ISVsp4

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_ABIZ00000000 ND Verrucomicrobium spinosum
Verrucomicrobium spinosum DSM 4136
DNA section
IS Length : 2518 bp

Ends


IR Length : 18/22

IRL : GTAAGCAGATCAGCAAGGCCGTCTGGTGCGCGGAGCGCAGCCGGGCAAGT
IRR : GTATGCAGTTCAGCAATCCCGTGGCATAGCTTCAGGTTCGAGCCCTGACG

Insertion site


Left flankDirect repeatRight flankDR Length
GAACCCTGCTGTTCAACTACAGGGTCCG8
CACGAAGGCGCTCCTTGTCCTTCATGAA8
GCTCGATGTTCGTTGACACGTTGCAAGT8
GGCGGCAGTCTTCTTTGGTGCGGTGCTG8

DNA sequence

GTAAGCAGATCAGCAAGGCCGTCTGGTGCGCGGAGCGCAGCCGGGCAAGTTTGCGCGTATGCCTCGATATACAGCCCTGCAACGTCGTCGTCTAGTGCGC
CAGTTTCAATCCAGCGGCCTGACCCTGGCAACTTTTTGCCGGCTTCATTCTTTGAGTGTCTCAAGCCTTTGTGCCTGGCGTCGCCATGTTGAACTTCAGA
AGCGCTCCTCTGATCCTGCGCCGCCTCCATGGCTGCCGGTGGAAGTTTCCTCACCTCCGGGGAGCACTGCTGCGGGACTTCCCGGCTTGGATTATCTCAT
CGAAGCGGGTCTCTGGCGTCTGCACGTCCCTTCAGGCTTTGGCCGCGATGAAGTGGCCGCCCTCCTTGAACTTCTCCATGCCAGGCAGGGAGGTGCCCCA
TGCTAAGCCTGGGTCCTGCCACCAAAGTCCATTTGCTGGCAGGCGCCACCGACATGCGCCTTGGGTTTGAGGGGCTGCTCGCCCTGGCTTCTGGTCTGTT
GCGCGAGGATCCTTTGAGCGGTCACCTCTTTGTCTTTTGCAACAAGCACCGCACCCGTCTCAAGGCCCTCTACTGGGACGGCTCGGGCTTGTGGGTGTGC
GCCAAGCGGCTGGAGAAGGGGCGCTTCCACTGGCCGCAGCCCCCAGCGCAAGGAGCTTCACGCAAGGTGGTGCTCACCCAGGCGGAGCTCACTTTGCTGC
TGGCGGGCATTGACCTGGAAGGCACCCAACGGAGGGCCTGGCACCGGGTCGGGTGAGGGTGCGGGAAGAGGTGTTTTTTAGGGTTCGCGGTAACAACTTG
TTGCAACGAGCCCGCTGCTGGGCATCATGCAGCTCCCGCTGCTCATGCCCGCCACTCCTGCCACCCCGGATCTGCCTGAAGCCCTGTTGCTTTCTTTGGG
ACCCCGGGAGCGTCTTGCCCTGGAGGAGATCCTGCGTCAGCGCGATGAGCGTATTGCCACCCTGGAACTGGAAGTGCGCCTGCGTGAGGAGCAGTTGCGG
CTGGCCCTCATCAAGAAGTACGGTCCCAAAGGCGAGGGCCTCGGCAAAGACCAGGCCCTGCTGCTGGATCTGGAGCCCGGCGTGCAGGAGGCCGAGGTGG
CCCTTGAAGTGGGCCTGGCCCTGGCGGACAAGACCCTGCCCGAGGCTGGACGCCTGGAACAGGAGCAGGCCGCCCGGCTCAAAAAGAAAAAGCCCGGCAC
CCCGCGCTACGCCCAAGTGCATCCTGGGCGGCACGAACTGCCCGCGCATCTGCCGCGGGTGGAAGTCATCCTACCATGCTTGGAAGCGGCGCAAGGCGAA
CTGGTGGGCTATGAGATCAAGGAAGAGCTCGTCATCAAGCCTGCGGAGTTCTTTGTGCGGGTGCTCAAGCGCGAGAAGCGAGTCATCCAGCTGGGGGACC
GTCGCACGGTGGCCACAGCCGCCTCCCCTGGGCGCATCGTCGACAAAGGGCAGCTGGCCAACGAGACGGTGGTGGAGCTGGTGGTGCGCAAGTACGCCGA
TTATCTGCCGGTGTACCGTCAGCTACAGGGGTGGGAGCGCGATCACAGTGTGACCGTGCGTCAGGCCACGGCGACGCGGGCGGTGATGGCCGCAGGAGCG
TTGCTGCAACCTCTGGCGCGGGCCATCGGCCAGGAGCTGCGGCAGGGGCCGCTCATTCAGGCCGATGAGACGAGGCTGCCGGTGCTGCAAGATCTGGGTA
AAGGCCGCAACGATGTGGCGTGGTTGTGGCAGTACTCCATCCCCGGAGGGCTGGTGTACTTCGAGTACCAGGACAACCGTGCCCAGGCGGGGGCGAGGGC
CTATTTGAAGGACTACGGAGGCATCCTGCAAAGCGACGGCTATGTGGTCTATGACTGTCTGGAGGGGCAGGTGCAGCGGCATGCGGGATGTTTTGCCCAC
GTGCGACGCAAGTTCGTGGAGGCGTGCCAGGCCGCCCCCAAGGAAGTGCCGTGCGAACCGGGGCTGGCGGTGGTGGCCAGCATCGGCGCGTTGTATGGAG
TTGAAGAGCGGGCGCGGGAAAAGCAGTTGAAGGGGCAGGCCCGTCTGGACTACCGGCAGGAGCAGGGTGTGGCGCAAAAGCTCTCCAGCCTCAAGGCGCA
GATCCTGGAGGTGCGGGCCAAGGCCCTCCTGCCGCAGAGCCTGCTGGCCAAGGCCTGTGACTATGCGCTCAACCAGTGGGAGAAGCTGGAGGTGTATGCC
AGCCACGGGGAGGTGGAGATAGACAACAACTGGTGTGAGAATGCCATGAGGCCGGTGGCGCTGGGGCGCAAGAACTGGCTGCATCTAGGAAGCCACGAGA
GCGGGCCCAAAGTAGCGGCGATCCTGACGGTGCTGGCCAGTGCTCAGCGGCTGGGGCTCAACGTGCGGGAGTATCTTGGGGAGGCGCTGGAGACCCTGTG
TGACGGTGAGGGGTTCAACATCACGCGCATCGGGGAACTGCTGCCCAGCCGCTGGAAGCCCAAACCTGCGTCAGGGCTCGAACCTGAAGCTATGCCACGG
GATTGCTGAACTGCATAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
348 bp115 aa59406+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MPRYTALQRRRLVRQFQSSGLTLATFCRLHSLSVSSLCAWRRHVELQKRSSDPAPPPWLPVEVSSPPGSTAAGLPGLDYLIEAGLWRLHVPSGFGRDEVA
ALLELLHARQGGAPC

