ISPso2
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY237732 | Y | Paracoccus solventivorans | Paracoccus solventivorans DSM 11592 |
DNA section
IS Length : 832 bp
Ends
IR Length : 13/14
IRL : GGGTCAGGATGCATTGATTTCTGTTGCGCTGCGTGATTCACGCCTCCGAA
IRR : GGGTCAGGATTCATAACCAATAGATGACGAGAGCCGCGAGGGCGATGGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGTTGTGCT | TA | CCCATCTCTC | 2 |
DNA sequence
GGGTCAGGATGCATTGATTTCTGTTGCGCTGCGTGATTCACGCCTCCGAAAACGGAGCGGTGACATGAGCGATCTCTTCTGGCTGACCGACGCGCAGATG
GCGCGCCTGGCTCCCTTCTTTCCCAAGTCGCACGGGAAGCCCCGGGTTGATGACAGACGGGTGCTGAGCGGGATTATTTTCATCAATCGCAATGGCTTGC
GTTGGCGCGATGCGCCCGCCGCGTATGGCCCACATAAGACGCTCTACAGCCGGTGGAAGCGTTGGAGCGAAAAGGGCATCTTCGCGCGGATGATGGCCGG
GCTGGCGGCGGAACACGGCGAGAAAACGACCGTGATGATCGACGCAACATACCTCAAGGCCCATCGAACGGCGACCAGCATGGCCGCCAAAAAGGGGGGC
GTGGTCGCCTGATCGGTCGAACCAAAGGCGGTATGAACACCAAGCTGCACGCCATCTGCGACAGTCAGGGGCGACCGATCGACCTGTTCGTCACCGCTGG
ACAGGTCAGCGATTATATCGGCGCTCGGGCCCTGCTGAGTGGCCTGCCAAACGTCAAATGGCTACTCGGAGATCGTGGCTATGACGCTGACTGGTTCAGA
GAAGCGTTGCAGGACAAGGGGATACGTGCCTGCATCCCAGGCCGGAAGAAACGCAAGACGGCGATCAAATACGATAAGCGGAGATACAAGCGGCGTAACC
GCATCGAGATCATGTTCGGCAGGCTCAAGGACTGGAGGCGCGTCGCGACCCGCTATGACCGATGCCCCAAGGTCTTCCTCTCAGCCATCGCCCTCGCGGC
TCTCGTCATCTATTGGTTATGAATCCTGACCC
GCGCGCCTGGCTCCCTTCTTTCCCAAGTCGCACGGGAAGCCCCGGGTTGATGACAGACGGGTGCTGAGCGGGATTATTTTCATCAATCGCAATGGCTTGC
GTTGGCGCGATGCGCCCGCCGCGTATGGCCCACATAAGACGCTCTACAGCCGGTGGAAGCGTTGGAGCGAAAAGGGCATCTTCGCGCGGATGATGGCCGG
GCTGGCGGCGGAACACGGCGAGAAAACGACCGTGATGATCGACGCAACATACCTCAAGGCCCATCGAACGGCGACCAGCATGGCCGCCAAAAAGGGGGGC
GTGGTCGCCTGATCGGTCGAACCAAAGGCGGTATGAACACCAAGCTGCACGCCATCTGCGACAGTCAGGGGCGACCGATCGACCTGTTCGTCACCGCTGG
ACAGGTCAGCGATTATATCGGCGCTCGGGCCCTGCTGAGTGGCCTGCCAAACGTCAAATGGCTACTCGGAGATCGTGGCTATGACGCTGACTGGTTCAGA
GAAGCGTTGCAGGACAAGGGGATACGTGCCTGCATCCCAGGCCGGAAGAAACGCAAGACGGCGATCAAATACGATAAGCGGAGATACAAGCGGCGTAACC
GCATCGAGATCATGTTCGGCAGGCTCAAGGACTGGAGGCGCGTCGCGACCCGCTATGACCGATGCCCCAAGGTCTTCCTCTCAGCCATCGCCCTCGCGGC
TCTCGTCATCTATTGGTTATGAATCCTGACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 65 | 412 | + | No |
Description :
ORF sequence :
MSDLFWLTDAQMARLAPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWRDAPAAYGPHKTLYSRWKRWSEKGIFARMMAGLAAEHGEKTTVMIDATYLKAH
RTATSMAAKKGGVVA
RTATSMAAKKGGVVA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 570 bp | 189 aa | 253 | 822 | + | No |
Description :
ORF sequence :
MEALERKGHLRADDGRAGGGTRRENDRDDRRNIPQGPSNGDQHGRQKGGRGRLIGRTKGGMNTKLHAICDSQGRPIDLFVTAGQVSDYIGARALLSGLPN
VKWLLGDRGYDADWFREALQDKGIRACIPGRKKRKTAIKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAALVIYWL
VKWLLGDRGYDADWFREALQDKGIRACIPGRKKRKTAIKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAALVIYWL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 758 bp | 252 aa | 65 | 822 | + | Yes |
Description :
ORF sequence :
MSDLFWLTDAQMARLAPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWRDAPAAYGPHKTLYSRWKRWSEKGIFARMMAGLAAEHGEKTTVMIDATYLKAH
RTATSMAAKKGGRGRLIGRTKGGMNTKLHAICDSQGRPIDLFVTAGQVSDYIGARALLSGLPNVKWLLGDRGYDADWFREALQDKGIRACIPGRKKRKTA
IKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAALVIYWL
RTATSMAAKKGGRGRLIGRTKGGMNTKLHAICDSQGRPIDLFVTAGQVSDYIGARALLSGLPNVKWLLGDRGYDADWFREALQDKGIRACIPGRKKRKTA
IKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAALVIYWL
Blast result :
Comments
ISPso2 has been identified by its transposition into entrapment vector pMEC1 (cI-tetA cassette).
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift. At position 389, ISPso2 contains a sequence 5'-AAAAAG-3' that might function as a -1 translational frameshifting site.
ISPso2 is 96% aa similar to IS1248A.
As judged from DNA hybridization analysis ISPso2 is present in multiple copies in the genome of Paracoccus solventivorans DSM 11592.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift. At position 389, ISPso2 contains a sequence 5'-AAAAAG-3' that might function as a -1 translational frameshifting site.
ISPso2 is 96% aa similar to IS1248A.
As judged from DNA hybridization analysis ISPso2 is present in multiple copies in the genome of Paracoccus solventivorans DSM 11592.
References
1] Bartosik,D., Szymanik,M., and Baj,J. (2003) Appl. Environ. Microbiol. 69, 7002-7008.
2] Bartosik,D. (2010) Direct submission GenBank.
2] Bartosik,D. (2010) Direct submission GenBank.