ISCaa8
- Family IS5
- Group IS1031
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010830 | ND | Candidatus Amoebophilus | Candidatus Amoebophilus asiaticus 5a2 |
DNA section
IS Length : 891 bp
Ends
IR Length : 18/21
IRL : GAGCCTGTTCAAGATCTTTGTTCATTAAGTTAATATTTATTAGATTAGAG
IRR : GAGTCTGTTCATAATCTTTGTAGGCATATTCTTAGAAAAGCAAGCACCAC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TCTATTATTT | TA | AGAAATGCGT | 2 |
GACGGTTTGT | TA | AGATTCTAAA | 2 |
GAGTAAGTTC | TTA | CAATAAATCG | 3 |
AACAAGTTCT | TA | AGGCTTTTTG | 2 |
TTATTTCCCT | TA | AGGCATCAAA | 2 |
TGTAAATTCT | TA | AGGATAAGAA | 2 |
DNA sequence
GAGCCTGTTCAAGATCTTTGTTCATTAAGTTAATATTTATTAGATTAGAGAGGAAACTAGTCAATAACTTGATAAAAAAGGGACTTATCTATGACTCAAG
AGTATTCTACTAATATTAATAGAGAACAATTTGAAGTTATACGCCCTATTTTGGAAGCAGCCCGAAAAAAGACTAAACCTCGCCAGGTAGATTTATATGA
GGTATTCTCTGGATTACTGTATCTGCTCAAGACAGGCTGCCAATGGAGAATGTTGCCTAAAGATTTTCCTAAATGGCGTACGGTACATGCTTATTTTCAG
ATATGGAGTGAAAAGCAAGAAAATGGCATAAGCTTTCTAGAAGAAGTATTAAAAAAATATGGTTGCTCGTCTGAGGAAGCAAGCAGGTAAAGCAGCCCAA
ACGAGCCTGATTATAGTAGATGCGCAAAGCATAAAGAATACAGATACAGCCAAAGAGAAAGGCTATGATGGAGCTAAAAAGATAAACGGGATCAAGCGCC
ACATAGCCGTAGACAGCCAAGGCTTACCGCATGGGATATATGTTACACGGGCTAGTGAAACAGATAGAGATGGAGCAGTAACTATGCTTAAAGATCACAA
AGAGCAATTAGCTAAGGTAAAGAAGGTGCTCGTAGATGGAGGCTATAGTGGAGAAGTGTTTGCCAACCAAGTAAAAGAAGTCCTTCAGGGAGCGGAAGTA
GAAGTAGCTAAAAGGAGCGAATTGCATCGATTTAAGGTAATACCTAAGCGATGGATAGTGGAGCGAAGTTTTGGCTGGCTGGAAAAATGCCGACGCCTAT
GGAAAAACTGTGAGAGAAGATTAACTACAAGCTTACAAATGGTGGTGCTTGCTTTTCTAAGAATATGCCTACAAAGATTATGAACAGACTC
AGTATTCTACTAATATTAATAGAGAACAATTTGAAGTTATACGCCCTATTTTGGAAGCAGCCCGAAAAAAGACTAAACCTCGCCAGGTAGATTTATATGA
GGTATTCTCTGGATTACTGTATCTGCTCAAGACAGGCTGCCAATGGAGAATGTTGCCTAAAGATTTTCCTAAATGGCGTACGGTACATGCTTATTTTCAG
ATATGGAGTGAAAAGCAAGAAAATGGCATAAGCTTTCTAGAAGAAGTATTAAAAAAATATGGTTGCTCGTCTGAGGAAGCAAGCAGGTAAAGCAGCCCAA
ACGAGCCTGATTATAGTAGATGCGCAAAGCATAAAGAATACAGATACAGCCAAAGAGAAAGGCTATGATGGAGCTAAAAAGATAAACGGGATCAAGCGCC
ACATAGCCGTAGACAGCCAAGGCTTACCGCATGGGATATATGTTACACGGGCTAGTGAAACAGATAGAGATGGAGCAGTAACTATGCTTAAAGATCACAA
AGAGCAATTAGCTAAGGTAAAGAAGGTGCTCGTAGATGGAGGCTATAGTGGAGAAGTGTTTGCCAACCAAGTAAAAGAAGTCCTTCAGGGAGCGGAAGTA
GAAGTAGCTAAAAGGAGCGAATTGCATCGATTTAAGGTAATACCTAAGCGATGGATAGTGGAGCGAAGTTTTGGCTGGCTGGAAAAATGCCGACGCCTAT
GGAAAAACTGTGAGAGAAGATTAACTACAAGCTTACAAATGGTGGTGCTTGCTTTTCTAAGAATATGCCTACAAAGATTATGAACAGACTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
300 bp | 99 aa | 91 | 390 | + | No |
Description : First part of the transposase
ORF sequence :
MTQEYSTNINREQFEVIRPILEAARKKTKPRQVDLYEVFSGLLYLLKTGCQWRMLPKDFPKWRTVHAYFQIWSEKQENGISFLEEVLKKYGCSSEEASR
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
531 bp | 176 aa | 353 | 883 | + | No |
Description : Second part of the transposase
ORF sequence :
KNMVARLRKQAGKAAQTSLIIVDAQSIKNTDTAKEKGYDGAKKINGIKRHIAVDSQGLPHGIYVTRASETDRDGAVTMLKDHKEQLAKVKKVLVDGGYSG
EVFANQVKEVLQGAEVEVAKRSELHRFKVIPKRWIVERSFGWLEKCRRLWKNCERRLTTSLQMVVLAFLRICLQRL
EVFANQVKEVLQGAEVEVAKRSELHRFKVIPKRWIVERSFGWLEKCRRLWKNCERRLTTSLQMVVLAFLRICLQRL
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
793 bp | 263 aa | 91 | 883 | + | Yes |
Chemistry : DDE
ORF sequence :
MTQEYSTNINREQFEVIRPILEAARKKTKPRQVDLYEVFSGLLYLLKTGCQWRMLPKDFPKWRTVHAYFQIWSEKQENGISFLEEVLKNMVARLRKQAGK
AAQTSLIIVDAQSIKNTDTAKEKGYDGAKKINGIKRHIAVDSQGLPHGIYVTRASETDRDGAVTMLKDHKEQLAKVKKVLVDGGYSGEVFANQVKEVLQG
AEVEVAKRSELHRFKVIPKRWIVERSFGWLEKCRRLWKNCERRLTTSLQMVVLAFLRICLQRL
AAQTSLIIVDAQSIKNTDTAKEKGYDGAKKINGIKRHIAVDSQGLPHGIYVTRASETDRDGAVTMLKDHKEQLAKVKKVLVDGGYSGEVFANQVKEVLQG
AEVEVAKRSELHRFKVIPKRWIVERSFGWLEKCRRLWKNCERRLTTSLQMVVLAFLRICLQRL
Blast result :
Comments
ISCaa8 (orfAB) is 77% aa similar to ISCARN82.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
ISCaa8 is present in six copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
ISCaa8 is present in six copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
References
1] Schmitz-Esser S, Tischler P, Arnold R, Montanaro J, Wagner M, Rattei T, Horn M. (2010) J Bacteriol., 192(4):1045-1057.