ISfinder Mirror Site

ISRssp4

Family IS30
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Roseovarius sp.	Roseovarius sp. 217

DNA section

IS Length : 1110 bp

Ends

IR Length : 24/28

IRL : GTGAATTCACAAATGAAGTGCAATTTCTTTAACAAGGTATTGAATTGCAT
IRR : GTGAACTCACGCTGAAGCGCAATTTCTGTGGGGTCATTTTATCTGGATCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATGTACGCCG		AGCAAAGTTA	0

DNA sequence

GTGAATTCACAAATGAAGTGCAATTTCTTTAACAAGGTATTGAATTGCATGGAGTTAATTTATGGGACGCTGCTACCCTCACCTGAACCTCGACGAACGG
CGTAATCTGGCCAAATGGCTTGAAGCGAAGATATCAATCAAAGAGATCGCTGATCGGTTGTGCAGAGATGCATCGACAATCTACCGCGAGATCAAGCGCA
ACTCGTGCCGTTTCGACGATCAACCTGAGTTGAACGGCTATCATGCGTTGGCCGCGCAGGATCGGAGCGAAGATCGCCGTGCGGTGCATCGCAAGCTGAT
CTTATATCCCGAGATCATGGCTGCCGTGCGGTGTGGTTTTGACGCCGGATGGTCCCCTGAACAAATTGCAGGACGTATGCGCCTGGAACGCCATCCGATG
CGTGTGAGCCACGAGACGATCTATCGATATGCCTATTCAAAAGCCGGCCGTGCTGAGAAGTTCTACCGCCACTTACCCATGCATCGCCATCGCCGCAGGC
CACGTGGAACTCGGAGGCATCATGGGCGTCATATCCTTGATGAATTGGCCATCGCGCACCGCCCTGAAGCGATAGCCGAACGTGACCAGTTCGGTCACTG
GGAGTGCGACCTGGTGATGTTCCGCAAGGAGTTTGGCAAAGCCAATGTGACGTCTCTGGTCGAGCGTGTCAGCCGGTTTGCTCTCGTTTTGAAGAACCCG
GATCGCCAGTCAAAACCGGTGATGGAGAGCCTGATCGCCAACCTGTCTCCCCTGCCAGCTGAAGCGCGTCGCAGCATCACCTTTGATCGCGGCACTGAGT
TCAGCGCATGGCAGCATCTAAAGGATGGTCTGGGCGTCGAACCATGGTTCTGCGATCCTCAGTCGCCGTGGCAAAAAGGAACGGTCGAGAACACCAACAA
TCGCCTGCGCCGCTACCTGTCCCGCAAGGCTGATCCCACGGCGTTCACAGATCGATATTTGAGGTCAATCTGTGACCGCCTCAACGCGACCCCACGCAAG
TGTCTGGGGTATCAAACACCGGCTGAGGTATTCCGGGCAAAACTGATGGAAGGAGGGCCATGATCCAGATAAAATGACCCCACAGAAATTGCGCTTCAGC
GTGAGTTCAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1002 bp	333 aa	62	1063	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGRCYPHLNLDERRNLAKWLEAKISIKEIADRLCRDASTIYREIKRNSCRFDDQPELNGYHALAAQDRSEDRRAVHRKLILYPEIMAAVRCGFDAGWSPE
QIAGRMRLERHPMRVSHETIYRYAYSKAGRAEKFYRHLPMHRHRRRPRGTRRHHGRHILDELAIAHRPEAIAERDQFGHWECDLVMFRKEFGKANVTSLV
ERVSRFALVLKNPDRQSKPVMESLIANLSPLPAEARRSITFDRGTEFSAWQHLKDGLGVEPWFCDPQSPWQKGTVENTNNRLRRYLSRKADPTAFTDRYL
RSICDRLNATPRKCLGYQTPAEVFRAKLMEGGP

Blast result :

Comments

ISRssp4 is 63% aa similar to ISRle2.
Genome in progress : Accession number : NZ_AAMV01000007.

References

1] ISfinder annotation (2010)
2] Murrell,J.C., Schafer,H., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.