ISVsp13
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ABIZ00000000 | ND | Verrucomicrobium spinosum | Verrucomicrobium spinosum DSM 4136 |
DNA section
IS Length : 1503 bp
Ends
IR Length : 25/32
IRL : TGAGGTGGACCCCGGAAATCGGACCAGTGCTGAAGTTGGAGTTTTGGAGT
IRR : TGAGGTGGACCCCGTTCTTCGGCCAGAGTTGCTGGAAGTTAAGCTATGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAGACTAAT | CTT | CTATGGAGCG | 3 |
| GGATATTGGC | GGC | AATGATGTGT | 3 |
| TTGGACAGAA | TAT | TGCCATGTTT | 3 |
DNA sequence
TGAGGTGGACCCCGGAAATCGGACCAGTGCTGAAGTTGGAGTTTTGGAGTTGGGCAGGGCTGGAGTAAACCAGAACCTGAAACTCGAAAATGAAAGCCAA
ACGTAGAAGACACGACCCCGAATTCAAGGCCCGAGTGGCGCTCGAAGCGCTCAAAGGCCTCAAGACCATTGCCGAGATTGCCAAGGAGTACGACATCCAT
CCTGTGCAGGTCTCTGACTGGAAGAAGACGCTCCAGGCTGGGATGGCCGGGGTCTTTGGTCAGGACCATGGCCGTGCCGACCAGGAGGAGCACGAGCGCG
AACGGGCCCAGTTGCACTCCAAGATCGGGGAGCTGGCCGTGAAAGTGGACTTTCTGCAAAAAAAGTCCAAACAGCTCGGCATCTGGAACGACCTGCCGGG
CTGGTCGAAAAAGAACACCCCGTTTTGAGCATGAGAAGCCAGTGTGAACTGCTGGGGGTGTGCCGCTCGATGCTAGCCTATGAGGCGGTGCCTGAGAGCG
GTGAAGACCGCCGAATCATGAGGTTGCTTGATGAGCTCTACCTCAAGGATCCGTGCCTGGGGACCCGTCGCCTGGTGAAGGTGCTGGAGCGCGACCACGG
CTTCAAGGCCAACCGCAAGCGCTTGCAGCGGCTGCGACGTCAGATGGGGCTGGAGGCCATCTACTGTCGTCCGCGCACGAGCCAGCCTGGAGACGGGCAC
CGCATTTATCCGTACTTGCTGCGGGAGATGGCCGTGGAGCGTCCCGACCAGGTGTGGTGCGCCGACATCACCTATGTGCCCATGGCGCGAGGCCACGCCT
ATCTGTGTGCAGTGATGGACTGGCACACGCGCAAAGTGCTGGGGTGGCAGGTGAGCAACACGATGGATGTGAACCTGTGTCTTGAGGCGCTGGAGATGGC
GGTGAGGGAGACAGGGAGCAGGCCGGAGATCTTCAACACCGACCAGGGCAGCCAGTTTACCTGTGAGCAATGGACCGGGCGGATTGAAGAGCTGGGAGCG
CGCATCAGCATGGACGGCAAAGGGCGGTGGATGGACAACGTGTTCATCGAACGGCTGTGGCGGAGCGTGAAGTACGAGGAAATCTACATACGCGAACATG
GCACCATCCTTGAGCTTGAGCGGGGGCTGGCCCGGTGGTTTGAGCGCTACAACACATGGCGTCCCCACGAGGCCCTGGGCTACCAGACACCTGAGCAAGC
CTACAAGGGCATTGCGAAGCTGCCTGCGACAGTGCCGAGGATGCCCATGGCTCTTGCCGCATGAACACACCAAGCGGCCTTCCAGAGCCCGCTTGGATGC
CCCCCAGGGGCGGCGGTCCGACCGAAGGGGTTGTTGCACTTCAGGCTCTCCGCTTCGCTCCGAGCCTTTCGTTACACAACCCCATCGGTCGGAAGTGCAA
TGAGGAAGTTGACATCAAGACAGAACCAAACTAACCCAATGAACAGCCAGCCCACCATAGCTTAACTTCCAGCAACTCTGGCCGAAGAACGGGGTCCACC
TCA
ACGTAGAAGACACGACCCCGAATTCAAGGCCCGAGTGGCGCTCGAAGCGCTCAAAGGCCTCAAGACCATTGCCGAGATTGCCAAGGAGTACGACATCCAT
CCTGTGCAGGTCTCTGACTGGAAGAAGACGCTCCAGGCTGGGATGGCCGGGGTCTTTGGTCAGGACCATGGCCGTGCCGACCAGGAGGAGCACGAGCGCG
AACGGGCCCAGTTGCACTCCAAGATCGGGGAGCTGGCCGTGAAAGTGGACTTTCTGCAAAAAAAGTCCAAACAGCTCGGCATCTGGAACGACCTGCCGGG
CTGGTCGAAAAAGAACACCCCGTTTTGAGCATGAGAAGCCAGTGTGAACTGCTGGGGGTGTGCCGCTCGATGCTAGCCTATGAGGCGGTGCCTGAGAGCG
GTGAAGACCGCCGAATCATGAGGTTGCTTGATGAGCTCTACCTCAAGGATCCGTGCCTGGGGACCCGTCGCCTGGTGAAGGTGCTGGAGCGCGACCACGG
CTTCAAGGCCAACCGCAAGCGCTTGCAGCGGCTGCGACGTCAGATGGGGCTGGAGGCCATCTACTGTCGTCCGCGCACGAGCCAGCCTGGAGACGGGCAC
CGCATTTATCCGTACTTGCTGCGGGAGATGGCCGTGGAGCGTCCCGACCAGGTGTGGTGCGCCGACATCACCTATGTGCCCATGGCGCGAGGCCACGCCT
ATCTGTGTGCAGTGATGGACTGGCACACGCGCAAAGTGCTGGGGTGGCAGGTGAGCAACACGATGGATGTGAACCTGTGTCTTGAGGCGCTGGAGATGGC
