ISPbe1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| GQ871936 | Y | Paracoccus bengalensis | Paracoccus bengalensis DSM 17099 |
DNA section
IS Length : 1200 bp
Ends
IR Length : 12
IRL : TGGTAATCCTCCCCGAAAGTAAGGGGCTGCATAAGTAGAATTTTCTCGGC
IRR : TGGTAATCCCCCCATTTTTAGCGGGGTGCATCCGTAGAATTCACGCGGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAAAAGAAA | AA | TGAACTTGGCT | 2 |
DNA sequence
TGGTAATCCTCCCCGAAAGTAAGGGGCTGCATAAGTAGAATTTTCTCGGCAAGATGAACGAGGAGATTCTGAATGAAGACAAGCAGATACACTGAAGCGC
AGATCCTTTCGATCCTGCGTCAGGCCGAAGGCGGGATGCCGGTGGCCGAGCTGTGCCGCGAGCATGGCATGAGCAGCGCGTCGTTTTACAAGTGGCGGGC
GAAGTATGGGGGCATGGACGCGTCCCTGATCAGCCAGATGAAGGCTATGGAAGAGGAGAACCGGCGGCTGAAGCGGATGTACGCTGATCTCAGCATGCAG
GCCGATTTGCTGAAGGAGGCTCTCGGAAAAAAATGACGCGGCCATCTCAACGCCGCGAGATGGCCGAGAAAGCGGTGGCGCGACACGGGGTCAGCATTGC
GCTGGCTTGCCGGGCCTTCGGGGTGAGCGAGACCTGTTATCGCTACAGCCCGAAGCTGAAGGACGAGAACGAGGAGATCGCCGATCTGCTGGTCGGGCTG
ACGGACGCCCGCAAGACCTGGGGGTTTGGCCTGTGTTTCCTGCATTTGCGCAACGTGAAGGGGCATCCATGGAACCACAAACGTGTCTATCGCATCTATT
GCGCGCTGGAACTGAACCTGCGGATCAAGCCCCGCAAACGGCTGAAGCGTGAGAAGCCCGAGGCTCTGGCCGTGCCGGATGCGCCGAACCTGACCTGGTC
CATGGACTTCATGGCCGATCGTCTGGGGGATGGCCGCCAGTTCCGGCTGTTGAACGTGCTGGACGACTTCAACCGGGAGGGCTTGGGCATCGAGGTCGAC
TTTTCACTGCCAGCGGAAAGGGTCATCAGAAGCCTCGACAGGATCATTGAATGGCGCGGCAAACCCGGCACCATTCGGGTCGACAATGGCCCGGAATACA
TCAGCGGCAAGCTGATGGAATGGGCCGAAAAGCGGAAGATCACCATCCAGCACATCCAACCCGGACAGCCCCAGCAGAACGCCTATATCGAGCGCTACAA
CCGCACCGTACGGCATGAATGGCTCGATCAATACATCATCGAAACCATCGAGGAGGCTCAGGACTTCGCCACGCAATGGCTCTGGACTTACAACAACGAC
CGCCCGAACATGGGCATCGGCGGCATCACACCCGCACAGAAACTGAAGATGGCCGCGTGAATTCTACGGATGCACCCCGCTAAAAATGGGGGGATTACCA
AGATCCTTTCGATCCTGCGTCAGGCCGAAGGCGGGATGCCGGTGGCCGAGCTGTGCCGCGAGCATGGCATGAGCAGCGCGTCGTTTTACAAGTGGCGGGC
GAAGTATGGGGGCATGGACGCGTCCCTGATCAGCCAGATGAAGGCTATGGAAGAGGAGAACCGGCGGCTGAAGCGGATGTACGCTGATCTCAGCATGCAG
GCCGATTTGCTGAAGGAGGCTCTCGGAAAAAAATGACGCGGCCATCTCAACGCCGCGAGATGGCCGAGAAAGCGGTGGCGCGACACGGGGTCAGCATTGC
GCTGGCTTGCCGGGCCTTCGGGGTGAGCGAGACCTGTTATCGCTACAGCCCGAAGCTGAAGGACGAGAACGAGGAGATCGCCGATCTGCTGGTCGGGCTG
ACGGACGCCCGCAAGACCTGGGGGTTTGGCCTGTGTTTCCTGCATTTGCGCAACGTGAAGGGGCATCCATGGAACCACAAACGTGTCTATCGCATCTATT
GCGCGCTGGAACTGAACCTGCGGATCAAGCCCCGCAAACGGCTGAAGCGTGAGAAGCCCGAGGCTCTGGCCGTGCCGGATGCGCCGAACCTGACCTGGTC
