ISPso1
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY237733 | Y | Paracoccus solventivorans | Paracoccus solventivorans DSM 11592 |
DNA section
IS Length : 1400 bp
Ends
IR Length : 27/33
IRL : GGCAGTGTCCCGGGTGGTGGTGTAGGAGGCCGCGCGCGGAGCCTCCGGCA
IRR : GATAGTGTCCCGGGGGATGGTGTAAGAGCGCCGACCCTCGGAGAGGTCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCTCTCCGC | ATCACCTT | TGGTAAAGGT | 8 |
DNA sequence
GGCAGTGTCCCGGGTGGTGGTGTAGGAGGCCGCGCGCGGAGCCTCCGGCATAGCGCAGCGCGCGGCCGCGGGTGACGCTGGCGGTCATCGCCGATCTTCG
GAGAAGGTTCGGGTTGCGAGACCTGGAACCGACAACGGAGAGGACGATGACCGAAGAGAGAATGGCGCTGATCGAGCTGGTTGAGAAGAAGGCCGATGGC
GACCTCGTGCGCGAGATGCTGGCCTTCGCAGCTGAGCGCATCATGGAGACCGAGGTGGAAGCACGGACGGGCGCGGCCAAGGGCGCACGTACGCCGATGC
GGGAGGTGCAGCGCAACGGATACCGCGACCGCGACTGGGATACTCGGGCCGGTCGCATCGCGCTGGAGATCCCGAAGCTGCGCCGGGGGAGCTACTTCCC
CAGCTTCCTGGAACCGCGAAGGACCGCCGAGAAGGCCCTCGTGGCCGTGATCCAGGAGGCCTACGTCCACGGCATCTCGACGCGGGCCGTCGATGATCTG
GTGAAGGCCATGGGCGCGGGCGGCATGTCGAAGAGCCAGGTCAGCCGGCTCTGCGCTGAGATCGACGAGCGGGTGAACGCATTCCTGTCGCGGCCCTTGG
AAGGCGCATGGCCCTATCTGTGGCTGGACGCGACCTACGTGAAGGTGCGCGAGGGCGGGCGCATCGTCAGCCGCGCCGTGATAATCGCGGTGGCCGTGAA
CGACGACGGCAAACGCGAGGTGCTGGGCGTCGCCACCGGCCCCTCCGAAGCCGAGACGTTCTGGACCGAGTTCCTGCGTTCGTTGGCTGATCGCGGTCTG
CGGGGCGTGAAGCTGGTGATCGCTGACGACCACAAGGGCCTCCGGGCCGCAGCGCGCCGGGTCTTCAACGCCACCCACCAGAGGTGCCGCATTCACTGGA
TGCGGAACGCCCTGGCCCATGCCCCGGCCAAGCAGCGCACCGCGGTCGCGGCGATGCTAAAGACGATCTTCGCGCAGGAGACCAAGGTTGAGGCCGAGAC
ACAGTGGGATATCGTTGCGGACGCGCTCCGCGAGAAGCAGCCCAAACTCGGCGCGCTGATGGACAGCTCTCGCGACGACGTGCTGGCGTACATGACCTTC
CCCCGCGAACATTGGGCCCAGATCGCCTCGACGAACCCACTGGAGCGGGTGAACCGCGAGGTGAAGCGGCGCGCCGACGTCATCGGCATCTTCCCGAACG
ACGAGGCCATCGTCCGCCTGGTTGGCGCGCTGATGCTGGAGACCAACGACGAGTGGGCGGTCGCGCGGCGCTACATGAGCCTTGAAACGCTCGCCCGCCT
CACCGACAATCCCACCGTCAGGCTGTCCGCTGTGGCGACCTGATCAAGCCCTGACCTCTCCGAGGGTCGGCGCTCTTACACCATCCCCCGGGACACTATC
GAGAAGGTTCGGGTTGCGAGACCTGGAACCGACAACGGAGAGGACGATGACCGAAGAGAGAATGGCGCTGATCGAGCTGGTTGAGAAGAAGGCCGATGGC
GACCTCGTGCGCGAGATGCTGGCCTTCGCAGCTGAGCGCATCATGGAGACCGAGGTGGAAGCACGGACGGGCGCGGCCAAGGGCGCACGTACGCCGATGC
GGGAGGTGCAGCGCAACGGATACCGCGACCGCGACTGGGATACTCGGGCCGGTCGCATCGCGCTGGAGATCCCGAAGCTGCGCCGGGGGAGCTACTTCCC
CAGCTTCCTGGAACCGCGAAGGACCGCCGAGAAGGCCCTCGTGGCCGTGATCCAGGAGGCCTACGTCCACGGCATCTCGACGCGGGCCGTCGATGATCTG