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
357 bp118 aa400756+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MLSLGPATKVHLLAGATDMRLGFEGLLALASGLLREDPLSGHLFVFCNKHRTRLKALYWDGSGLWVCAKRLEKGRFHWPQPPAQGASRKVVLTQAELTLL
LAGIDLEGTQRRAWHRVG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1536 bp511 aa9742509+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRLREEQLRLALIKKYGPKGEGLGKDQALLLDLEPGVQEAEVALEVGLALADKTLPEAGRLEQEQAARLKKKKPGTPRYAQVHPGRHELPAHLPRVEVIL
PCLEAAQGELVGYEIKEELVIKPAEFFVRVLKREKRVIQLGDRRTVATAASPGRIVDKGQLANETVVELVVRKYADYLPVYRQLQGWERDHSVTVRQATA
TRAVMAAGALLQPLARAIGQELRQGPLIQADETRLPVLQDLGKGRNDVAWLWQYSIPGGLVYFEYQDNRAQAGARAYLKDYGGILQSDGYVVYDCLEGQV
QRHAGCFAHVRRKFVEACQAAPKEVPCEPGLAVVASIGALYGVEERAREKQLKGQARLDYRQEQGVAQKLSSLKAQILEVRAKALLPQSLLAKACDYALN
QWEKLEVYASHGEVEIDNNWCENAMRPVALGRKNWLHLGSHESGPKVAAILTVLASAQRLGLNVREYLGEALETLCDGEGFNITRIGELLPSRWKPKPAS
GLEPEAMPRDC

 

Blast result :
Comments
ISVsp3 orfA is 58% aa similar to ISSwo2, orfB is 65% aa similar toISSba7 and ISVsp3 orfC (the transposase) is 53% aa similar to ISThsp3.
There are 4 copies of ISVsp4 in the chromsome of Verrucomicrobium spinosum DSM 4136. One copy carries an insertion of a gene for reverse transcriptase within transposase orf3.
References
1] DeBoy, R. (2010) Direct submission