ISfinder Mirror Site

ISHwa5

Family IS4
Group ISH8

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
FR746099	ND	Haloquadratum walsbyi	Haloquadratum walsbyi

DNA section

IS Length : 2256 bp

Ends

IR Length : 18

IRL : CACCCGTTATCGCTTTAGCAGGATAGCCATCAGCATGATCAATGCTATCA
IRR : CACCCGTTATCGCTTTAGGTCCAGATGACCGTTCAGGTCGGTAGCTTGGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCGTCGTCAT	CATCTAGTTAT	ACACTGTGCG	11

DNA sequence

CACCCGTTATCGCTTTAGCAGGATAGCCATCAGCATGATCAATGCTATCAGGACCGCGACTGTCTGTGAAGCTCCTTTCTACAATCCCAGTTCTCGTCGA
AGCTCCTTTTTCGCGTCAATATCGCCGGATGCGTGACCGAGAGCAAGGTCTGTGACTACTGACTGCCAGTCTTTGACTTGGTCCATGAGTTCTTCGTAGA
CTTGGTCTTCTGTTTTGTCCCCAACATCCACCGTTCGAACTGCGATCCAGCCTCGTTGAGGGAAATATTCGATCACGGCGCCACCCGTCTCCGTCTGTAA
CGCGATGCGTTCGGTCAGTCCCGTTCCGGGACTGCCGCTCATCAACGGCAACGCAGTTCGTATCTTCTCGGCGTTTGCCACTGCTTCAACCGTCTCAACC
GGGAGTGACTCATCAGGAAACGTTGAGAGCACGTCTCCCTGTGTAATTTCTCCCATCTCTTCGACGATTTTCTTGAGGTGATCTGGTGCGTCAAGAGCAG
GACCAGGACAATCGTGGAGCCGAAGCTTCGTCAACGTCTGGAACGTCTCACCGCACGCGTCGCATGTGTGGGCCATTGGTCACTCTCAACTGTGAGTTCG
CACCCATCCCAAATTGGTCTTTTCCTACCTCGTCTCTCCAAACGGTAGACTTCTTTCCGTCTTTCCGGTCTATTGAGACATGAAGCGGTGGCTTTACCGA
ATTGTCCAGTCACTTCGGACAGCACTTCCAGAAGAGGCCGTGGCGCGAACTGCTCGCGCCACGGACCTCGTTCAACGAGGTCGAGAAATCGGATCAGTGC
CGTTTCTCTGGTCGTTCCTCTTTGGAACGACACAGTCAGACGGCTCTGTAACCAAGGTACGGAACTTCTACGAGGCGTTCACTGATCACGACGCCGCCTA
TTCGTCGATCCAGCAGTGGATCACGCCCGAACTCAAGCAACTGCTCAGCGAGACGTTTGCTCACCTCAACATCGAACTCGGCGCAACAGAATCTTCTCTC
GGCGGGCGGTTTGATCGCTTCCGTGATGTCTTCATCACAGATGCGACTGACTGCACGCTCTCAGCAGCTTCCTTCGAGGATTTTCCCGGCTACAGCGACG
ATCATGCTGGAGCACAGCTCCACATGATCGAATCACTCGCTTCGCGTGCACCGTTTTTCGCCTCAATCACCGACGTTCGTACTGACGAGTTGAGCCAACT
CCAGATCGGTGACTGGGTTGCTGATTCACTGCTGTTGGACGATCTTGGCTACCACGACTACGGCAAGATTGCACGGATTGACGAGCTTGGTGGCTGGTTC
GTGAATCGGCTGAAAGTCAACTCAAACGGCCCGATTACCGAAGAACTCGAGAGATGGCCCACAGGTGCCATCTCTCTGGAAGGCACCCACATCCAAGATG
TCCTTCCAGACCTCTACCGCAGCGAAATCGACGCACTCGCGCAGTTCGATACACCAAGTTCAGACAGCGAACTTCTCCCCTGTGAGTTCCGCCTCGTCGT
GACGGAGTCCGACGAGGCGGCTGATGAAGAAGACGCACCTGACGCCGATCACGAGTATCACCTGTACGTGACGAATCTCCCGAAAGAGTGGTTCTCGCCC
CGGGAAGTCGCAGCGTTGTACTCTGCGAGATGGAGCATTGAAACGCTCATTCAGGAAGCGAAATCCGTGTTCGGCCTTGACGAAATCTCGGTCCGGACGA
CGGAATCGATCGAGTGTTTCATGCTGGCGTCGATGGTGATGGTACTGCTCAGCCGGTACTTGTTGCGGCAGATTCGAGCAGGGCTCGGATCTGCCCGCGA
TAGCTCCGTTGAGGAAGAGACTGAGACACAGCCGATGTCGTTCTCGAAGCGTCTTCGGGCGTTTGGAACGGATATTCTGAGGATGCTGGCCGAGCAGTTT
GGATACTCGGGGGAACCGGGGGAAACGATCGTCAAGAGTGCTATTGATCGAAATGTGAACCGCCACGCGCTCACGGAGCGAGTGGCGTACGGGACCGTTG
ATCCAAACCTCACGAAGGCCGGAGAATTGGCGACAATCCGGCCCGGCTGAACGCGTCAGCCGGGCCGGGTAATGCCGCAGTGAGTGGCTACACTCTTGGT
CACACAGCTCTCCGACGGTGTTTCACTCCTCCACCGAGACCCGGAGTGTTTTGCTGATTTTGACCTGCGTAAGTCGCGTTCCACACTTCAGCAGCACCGT
AGACGCTCCAAGCTACCGACCTGAACGGTCATCTGGACCTAAAGCGATAACGGGTG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
75 bp	24 aa	576	502	+	No

ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1371 bp	456 aa	680	2050	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKRWLYRIVQSLRTALPEEAVARTARATDLVQRGREIGSVPFLWSFLFGTTQSDGSVTKVRNFYEAFTDHDAAYSSIQQWITPELKQLLSETFAHLNIEL
GATESSLGGRFDRFRDVFITDATDCTLSAASFEDFPGYSDDHAGAQLHMIESLASRAPFFASITDVRTDELSQLQIGDWVADSLLLDDLGYHDYGKIARI
DELGGWFVNRLKVNSNGPITEELERWPTGAISLEGTHIQDVLPDLYRSEIDALAQFDTPSSDSELLPCEFRLVVTESDEAADEEDAPDADHEYHLYVTNL
PKEWFSPREVAALYSARWSIETLIQEAKSVFGLDEISVRTTESIECFMLASMVMVLLSRYLLRQIRAGLGSARDSSVEEETETQPMSFSKRLRAFGTDIL
RMLAEQFGYSGEPGETIVKSAIDRNVNRHALTERVAYGTVDPNLTKAGELATIRPG

Blast result :

Comments

ISHwa5 orfB (the transposase) is 51% aa similar to ISH5.
ORF1 is a passenger gene and seems to be a gene remnant it shows 48% seq_id to conserved hypothetical proteins, which commonly are 330 aa long and occur in several halophiles.

References

1] Dyall-Smith,M.L., Pfeiffer,F., Klee,K., Palm,P., Gross,K., Schuster,S.C., Rampp, M., and Oesterhelt,D. (2011) PLoS One 6: e20968