ISGau2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP009153 | ND | Gemmatimonas aurantiaca | Gemmatimonas aurantiaca T-27 |
DNA section
IS Length : 1197 bp
Ends
IR Length : 30/39
IRL : GGGTAATCCCCCCGGAAATTAGGGGTCGTCAATAGTGGAATTTTCTCGTA
IRR : TGGTAATCCCCCCGTTTTTAGGGGGCGTCGTAAGTGGAGCTAGGCTACGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAGCTGACG | TTCCCATCCT | 0 |
DNA sequence
GGGTAATCCCCCCGGAAATTAGGGGTCGTCAATAGTGGAATTTTCTCGTACCGTTTTCGAGGAGGATTCCATGAAGAAGTCACGATTTACGGACAGCCAG
ATCATCGCGGTCTTGAAGGAGGCGGAGCTCGGGACACCGGTGCCGGAGCTCTGCCGATCGCACAGCATCAGCTCGGCGACGTTCAATAAATGGCGCGCCA
AATTCGGGGGCATGGACGTGTCCATGGTCACGCGGATGAAAGAGCTGGAGGAAGAGAATCGGCGACGCAAGAAGATGTATGCCGAGGCGCAGCTCAGCAC
CGATCTCCTGAAGGAAGCCCTCGCAAAAAAATGGTGAGGCCATCGCAACGTCGCGCGATGGCCCACCACGCCGTGAATGCGCAGCGCACCACCATCCGCC
ACGCGTGCGCGACGTTCGCGCTCAGCGAGACGTGCTATCGGTATCAGCGCAAACACGCCGACGAGAATGCCGTGATCGCCGACTGGCTGGTGCGGCTTAC
GACGGCGTATCGCACGTGGGGCTTCGGCTTGTGCTTCTTGCATCTGCGCAACGTGCGAGGGTTCACCTGGAACCACAAACGCGTGTATCGCATCTATCGT
TCACTCGAACTGAACCTCCGCATCAGCCCGCGGAAGCGGCTCATGCGCGCCACGCCGGCACCTTTGGCGGTGCCTGACGCGATCAATCAGACGTGGTCGA
TGGATTTCATGCACGACCAACTCCGCGATGGACGCACGTTTCGTCTCTTCAACGTGATCGACGACTTCAATCGCGAAGGCCTCCTGATCGAGGCCGATCT
GTCGCTTCCCGCGGAACGCGTCGTGCGAGCGCTCAATCAGCTCATCACGTGGCGTGGACAGCCGCAACGTATTCGCTGTGACAACGGCCCCGAGTACATC
GGGCACGTCATGCACGCGTGGGCCAAGCAACGCCACATCCAGCTCGATTTCATTCAGCCTGGGAAGCCCCAACAGAACGCGTACGTCGAACGCTACAATC
GCACGGTACGGCACGACTGGCTATCGCAGTGGCTCTTCGACAGCATCGCCGACGTGCAGGAATCCGCGACACGCTGGCTCTGGACATACAACAACGATCG
ACCGAATATGGCCCTGGGTGGCATCACCCCGAGCATGAAACTCACACGCGTAGCCTAGCTCCACTTACGACGCCCCCTAAAAACGGGGGGATTACCA
ATCATCGCGGTCTTGAAGGAGGCGGAGCTCGGGACACCGGTGCCGGAGCTCTGCCGATCGCACAGCATCAGCTCGGCGACGTTCAATAAATGGCGCGCCA
AATTCGGGGGCATGGACGTGTCCATGGTCACGCGGATGAAAGAGCTGGAGGAAGAGAATCGGCGACGCAAGAAGATGTATGCCGAGGCGCAGCTCAGCAC
CGATCTCCTGAAGGAAGCCCTCGCAAAAAAATGGTGAGGCCATCGCAACGTCGCGCGATGGCCCACCACGCCGTGAATGCGCAGCGCACCACCATCCGCC
ACGCGTGCGCGACGTTCGCGCTCAGCGAGACGTGCTATCGGTATCAGCGCAAACACGCCGACGAGAATGCCGTGATCGCCGACTGGCTGGTGCGGCTTAC
GACGGCGTATCGCACGTGGGGCTTCGGCTTGTGCTTCTTGCATCTGCGCAACGTGCGAGGGTTCACCTGGAACCACAAACGCGTGTATCGCATCTATCGT
TCACTCGAACTGAACCTCCGCATCAGCCCGCGGAAGCGGCTCATGCGCGCCACGCCGGCACCTTTGGCGGTGCCTGACGCGATCAATCAGACGTGGTCGA
TGGATTTCATGCACGACCAACTCCGCGATGGACGCACGTTTCGTCTCTTCAACGTGATCGACGACTTCAATCGCGAAGGCCTCCTGATCGAGGCCGATCT
GTCGCTTCCCGCGGAACGCGTCGTGCGAGCGCTCAATCAGCTCATCACGTGGCGTGGACAGCCGCAACGTATTCGCTGTGACAACGGCCCCGAGTACATC
GGGCACGTCATGCACGCGTGGGCCAAGCAACGCCACATCCAGCTCGATTTCATTCAGCCTGGGAAGCCCCAACAGAACGCGTACGTCGAACGCTACAATC
