ISfinder Mirror Site

ISTel4

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s) ISEL2

Accession number	Transposition	Origin	Host
NC_004113	ND	Thermosynechococcus elongatus	Thermosynechococcus elongatus BP-1

DNA section

IS Length : 842 bp

Ends

IR Length : 17/20

IRL : GATAGTGCCGTCACGAGTTTATGAGATACTGAGCGTGTGTCTGGACTGTA
IRR : GATACTGTCGTCACGAGATTGATGCCCAAATGGCGATACAACGGATGTGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CACGAAACAT	TA	GAGGCCCATC	2
TCCCGTTTCT	TA	GGCTCCACCT	2
GACTTTGAGT	TA	GTTCTCCCCG	2
CCCGATAGCC	TA	GAGAACCTAG	2
TTGTTGATCC	TA	AGGGATGGCG	2
ATGCTCACGT	TA	GCATTGGGGT	2
AATCTTGGCC	TA	GAGGAGCAGT	2
GCCCTAGCCC	TA	GAACCAGTCG	2

DNA sequence

GATAGTGCCGTCACGAGTTTATGAGATACTGAGCGTGTGTCTGGACTGTATTATAGGACCGTGCCATGAGGACACCTGACTACCGTCGCCACGATATTTC
CGACCGGGTTTGGGAGCGGTTGGAACCGCATCTACCTGGACGCCGAGGGAGCTGGGGGGGTGTGGCGAAAGACAACCGGCAGTTCATCAATGCGGTGTTC
TGGATACTGCGCACCGGCGCCCCTTGGCGGGATTTACCCCCGGAGTACGGAGATTGGAAGAACGTGCATCGAAGGTTCTGCCGCTGGCGAGACAAAGGCG
TGTGGGAAAAGGTCCTTGAGCAGTTGATCGACGAGCCCGATTACGAATGGTTGATCATCGATGCTACTCATGTCAAAGTCCACCCTCATGCCACAGGGGC
CAAAGGCGGCAACCAAGACATGGGCCGTACAAAAGGGGGCTCAATACCAAGATACATCTGGCCGTGGATGCGCATGGTATGCCGCTGCGAATGGTTATCA
CAGCAGGTACCACTGCGGATTGTCGCCAAGCTGCAACCTTGATCGAAGGAATCGATGCAAAGTACCTGTTGGCGGACAAAGGCTACGACAGTGATGCCCT
CATCGCCCAAGCCAAAGAGCATGGGATGCAGCCTGTCATCCCTTCGCGCAGACATCGCAGACAGCGACGTGAATGCGACAAGCAGTTGTATCGGCTTCGT
CATCTGGTTGAGAATGCTTTTTTGCATCTCAAGCGTTGGCGAGGTATTGCCACGCGATACGCCAAAAATACGGCTTCTTTCCTTGCTGCTGTGCACATCC
GTTGTATCGCCATTTGGGCATCAATCTCGTGACGACAGTATC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
477 bp	158 aa	66	542	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRTPDYRRHDISDRVWERLEPHLPGRRGSWGGVAKDNRQFINAVFWILRTGAPWRDLPPEYGDWKNVHRRFCRWRDKGVWEKVLEQLIDEPDYEWLIIDA
THVKVHPHATGAKGGNQDMGRTKGGSIPRYIWPWMRMVCRCEWLSQQVPLRIVAKLQP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
513 bp	170 aa	320	832	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AVDRRARLRMVDHRCYSCQSPPSCHRGQRRQPRHGPYKRGLNTKIHLAVDAHGMPLRMVITAGTTADCRQAATLIEGIDAKYLLADKGYDSDALIAQAKE
HGMQPVIPSRRHRRQRRECDKQLYRLRHLVENAFLHLKRWRGIATRYAKNTASFLAAVHIRCIAIWASIS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
767 bp	255 aa	66	832	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRTPDYRRHDISDRVWERLEPHLPGRRGSWGGVAKDNRQFINAVFWILRTGAPWRDLPPEYGDWKNVHRRFCRWRDKGVWEKVLEQLIDEPDYEWLIIDA
THVKVHPHATGAKGGNQDMGRTKGGLNTKIHLAVDAHGMPLRMVITAGTTADCRQAATLIEGIDAKYLLADKGYDSDALIAQAKEHGMQPVIPSRRHRRQ
RRECDKQLYRLRHLVENAFLHLKRWRGIATRYAKNTASFLAAVHIRCIAIWASIS

Blast result :

Comments

ISTel4 is 86% aa similar to ISMasp6. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2010)
2] Nakamura,Y., Kaneko,T., Sato,S., Ikeuchi,M., Katoh,H., Sasamoto,S., Watanabe,A., Iriguchi,M., Kawashima,K., Kimura,T., Kishida,Y., Kiyokawa,C., Kohara,M., Matsumoto,M., Matsuno,A., Nakazaki,N., Shimpo,S., Sugimoto,M., Takeuchi,C., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (4), 123-130.