ISPak1

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
GQ871938 Y Paracoccus alkenifer
Paracoccus alkenifer DSM 11593
DNA section
IS Length : 853 bp

Ends


IR Length : 13/15

IRL : GGGTCAGGACCCATTGATTTCAGCCGCGCGGCATGATTCAGGCTCCGCAA
IRR : GGGTCAGGACTCGTTCTCGATTCATAGCCAGAACATGACGGTTGCGGCGA

Insertion site


Left flankDirect repeatRight flankDR Length
CCGGAGATCCTAAAAATGCGGA2

DNA sequence

GGGTCAGGACCCATTGATTTCAGCCGCGCGGCATGATTCAGGCTCCGCAAGGAGGCTGGATCATGAGCAACCTTTTCTGGCTGACGGATGCGCAGATGGC
CCGCCTTCAGCCGTTTTTCCCGAAGAGCCACGGCAAGCCGCGCGTCGATGACCGGCGTGTTCTCAGCGGCATTATCTTCATCAATCGCAATGGCTTGCGG
TGGTGCGATGCGCCCAAGGAGTATGGTCCACCGAAGACCCTCTATAATCGTTGGAAGCGCTGGGGCGAGAAAGGCGTCTTCGCCCGGATGATGGAAGGCC
TGGCCTCCGAGGCGGCCGTTCCGAAGACGGTGATGATAGACGCGACCTATCTGAAGGCGCATCGCACGGCCTCCAGCCTGCGGGCGAAAAAAGGGGGCCC
GGCGACCAGAGGGGCCGCCTGATCGGCCGCACCAAAGGCGGCATGAACACCAAACTGCACGCCGTCACCGACGCTGATGGGCGGCCGATCCGGTTCTTCA
TGACTGCAGGTCAGGTGAGCGACTACAGCGGTGCTGCGGCCCTGTTGGGCAGCTTGCCGGCGGCAGAATGGCTGCTGGGCGACCGTGGCTATGATGCCGA
TTGGTTCAGAGATGCGTTGAAAGACAAGGGGATTAAGCCCTGCATACCGGGACGGAAGTCCCGTGGAAAACCCGTCAAGTATGACAAGCGCCGCTACAAA
CGCCGCAACCGGATCGAGATCATGTTCGGGCGGCTGAAGGACTGGCGACGGGTCGCAACCCGTTACGACAGATGCCCGAAGGTCTTCCTCTCAGCCATCG
CCCTCGCCGCAACCGTCATGTTCTGGCTATGAATCGAGAACGAGTCCTGACCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
390 bp129 aa33422+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MIQAPQGGWIMSNLFWLTDAQMARLQPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPPKTLYNRWKRWGEKGVFARMMEGLASEAAVPKTV
MIDATYLKAHRTASSLRAKKGGPATRGAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
582 bp193 aa251832+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LEALGRERRLRPDDGRPGLRGGRSEDGDDRRDLSEGASHGLQPAGEKRGPGDQRGRLIGRTKGGMNTKLHAVTDADGRPIRFFMTAGQVSDYSGAAALLG
SLPAAEWLLGDRGYDADWFRDALKDKGIKPCIPGRKSRGKPVKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAATVMFWL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
800 bp266 aa33832+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIQAPQGGWIMSNLFWLTDAQMARLQPFFPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPPKTLYNRWKRWGEKGVFARMMEGLASEAAVPKTV
MIDATYLKAHRTASSLRAKKRGPGDQRGRLIGRTKGGMNTKLHAVTDADGRPIRFFMTAGQVSDYSGAAALLGSLPAAEWLLGDRGYDADWFRDALKDKG
IKPCIPGRKSRGKPVKYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPKVFLSAIALAATVMFWL

 

Blast result :
Comments
References
1] Bartosik, D. (2010) Direct submission.

2] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277