GGTGAGGGAGACAGGGAGCAGGCCGGAGATCTTCAACACCGACCAGGGCAGCCAGTTTACCTGTGAGCAATGGACCGGGCGGATTGAAGAGCTGGGAGCG
CGCATCAGCATGGACGGCAAAGGGCGGTGGATGGACAACGTGTTCATCGAACGGCTGTGGCGGAGCGTGAAGTACGAGGAAATCTACATACGCGAACATG
GCACCATCCTTGAGCTTGAGCGGGGGCTGGCCCGGTGGTTTGAGCGCTACAACACATGGCGTCCCCACGAGGCCCTGGGCTACCAGACACCTGAGCAAGC
CTACAAGGGCATTGCGAAGCTGCCTGCGACAGTGCCGAGGATGCCCATGGCTCTTGCCGCATGAACACACCAAGCGGCCTTCCAGAGCCCGCTTGGATGC
CCCCCAGGGGCGGCGGTCCGACCGAAGGGGTTGTTGCACTTCAGGCTCTCCGCTTCGCTCCGAGCCTTTCGTTACACAACCCCATCGGTCGGAAGTGCAA
TGAGGAAGTTGACATCAAGACAGAACCAAACTAACCCAATGAACAGCCAGCCCACCATAGCTTAACTTCCAGCAACTCTGGCCGAAGAACGGGGTCCACC
TCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 339 bp | 112 aa | 90 | 428 | + | No |
Description : First part of the transposase
ORF sequence :
MKAKRRRHDPEFKARVALEALKGLKTIAEIAKEYDIHPVQVSDWKKTLQAGMAGVFGQDHGRADQEEHERERAQLHSKIGELAVKVDFLQKKSKQLGIWN
DLPGWSKKNTPF
DLPGWSKKNTPF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 302 | 1264 | + | No |
Description : Second part of the transposase
ORF sequence :
TGPVALQDRGAGRESGLSAKKVQTARHLERPAGLVEKEHPVLSMRSQCELLGVCRSMLAYEAVPESGEDRRIMRLLDELYLKDPCLGTRRLVKVLERDHG
FKANRKRLQRLRRQMGLEAIYCRPRTSQPGDGHRIYPYLLREMAVERPDQVWCADITYVPMARGHAYLCAVMDWHTRKVLGWQVSNTMDVNLCLEALEMA
VRETGSRPEIFNTDQGSQFTCEQWTGRIEELGARISMDGKGRWMDNVFIERLWRSVKYEEIYIREHGTILELERGLARWFERYNTWRPHEALGYQTPEQA
YKGIAKLPATVPRMPMALAA
FKANRKRLQRLRRQMGLEAIYCRPRTSQPGDGHRIYPYLLREMAVERPDQVWCADITYVPMARGHAYLCAVMDWHTRKVLGWQVSNTMDVNLCLEALEMA
VRETGSRPEIFNTDQGSQFTCEQWTGRIEELGARISMDGKGRWMDNVFIERLWRSVKYEEIYIREHGTILELERGLARWFERYNTWRPHEALGYQTPEQA
YKGIAKLPATVPRMPMALAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1175 bp | 391 aa | 90 | 1264 | + | Yes |
Chemistry : DDE
ORF sequence :
MKAKRRRHDPEFKARVALEALKGLKTIAEIAKEYDIHPVQVSDWKKTLQAGMAGVFGQDHGRADQEEHERERAQLHSKIGELAVKVDFLQKKVQTARHLE
RPAGLVEKEHPVLSMRSQCELLGVCRSMLAYEAVPESGEDRRIMRLLDELYLKDPCLGTRRLVKVLERDHGFKANRKRLQRLRRQMGLEAIYCRPRTSQP
GDGHRIYPYLLREMAVERPDQVWCADITYVPMARGHAYLCAVMDWHTRKVLGWQVSNTMDVNLCLEALEMAVRETGSRPEIFNTDQGSQFTCEQWTGRIE
ELGARISMDGKGRWMDNVFIERLWRSVKYEEIYIREHGTILELERGLARWFERYNTWRPHEALGYQTPEQAYKGIAKLPATVPRMPMALAA
RPAGLVEKEHPVLSMRSQCELLGVCRSMLAYEAVPESGEDRRIMRLLDELYLKDPCLGTRRLVKVLERDHGFKANRKRLQRLRRQMGLEAIYCRPRTSQP
GDGHRIYPYLLREMAVERPDQVWCADITYVPMARGHAYLCAVMDWHTRKVLGWQVSNTMDVNLCLEALEMAVRETGSRPEIFNTDQGSQFTCEQWTGRIE
ELGARISMDGKGRWMDNVFIERLWRSVKYEEIYIREHGTILELERGLARWFERYNTWRPHEALGYQTPEQAYKGIAKLPATVPRMPMALAA
Blast result :
Comments
Genome in progress.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISVsp13 is 68% aa similar to ISAli5.
There are 4 copies of ISVsp13 in the chromosome of Verrucomicrobium spinosum DSM 4136.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISVsp13 is 68% aa similar to ISAli5.
There are 4 copies of ISVsp13 in the chromosome of Verrucomicrobium spinosum DSM 4136.
References
1] DeBoy, R. (2010) Direct submission