GCACGGTACGGCACGACTGGCTATCGCAGTGGCTCTTCGACAGCATCGCCGACGTGCAGGAATCCGCGACACGCTGGCTCTGGACATACAACAACGATCG
ACCGAATATGGCCCTGGGTGGCATCACCCCGAGCATGAAACTCACACGCGTAGCCTAGCTCCACTTACGACGCCCCCTAAAAACGGGGGGATTACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 71 | 337 | + | No |
Description : First part of the transposase
ORF sequence :
MKKSRFTDSQIIAVLKEAELGTPVPELCRSHSISSATFNKWRAKFGGMDVSMVTRMKELEEENRRRKKMYAEAQLSTDLLKEALAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 969 bp | 322 aa | 190 | 1158 | + | No |
Description : Second part of the transposase
ORF sequence :
MARQIRGHGRVHGHADERAGGRESATQEDVCRGAAQHRSPEGSPRKKMVRPSQRRAMAHHAVNAQRTTIRHACATFALSETCYRYQRKHADENAVIADWL
VRLTTAYRTWGFGLCFLHLRNVRGFTWNHKRVYRIYRSLELNLRISPRKRLMRATPAPLAVPDAINQTWSMDFMHDQLRDGRTFRLFNVIDDFNREGLLI
EADLSLPAERVVRALNQLITWRGQPQRIRCDNGPEYIGHVMHAWAKQRHIQLDFIQPGKPQQNAYVERYNRTVRHDWLSQWLFDSIADVQESATRWLWTY
NNDRPNMALGGITPSMKLTRVA
VRLTTAYRTWGFGLCFLHLRNVRGFTWNHKRVYRIYRSLELNLRISPRKRLMRATPAPLAVPDAINQTWSMDFMHDQLRDGRTFRLFNVIDDFNREGLLI
EADLSLPAERVVRALNQLITWRGQPQRIRCDNGPEYIGHVMHAWAKQRHIQLDFIQPGKPQQNAYVERYNRTVRHDWLSQWLFDSIADVQESATRWLWTY
NNDRPNMALGGITPSMKLTRVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 71 | 1158 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKSRFTDSQIIAVLKEAELGTPVPELCRSHSISSATFNKWRAKFGGMDVSMVTRMKELEEENRRRKKMYAEAQLSTDLLKEALAKKMVRPSQRRAMAHH
AVNAQRTTIRHACATFALSETCYRYQRKHADENAVIADWLVRLTTAYRTWGFGLCFLHLRNVRGFTWNHKRVYRIYRSLELNLRISPRKRLMRATPAPLA
VPDAINQTWSMDFMHDQLRDGRTFRLFNVIDDFNREGLLIEADLSLPAERVVRALNQLITWRGQPQRIRCDNGPEYIGHVMHAWAKQRHIQLDFIQPGKP
QQNAYVERYNRTVRHDWLSQWLFDSIADVQESATRWLWTYNNDRPNMALGGITPSMKLTRVA
AVNAQRTTIRHACATFALSETCYRYQRKHADENAVIADWLVRLTTAYRTWGFGLCFLHLRNVRGFTWNHKRVYRIYRSLELNLRISPRKRLMRATPAPLA
VPDAINQTWSMDFMHDQLRDGRTFRLFNVIDDFNREGLLIEADLSLPAERVVRALNQLITWRGQPQRIRCDNGPEYIGHVMHAWAKQRHIQLDFIQPGKP
QQNAYVERYNRTVRHDWLSQWLFDSIADVQESATRWLWTYNNDRPNMALGGITPSMKLTRVA
Blast result :
Comments
ISGau2 is 86% aa similar to ISXac2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Ichikawa, N. (2010) Direct submission
2] Oguchi,A., Ankai,A., Yashiro,I., Takahashi,M., Terui,Y., Fukui,S., Yokoyama,H., Ichikawa,N., Takasaki,K., Miura,H., Matsushita,S., Watanabe,Y., Sekiguchi,Y., Nakamura,K., Tanikawa,S., Hanada,S., Kamagata,Y., Fujita,N. and Kikuchi,H. (2006) Direct submission Genbank
2] Oguchi,A., Ankai,A., Yashiro,I., Takahashi,M., Terui,Y., Fukui,S., Yokoyama,H., Ichikawa,N., Takasaki,K., Miura,H., Matsushita,S., Watanabe,Y., Sekiguchi,Y., Nakamura,K., Tanikawa,S., Hanada,S., Kamagata,Y., Fujita,N. and Kikuchi,H. (2006) Direct submission Genbank