ISPme2
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
EU016112 | Y | Paracoccus methylutens | Paracoccus methylutens DM12 |
DNA section
IS Length : 851 bp
Ends
IR Length : 15/18
IRL : GGGCGTGTTGACAAAAGGGATTCACCGGGCGCGGTGATCGTGATTCAAGC
IRR : GGTCATGTTGACAAATGGCGGAGCCAGAGGCGGATCGACGTGATGTCGAT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
AACGTCGCCT | TA | AAGCAATTTA | 2 |
DNA sequence
GGGCGTGTTGACAAAAGGGATTCACCGGGCGCGGTGATCGTGATTCAAGCTGGTATCTGCAATGGAGACCAGCGTGGCACGAAACCTGATGTCGAACGAC
GAGTGGGCGTTCTTTGAACGCTTCATCCTGGCTGTCCGTTCTCCGAACGGCCGCAAGCCCACGAACCATCGGCTTGTTCTGGATGGGATTTTCTGGATCG
CCCGAACGGGGTCGCCCTGGCGCGACCTGCCGGAAGAGTTCGGCAAATGGTCGTCTGTCTATCGGCAGTTCCGGCGCTGGACGTTGGCGGGGCTGTGGGA
ACAGATCCTGGAGGCGCTGAACGAGAGCCGGATCGTGCCGGATGCGCTGCAGATGATCGACAGCACCGTGGTTCGCGCTCATCATCAGGCAGCGGGCGCA
AAAGGGGGACTCCGCGCCAAGGTTTCGGCCGCTCGCGAGGTGGCTTCACGACCAAGATCCATCTGCGGGTCAATGCGGCAGGCCTGCCCATGAGAACCGA
GATCACGGCCGGGCAGACATCGGACTACCTCGGCTTCGATCTGGTCATGGCTGACAACCTGCCCGAGCCAAGCGTTCTGCTCGCAGACCGCGGCTACGAC
TCCGACAACATCCGCAAGACCATGGAGGTGCGCGACGTGGTGCCGGTCATCCCCATGCGCAAAACCCGGAAGCTACGGGTCGCCGTTGACCGCAGGCTCT
ACCGCCTGCGCAATCTCGTTGAGCGGTGCTTCAACAAGCTCAAGAATGCCCGTCGCGTCGCCACCCGCTACGACAAGACCGCCGAAAGCTTCCTGGGCTT
CATCGACATCACGTCGATCCGCCTCTGGCTCCGCCATTTGTCAACATGACC
GAGTGGGCGTTCTTTGAACGCTTCATCCTGGCTGTCCGTTCTCCGAACGGCCGCAAGCCCACGAACCATCGGCTTGTTCTGGATGGGATTTTCTGGATCG
CCCGAACGGGGTCGCCCTGGCGCGACCTGCCGGAAGAGTTCGGCAAATGGTCGTCTGTCTATCGGCAGTTCCGGCGCTGGACGTTGGCGGGGCTGTGGGA
ACAGATCCTGGAGGCGCTGAACGAGAGCCGGATCGTGCCGGATGCGCTGCAGATGATCGACAGCACCGTGGTTCGCGCTCATCATCAGGCAGCGGGCGCA
AAAGGGGGACTCCGCGCCAAGGTTTCGGCCGCTCGCGAGGTGGCTTCACGACCAAGATCCATCTGCGGGTCAATGCGGCAGGCCTGCCCATGAGAACCGA
GATCACGGCCGGGCAGACATCGGACTACCTCGGCTTCGATCTGGTCATGGCTGACAACCTGCCCGAGCCAAGCGTTCTGCTCGCAGACCGCGGCTACGAC
TCCGACAACATCCGCAAGACCATGGAGGTGCGCGACGTGGTGCCGGTCATCCCCATGCGCAAAACCCGGAAGCTACGGGTCGCCGTTGACCGCAGGCTCT
ACCGCCTGCGCAATCTCGTTGAGCGGTGCTTCAACAAGCTCAAGAATGCCCGTCGCGTCGCCACCCGCTACGACAAGACCGCCGAAAGCTTCCTGGGCTT
CATCGACATCACGTCGATCCGCCTCTGGCTCCGCCATTTGTCAACATGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
432 bp | 143 aa | 62 | 493 | + | No |
Description : First part of the transposase
ORF sequence :
METSVARNLMSNDEWAFFERFILAVRSPNGRKPTNHRLVLDGIFWIARTGSPWRDLPEEFGKWSSVYRQFRRWTLAGLWEQILEALNESRIVPDALQMID
STVVRAHHQAAGAKGGLRAKVSAAREVASRPRSICGSMRQACP
STVVRAHHQAAGAKGGLRAKVSAAREVASRPRSICGSMRQACP
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
603 bp | 200 aa | 247 | 849 | + | No |
Description : Second part of the transposase
ORF sequence :
MVVCLSAVPALDVGGAVGTDPGGAEREPDRAGCAADDRQHRGSRSSSGSGRKRGTPRQGFGRSRGGFTTKIHLRVNAAGLPMRTEITAGQTSDYLGFDLV
MADNLPEPSVLLADRGYDSDNIRKTMEVRDVVPVIPMRKTRKLRVAVDRRLYRLRNLVERCFNKLKNARRVATRYDKTAESFLGFIDITSIRLWLRHLST
MADNLPEPSVLLADRGYDSDNIRKTMEVRDVVPVIPMRKTRKLRVAVDRRLYRLRNLVERCFNKLKNARRVATRYDKTAESFLGFIDITSIRLWLRHLST
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
788 bp | 262 aa | 62 | 849 | + | Yes |
Chemistry : DDE
ORF sequence :
METSVARNLMSNDEWAFFERFILAVRSPNGRKPTNHRLVLDGIFWIARTGSPWRDLPEEFGKWSSVYRQFRRWTLAGLWEQILEALNESRIVPDALQMID
STVVRAHHQAAGAKRGTPRQGFGRSRGGFTTKIHLRVNAAGLPMRTEITAGQTSDYLGFDLVMADNLPEPSVLLADRGYDSDNIRKTMEVRDVVPVIPMR
KTRKLRVAVDRRLYRLRNLVERCFNKLKNARRVATRYDKTAESFLGFIDITSIRLWLRHLST
STVVRAHHQAAGAKRGTPRQGFGRSRGGFTTKIHLRVNAAGLPMRTEITAGQTSDYLGFDLVMADNLPEPSVLLADRGYDSDNIRKTMEVRDVVPVIPMR
KTRKLRVAVDRRLYRLRNLVERCFNKLKNARRVATRYDKTAESFLGFIDITSIRLWLRHLST
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISPme2 is 88% aa similar to ISPaes1.
ISPme2 has been identified by its transposition into entrapment vector pMEC1 (cI-tetA cassette).
ISPme2 is 88% aa similar to ISPaes1.
ISPme2 has been identified by its transposition into entrapment vector pMEC1 (cI-tetA cassette).
References
1] Bartosik, D., M. Putyrski, L. Dziewit, E. Malewska, M. Szymanik, E. Jagiello, J. Lukasik and J. Baj.(2008). J Bacteriol 190: 3306-3313.
2] Dziewit, L. and Bartosik, D. (2010) Direct submission
3] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277
2] Dziewit, L. and Bartosik, D. (2010) Direct submission
3] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277