ISPaes2

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
GU826198 Y Paracoccus aestuarii
Paracoccus aestuarii DSM 19484
DNA section
IS Length : 841 bp

Ends


IR Length : 18/21

IRL : GGCTCCAGACCCATTGATCTTACGGTCTTTGCATGATTCATGCTCCTCAA
IRR : GGCTCCAGACTCATTAAGTTTCACAGCCAGAACAAGACGGTTGCGGCGAG

Insertion site


Left flankDirect repeatRight flankDR Length
GCATTACGTCTAACGATATTTA2

DNA sequence

GGCTCCAGACCCATTGATCTTACGGTCTTTGCATGATTCATGCTCCTCAAGGAGACCTGATCGATGAGTAATCTTTACTGGCTGAGCGACGCCCAAATGG
AGCGTCTGAAACCGTTCTTTCCCAAGAGCCATGGCAAGCCCCGGGTCGATGATCGTCGCGTCCTCAGCGGCATAATTTTCATCAATCGTAATGGGTTGCG
GTGGTGTGACGCGCCGAAGGAATACGGCCCGGCCAAAACCCTCTACAACCGCTGGAAGCGCTGGAGCGATAACGGGGTTTTTGCCCGGATCATTGTCGGC
CTTGCCGCCGAGAGCGCCGAGCACAAGACGATCATGATTGACGCGACCTATCTCAAGGCACACCGCACGGCCTCGAGCCTTGGGGTGAAAAAGGGGGGCG
CGGGCGCCAGATCGGGCGCACCAAAGGCGGTATGAACACCAAGTTGCACGCTGTCGCCGATGCGAAGGGGCGGCCGATCGGGTTCTTCATGTCGGCCGGC
CAGGTTAGCGATTACACCGGCGCGGCGGCGCTGCTGGGCAGCCTGCCGAAGGCTGAGTGGCTTCTGGCCGACAGGGGCTACGACGCTGATTGGTTCAGAG
ACGCTTTGAAAGACAAGGGGATAAAGGTTTGCATCCCCGGACGGAGGTCCCGCAAGAAGGTCGTCAAATACGACAAGCGGCGTTACAAAAGGCGTAACCG
CATCGAAATCATGTTCGGACGTCTGAAGGACTGGAGGCGCGTCGCAACCCGTTATGACCGCTGCCCGGAAACGTTCTTCTCAGCGATCATGCTCGCCGCA
ACCGTCTTGTTCTGGCTGTGAAACTTAATGAGTCTGGAGCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
372 bp123 aa64435+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSNLYWLSDAQMERLKPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIIVGLAAESAEHKTIMIDATYLKAH
RTASSLGVKKGGAGARSGAPKAV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
480 bp159 aa342821+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RDLSQGTPHGLEPWGEKGGRGRQIGRTKGGMNTKLHAVADAKGRPIGFFMSAGQVSDYTGAAALLGSLPKAEWLLADRGYDADWFRDALKDKGIKVCIPG
RRSRKKVVKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPETFFSAIMLAATVLFWL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
758 bp252 aa64821+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSNLYWLSDAQMERLKPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIIVGLAAESAEHKTIMIDATYLKAH
RTASSLGVKKGGRGRQIGRTKGGMNTKLHAVADAKGRPIGFFMSAGQVSDYTGAAALLGSLPKAEWLLADRGYDADWFRDALKDKGIKVCIPGRRSRKKV
VKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPETFFSAIMLAATVLFWL

 

Blast result :
Comments
References
1] Dziewit, L. and Bartosik, D. (2010) Direct submission
2] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277