GTGAAGGCCATGGGCGCGGGCGGCATGTCGAAGAGCCAGGTCAGCCGGCTCTGCGCTGAGATCGACGAGCGGGTGAACGCATTCCTGTCGCGGCCCTTGG
AAGGCGCATGGCCCTATCTGTGGCTGGACGCGACCTACGTGAAGGTGCGCGAGGGCGGGCGCATCGTCAGCCGCGCCGTGATAATCGCGGTGGCCGTGAA
CGACGACGGCAAACGCGAGGTGCTGGGCGTCGCCACCGGCCCCTCCGAAGCCGAGACGTTCTGGACCGAGTTCCTGCGTTCGTTGGCTGATCGCGGTCTG
CGGGGCGTGAAGCTGGTGATCGCTGACGACCACAAGGGCCTCCGGGCCGCAGCGCGCCGGGTCTTCAACGCCACCCACCAGAGGTGCCGCATTCACTGGA
TGCGGAACGCCCTGGCCCATGCCCCGGCCAAGCAGCGCACCGCGGTCGCGGCGATGCTAAAGACGATCTTCGCGCAGGAGACCAAGGTTGAGGCCGAGAC
ACAGTGGGATATCGTTGCGGACGCGCTCCGCGAGAAGCAGCCCAAACTCGGCGCGCTGATGGACAGCTCTCGCGACGACGTGCTGGCGTACATGACCTTC
CCCCGCGAACATTGGGCCCAGATCGCCTCGACGAACCCACTGGAGCGGGTGAACCGCGAGGTGAAGCGGCGCGCCGACGTCATCGGCATCTTCCCGAACG
ACGAGGCCATCGTCCGCCTGGTTGGCGCGCTGATGCTGGAGACCAACGACGAGTGGGCGGTCGCGCGGCGCTACATGAGCCTTGAAACGCTCGCCCGCCT
CACCGACAATCCCACCGTCAGGCTGTCCGCTGTGGCGACCTGATCAAGCCCTGACCTCTCCGAGGGTCGGCGCTCTTACACCATCCCCCGGGACACTATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1197 bp | 398 aa | 147 | 1343 | + | No |
Chemistry : DDE
ORF sequence :
MTEERMALIELVEKKADGDLVREMLAFAAERIMETEVEARTGAAKGARTPMREVQRNGYRDRDWDTRAGRIALEIPKLRRGSYFPSFLEPRRTAEKALVA
VIQEAYVHGISTRAVDDLVKAMGAGGMSKSQVSRLCAEIDERVNAFLSRPLEGAWPYLWLDATYVKVREGGRIVSRAVIIAVAVNDDGKREVLGVATGPS
EAETFWTEFLRSLADRGLRGVKLVIADDHKGLRAAARRVFNATHQRCRIHWMRNALAHAPAKQRTAVAAMLKTIFAQETKVEAETQWDIVADALREKQPK
LGALMDSSRDDVLAYMTFPREHWAQIASTNPLERVNREVKRRADVIGIFPNDEAIVRLVGALMLETNDEWAVARRYMSLETLARLTDNPTVRLSAVAT
VIQEAYVHGISTRAVDDLVKAMGAGGMSKSQVSRLCAEIDERVNAFLSRPLEGAWPYLWLDATYVKVREGGRIVSRAVIIAVAVNDDGKREVLGVATGPS
EAETFWTEFLRSLADRGLRGVKLVIADDHKGLRAAARRVFNATHQRCRIHWMRNALAHAPAKQRTAVAAMLKTIFAQETKVEAETQWDIVADALREKQPK
LGALMDSSRDDVLAYMTFPREHWAQIASTNPLERVNREVKRRADVIGIFPNDEAIVRLVGALMLETNDEWAVARRYMSLETLARLTDNPTVRLSAVAT
Blast result :
Comments
ISPso1 has been identified by its transposition into entrapment vector pMEC1 (cI-tetA cassette). As judged from DNA hybridization analysis ISPso1 is present in 2 copies in the genome of Paracoccus solventivorans DSM 11592.
ISPso1 is 85% aa similar to ISGdi8.
ISPso1 is 85% aa similar to ISGdi8.
References
1]Bartosik,D., Szymanik,M., and Baj,J. (2003) Appl. Environ. Microbiol. 69, 7002-7008.
2]Bartosik,D. (2010) Direct submission.
2]Bartosik,D. (2010) Direct submission.