAAGAAAGGATGTGGAAAATAGAAGGTATTTTCGCTGAAGCTAAGCAATTACATGGGTTAGGTAAAGCTCGCTATAGGAGTTTGGAAAGA
GTGCAAATACAAGCATATATGATAGCTGTTGTACAAAATATTAAAAGAATAATTAAGCAGCTTTTTTATGTCTTTCTTCATTTCCTTAACATGCCTAATA
GAATATTTTTAACATATACTTTTTCAACAGCCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1373 bp457 aa621434+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLWQNKNTNYSTSPVDDNHLLSKVNKYIDLSFTHQLVIASYNSKHGRPSIDPEIFFRMLMISYFYNINSDRRVCQEIRYNVAYRWFCKLGLEDKVPDHSY
LSRTRNRFGEKVFEALFTTILNLCRKHGLLESNSVMTDSTLIKANASLNSLTPIEAKAAAYEKVARKAAINKLGPPASRSISNSTHISKTDKDASLAQKE
GSPRELKYKVHNSIDALSRVIIDTKVTTGKTHDSQVYIERLNHIRGKYALTIKEAIADRAYGSRAIIETLQKEGIVTYIPLFSTKSGRSVQEAYQAGFVY
QKQKDRFVCPEGQYLNPYGYMANESKYYRSKSSICAMCKQKDACIASAKKSRPFTKYLIRSIHQELFDKTLEAMQEPTFIGKLKERMWKIEGIFAEAKQL
HGLGKARYRSLERVQIQAYMIAVVQNIKRIIKQLFYVFLHFLNMPNRIFLTYTFSTA

 

Blast result :
Comments
ISCaa15 is 59% aa similar to ISLlo2.
ISCaa15 is present in three complete copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
There is no STOP codon, the transposase use the first STOP codon after the end of the IRR. The transposase in the database is cut at the end of the IS.
References
1] Schmitz-Esser S, Tischler P, Arnold R, Montanaro J, Wagner M, Rattei T, Horn M. (2010) J Bacteriol., 192(4):1045-1057.