CATGGACTTCATGGCCGATCGTCTGGGGGATGGCCGCCAGTTCCGGCTGTTGAACGTGCTGGACGACTTCAACCGGGAGGGCTTGGGCATCGAGGTCGAC
TTTTCACTGCCAGCGGAAAGGGTCATCAGAAGCCTCGACAGGATCATTGAATGGCGCGGCAAACCCGGCACCATTCGGGTCGACAATGGCCCGGAATACA
TCAGCGGCAAGCTGATGGAATGGGCCGAAAAGCGGAAGATCACCATCCAGCACATCCAACCCGGACAGCCCCAGCAGAACGCCTATATCGAGCGCTACAA
CCGCACCGTACGGCATGAATGGCTCGATCAATACATCATCGAAACCATCGAGGAGGCTCAGGACTTCGCCACGCAATGGCTCTGGACTTACAACAACGAC
CGCCCGAACATGGGCATCGGCGGCATCACACCCGCACAGAAACTGAAGATGGCCGCGTGAATTCTACGGATGCACCCCGCTAAAAATGGGGGGATTACCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 87 aa | 73 | 336 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSRYTEAQILSILRQAEGGMPVAELCREHGMSSASFYKWRAKYGGMDASLISQMKAMEEENRRLKRMYADLSMQADLLKEALGKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 873 bp | 290 aa | 288 | 1160 | + | No |
Description : Second part of the transposase
ORF sequence :
SQHAGRFAEGGSRKKMTRPSQRREMAEKAVARHGVSIALACRAFGVSETCYRYSPKLKDENEEIADLLVGLTDARKTWGFGLCFLHLRNVKGHPWNHKRV
YRIYCALELNLRIKPRKRLKREKPEALAVPDAPNLTWSMDFMADRLGDGRQFRLLNVLDDFNREGLGIEVDFSLPAERVIRSLDRIIEWRGKPGTIRVDN
GPEYISGKLMEWAEKRKITIQHIQPGQPQQNAYIERYNRTVRHEWLDQYIIETIEEAQDFATQWLWTYNNDRPNMGIGGITPAQKLKMAA
YRIYCALELNLRIKPRKRLKREKPEALAVPDAPNLTWSMDFMADRLGDGRQFRLLNVLDDFNREGLGIEVDFSLPAERVIRSLDRIIEWRGKPGTIRVDN
GPEYISGKLMEWAEKRKITIQHIQPGQPQQNAYIERYNRTVRHEWLDQYIIETIEEAQDFATQWLWTYNNDRPNMGIGGITPAQKLKMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 73 | 1160 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSRYTEAQILSILRQAEGGMPVAELCREHGMSSASFYKWRAKYGGMDASLISQMKAMEEENRRLKRMYADLSMQADLLKEALGKKMTRPSQRREMAEK
AVARHGVSIALACRAFGVSETCYRYSPKLKDENEEIADLLVGLTDARKTWGFGLCFLHLRNVKGHPWNHKRVYRIYCALELNLRIKPRKRLKREKPEALA
VPDAPNLTWSMDFMADRLGDGRQFRLLNVLDDFNREGLGIEVDFSLPAERVIRSLDRIIEWRGKPGTIRVDNGPEYISGKLMEWAEKRKITIQHIQPGQP
QQNAYIERYNRTVRHEWLDQYIIETIEEAQDFATQWLWTYNNDRPNMGIGGITPAQKLKMAA
AVARHGVSIALACRAFGVSETCYRYSPKLKDENEEIADLLVGLTDARKTWGFGLCFLHLRNVKGHPWNHKRVYRIYCALELNLRIKPRKRLKREKPEALA
VPDAPNLTWSMDFMADRLGDGRQFRLLNVLDDFNREGLGIEVDFSLPAERVIRSLDRIIEWRGKPGTIRVDNGPEYISGKLMEWAEKRKITIQHIQPGQP
QQNAYIERYNRTVRHEWLDQYIIETIEEAQDFATQWLWTYNNDRPNMGIGGITPAQKLKMAA
Blast result :
Comments
References
1] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277